Antioxidant and antiarthritic potential of berberine: In vitro and in vivo studies

Jain, Shweta; Tripathi, Shalini; Tripathi, Pushpendra Kumar

doi:10.1016/j.chmed.2023.02.007

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…Berberine (BBR), as an isoquinoline alkaloid, possesses antimicrobial activity against Mtb and M. smegmatis. , Coptisine (COP), as a protoberberine alkaloid, shows antimicrobial activities againstEscherichia coli,Bacteroides fragilis, andClostridium perfringens at a low minimum inhibitory concentration (MIC) . Herein, we report the development of a new method effective against hard-to-target pathogens like mycobacteria based on biomimetic LrNs formulated from lipids extracted from mycobacterial membrane.…”

Section: Introductionmentioning

confidence: 99%

Lipids Extracted from Mycobacterial Membrane and Enveloped PLGA Nanoparticles for Encapsulating Antibacterial Drugs Elicit Synergistic Antimicrobial Response against Mycobacteria

Pu,

Wang,

Wang

et al. 2024

Mol. Pharmaceutics

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Tuberculosis (TB) is a chronic disease caused byMycobacterium tuberculosis (Mtb), which shows a long treatment cycle often leads to drug resistance, making treatment more difficult. Immunogens present in the pathogen’s cell membrane can stimulate endogenous immune responses. Therefore, an effective lipid-based vaccine or drug delivery vehicle formulated from the pathogen’s cell membrane can improve treatment outcomes. Herein, we extracted and characterized lipids fromMycobacterium smegmatis, and the extracts contained lipids belonging to numerous lipid classes and compounds typically found associated with mycobacteria. The extracted lipids were used to formulate biomimetic lipid reconstituted nanoparticles (LrNs) and LrNs-coated poly(lactic-co-glycolic acid) nanoparticles (PLGA-LrNs). Physiochemical characterization and results of morphology suggested that PLGA-LrNs exhibited enhanced stability compared with LrNs. And both of these two types of nanoparticles inhibited the growth of M. smegmatis. After loading different drugs, PLGA-LrNs containing berberine or coptisine strongly and synergistically prevented the growth of M. smegmatis. Altogether, the bacterial membrane lipids we extracted with antibacterial activity can be used as nanocarrier coating for synergistic antibacterial treatment of M. smegmatisan alternative model of Mtb, which is expected as a novel therapeutic system for TB treatment.

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Section: Introductionmentioning

confidence: 99%

Lipids Extracted from Mycobacterial Membrane and Enveloped PLGA Nanoparticles for Encapsulating Antibacterial Drugs Elicit Synergistic Antimicrobial Response against Mycobacteria

Pu,

Wang,

Wang

et al. 2024

Mol. Pharmaceutics

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“…Rheumatoid arthritis (RA), an autoimmune disease, causes persistent in ammation of the synovium inside joints. This in ammation affects the lining of the joints and may affect the ligaments and bones within the joints, leading to stiffness, discomfort, and degeneration of the articular cartilage (Jain et al 2023). The risk of developing RA between 30 and 50 years of age is 2 to 3 times higher for women than men (Shamlan et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Cartilage damage and joint alkalosishave been involved in the pathology of disease progression (Jain et al 2023).…”

Section: Introductionmentioning

confidence: 99%

“…The cause of joint damage has been found to be increased levels of pro-in ammatory cytokinessuch as tumor necrosis factor-alpha(TNF-α), interleukin-1-beta (IL-1β), interleukin-6 (IL-6), metabolic enzymes, and decreased levels of anti-in ammatory cytokines; interleukin-4(IL-4) and interleukin-10(IL-10) (Jain et al 2023).…”

Section: Introductionmentioning

confidence: 99%

“…Though several numbers of molecules, such as prostaglandins, TNF-α, IL-1β, and IL-6 are implicated in the pathophysiology of RA, reactive oxygen species (ROS) trigger the in ammatory processes by damaged articular cartilage, membrane lipids, proteins, and DNA (Jain et al 2023). It is observed that in ammatory cells such as neutrophils, lymphocytes, macrophages, and endothelial cells found in the in ammatory joint tissue are the main source of ROS (Sindhu et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Protective effect of Tecomastans (L.) Juss.exKunth in CFA-induced arthritic rat model

Das,

Kar,

Dash

et al. 2024

Preprint

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Tecoma stans (L.) Juss.exKunth (Bignoniaceae) is mainly found in tropical and subtropical regions of Africa and Asia. The leaves, flowers, roots, and bark are used to treat various aliments includes, skin infections, kidney problems, intestinal disorders, jaundice, toothaches, joint pain and repair cracked bones, antidotes for snake, scorpion, and rat bites. The aim of the study is to assess the anti-arthritic properties of T. stansleaf using Complete Freund's adjuvant (CFA)-induced rat model. The ethanol extract of T. stansleaf (ETSL) was taken for Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Mass Spectrometry (LC-MS) analysis for the identification of potential bioactive. The in vitro antioxidant and anti-arthritic activity was studied at concentrations of 25, 50, 100, 200, 400, and 500 μg/ml. In vivo anti-arthritic activity was carried out by administering CFA (0.1 ml) into the sub-plantar surface of the right hind paw. The experimental animals were treated with indomethacin (10 mg/kg) and ETSL (250, 500 mg/kg) once a daily for fourteen days. The arthritic parameters such as paw thickness, arthritic index, arthritic score, body weight, organ weight, and hematological and biochemical parameters were evaluated. Pro-inflammatory cytokines; tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β, anti-inflammatory cytokines; IL-4 and IL-10 and inflammatory mediator cyclooxygenase-2 (COX-2) were examined in blood serum. In vivo antioxidants parameters; superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and lipid peroxidation (LPO) was carried out in liver and joint. Radiological and histopathological analysis of joint was performed.A computational molecular docking investigation of the phytoconstituents was conducted against COX-2, IL-1β, IL-6, and TNF-α receptors by utilizing AutoDock 4.2 and BIOVIA-Discovery Studio Visualizer software. The in vitro result showed concentration dependent antioxidant activity with highest percentage of inhibition at 500 µg/ml. The in vivo result demonstrated significant restoration of arthritic parameters, hematological and biochemical indices and oxidative stress in CFA-induced rat which was further supported by radiological histological examination at ETSL 500 mg/kg. In addition, there was significant (p<0.05) reduction in pro-inflammatory cytokines, inflammatory mediators and up-regulation of anti-inflammatory cytokines was observed in the treated group. Verbascoside was found to exhibit better biding affinities -10.4, -7.4, -7 and -6.2 kcal/mol against COX-2, IL-1β, TNF-α, and IL-6 respectively, confirmed through in silico study. The observed outcome suggests that ETSL at a dosage of 500 mg/kg demonstrated notable anti-arthritic effects by suppressing pro-inflammatory cytokines and oxidative stress biomarkers. This effect could potentially be attributed to the presence of bioactive verbascoside identified in the LC-MS analysis.

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Unveiling gut microbiota's role: Bidirectional regulation of drug transport for improved safety

Wang,

Zhou

2024

Medicinal Research Reviews

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Drug safety is a paramount concern in the field of drug development, with researchers increasingly focusing on the bidirectional regulation of gut microbiota in this context. The gut microbiota plays a crucial role in maintaining drug safety. It can influence drug transport processes in the body through various mechanisms, thereby modulating their efficacy and toxicity. The main mechanisms include: (1) The gut microbiota directly interacts with drugs, altering their chemical structure to reduce toxicity and enhance efficacy, thereby impacting drug transport mechanisms, drugs can also change the structure and abundance of gut bacteria; (2) bidirectional regulation of intestinal barrier permeability by gut microbiota, promoting the absorption of nontoxic drugs and inhibiting the absorption of toxic components; (3) bidirectional regulation of the expression and activity of transport proteins by gut microbiota, selectively promoting the absorption of effective components or inhibiting the absorption of toxic components. This bidirectional regulatory role enables the gut microbiota to play a key role in maintaining drug balance in the body and reducing adverse reactions. Understanding these regulatory mechanisms sheds light on novel approaches to minimize toxic side effects, enhance drug efficacy, and ultimately improve drug safety. This review systematically examines the bidirectional regulation of gut microbiota in drug transportation from the aforementioned aspects, emphasizing their significance in ensuring drug safety. Furthermore, it offers a prospective outlook from the standpoint of enhancing therapeutic efficacy and reducing drug toxicity, underscoring the importance of further exploration in this research domain. It aims to provide more effective strategies for drug development and treatment.

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Antioxidant and antiarthritic potential of berberine: In vitro and in vivo studies

Cited by 9 publications

References 16 publications

Lipids Extracted from Mycobacterial Membrane and Enveloped PLGA Nanoparticles for Encapsulating Antibacterial Drugs Elicit Synergistic Antimicrobial Response against Mycobacteria

Lipids Extracted from Mycobacterial Membrane and Enveloped PLGA Nanoparticles for Encapsulating Antibacterial Drugs Elicit Synergistic Antimicrobial Response against Mycobacteria

Protective effect of Tecomastans (L.) Juss.exKunth in CFA-induced arthritic rat model

Unveiling gut microbiota's role: Bidirectional regulation of drug transport for improved safety

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