Antioxidant and Antibacterial Activities on Foodborne Pathogens of Artocarpus heterophyllus Lam. (Moraceae) Leaves Extracts

Loizzo, Monica Rosa; Tundis, Rosa; Chandrika, U.G.; Abeysekera, A.M.; Menichini, Francesco; Frega, Natale G.

doi:10.1111/j.1750-3841.2010.01614.x

Cited by 78 publications

(66 citation statements)

References 37 publications

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“…A large number of phenolic compounds including flavonoids, stilbenoids, and 2-arylbenzofurons have been isolated from Artocarpus plants and possess a wide range of biological properties such as anti-inflammatory (Hsu et al, 2012;Lin et al, 2011), antiplatelet (Jagtap & Bapat, 2010;Weng et al, 2006), antimycobacterial (Loizzo et al, 2010;Siritapetawee et al, 2012), and antiprotozoal activities (Bourjot et al, 2010). Among these activities, the antioxidant actions of these polyphenols derived from Artocarpus species have been frequently reported in vivo and in vitro (Omar et al, 2011;Sidahmed et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of phenolic compounds fromArtocarpus styracifoliuson respiratory burst of rat neutrophils

Ren

Xiang²,

Hu³

et al. 2014

Pharmaceutical Biology

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Materials and methods:The anti-respiratory burst activities of eight phenolics (20 mM) were assessed by determining luminol-dependent chemiluminiscence in rat PMNs. Cytotoxicity of active compounds (1-1000 mM) was assayed by Trypan blue dye exclusion method. Cell-free models were employed to evaluate scavenging capacity of active compounds (20 mM) against reactive oxygen species.Results: The PMA-induced respiratory burst was significantly inhibited (p50.05) by six isoprenylated phenolics (AS1-6) at the concentration of 20 mM (below the toxic concentration) with the inhibition rate ranging from 25.0 to 99.6%. The inhibitory potency estimated by IC 50 was in the order of AS1 (3.1 mM) 4AS6 (5.9 mM) 4AS2 (9.1 mM) 4AS3 (10.0 mM) 4AS5 (29.7 mM) 4AS4 (57.7 mM). AS1-4, four isoprenylated flavones, potently quenched superoxide anion, hydroxyl radical, and hydrogen peroxide at the concentration of 20 mM with their scavenging rates in the range of 30.1-78.1%, 35.4-69.7%, and 65.5-86.3%, respectively. In contrast, AS5-6, two isoprenylated 2-arylbenzofurans, showed less effect than that exhibited by AS1-4. Conclusion and discussion: The isoprenylated phenolics from A. styracifolius can potently inhibit PMA-induced respiratory burst in rat neutrophils without showing cytotoxicity. The inhibitory effects of these isoprenylated phenolics on the respiratory burst might depend on their different types of structure.

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Section: Discussionmentioning

confidence: 99%

Inhibitory effects of phenolic compounds fromArtocarpus styracifoliuson respiratory burst of rat neutrophils

Ren

Xiang²,

Hu³

et al. 2014

Pharmaceutical Biology

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“…The size of jackfruit seeds is extremely large. There are ∼100-500 seeds in the fruit representing 8-15% of the total fruit weight [22,23].…”

Section: Discussionmentioning

confidence: 99%

Purification and characterization of a trypsin inhibitor from the seeds of <italic>Artocarpus heterophyllus</italic> Lam.

Lyu

Liu

et al. 2015

ABBS

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A proteinaceous inhibitor against trypsin was isolated from the seeds of Artocarpus heterophyllus Lam. by successive ammonium sulfate precipitation, ion-exchange, and gel-filtration chromatography. The trypsin inhibitor, named as AHLTI (A. heterophyllus Lam. trypsin inhibitor), consisted of a single polypeptide chain with a molecular weight of 28.5 kDa, which was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel-filtration chromatography. The N-terminal sequence of AHLTI was DEPPSELDAS, which showed no similarity to other known trypsin inhibitor sequence. AHLTI completely inhibited bovine trypsin at a molar ratio of 1:2 (AHLTI:trypsin) analyzed by native polyacrylamide gel electrophoresis, inhibition activity assay, and gel-filtration chromatography. Moreover, kinetic enzymatic studies were carried out to understand the inhibition mechanism of AHLTI against trypsin. Results showed that AHLTI was a competitive inhibitor with an equilibrium dissociation constant (K i ) of 3.7 × 10 −8 M. However, AHLTI showed weak inhibitory activity toward chymotrypsin and elastase. AHLTI was stable over a broad range of pH 4-8 and temperature 20-80°C. The reduction agent, dithiothreitol, had no obvious effect on AHLTI. The trypsin inhibition assays of AHLTI toward digestive enzymes from insect pest guts in vitro demonstrated that AHLTI was effective against enzymes from Locusta migratoria manilensis (Meyen). These results suggested that AHLTI might be a novel trypsin inhibitor from A. heterophyllus Lam. belonging to Kunitz family, and play an important role in protecting from insect pest.

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“…Food-borne illnesses associated with Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157:H7 and Salmonella enteritidis present a major public health concern [2,3]. It has been reported that the incidence of food-borne diseases caused by microbial contamination and environmental pollution will globally increase [4,5]. Furthermore, the consumption of foods contaminated with pathogenic microorganisms, such as bacteria, mould, viruses and parasites active the growth of a pathogen.…”

Section: Introductionmentioning

confidence: 99%

Essential oil of Thymus vulgaris L. and Rosmarinus officinalis L.: Gas chromatography-mass spectrometry analysis, cytotoxicity and antioxidant properties and antibacterial activities against foodborne pathogens

Miladi¹,

Slama²,

Mili³

et al. 2013

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The essential oil composition of Thymus vulgaris L. and Rosmarinus officinalis L. endemic to France were determined by GC and GC-MS. Oils were assessed for their cytotoxic, antioxidant and antimicrobial activity. 31 and 37 different compounds were identified representing 99.64% and 99.38% of the thyme and rosemary oils respectively, where oxygenated monoterpenes constituted the main chemical class. Thymol (41.33%) and 1.8-cineole (24.10%) were identified as the main constituents of T. vulgaris L. and R. officinalis L., respectively. Essential oils (EOs) of selected plant species were evaluated for their in vitro cytotoxicity against the human lung adenocarcinoma epithelial cell line (A549). Cytotoxicity was measured using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphynyltetrazolium bromide] colorimetric assay. Dose-dependent studies revealed IC 50 of 8.50 ± 0.01 µg/mL and 10.50 ± 0.01 µg/mL after 72 h on the A549 cells for R. officinalis L. and T. vulgaris L., respectively. Antioxidant activity was determined using a quantitative DPPH (1,1-diphenyl-2-picryl hydrazyl) assay. Thymus and rosemary EOs exhibited effective radical scavenging capacity with 50% inhibitory concentration (IC 50 ) of 437 ± 5.46 µg/mL and 189 ± 2.38 µg/mL respectively and therefore acts as a natural antioxidant agent. The antimicrobial activity of these species has also been studied against several foodborne pathogens and food isolated Salmonella spp. including S. enteritidis of significant importance. According to the results, T. vulgaris L. showed higher bactericidal effect than those from R. officinalis L. These results suggest that the essential oil from T. vulgaris L. and R. officinalis L. have potential to be used as a natural cytotoxic, antioxidant and antimicrobial agent in food processing.

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Antioxidant and Antibacterial Activities on Foodborne Pathogens of Artocarpus heterophyllus Lam. (Moraceae) Leaves Extracts

Cited by 78 publications

References 37 publications

Inhibitory effects of phenolic compounds fromArtocarpus styracifoliuson respiratory burst of rat neutrophils

Inhibitory effects of phenolic compounds fromArtocarpus styracifoliuson respiratory burst of rat neutrophils

Purification and characterization of a trypsin inhibitor from the seeds of <italic>Artocarpus heterophyllus</italic> Lam.

Essential oil of <i>Thymus vulgaris</i> L. and <i>Rosmarinus officinalis</i> L.: Gas chromatography-mass spectrometry analysis, cytotoxicity and antioxidant properties and antibacterial activities against foodborne pathogens

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Antioxidant and Antibacterial Activities on Foodborne Pathogens of Artocarpus heterophyllus Lam. (Moraceae) Leaves Extracts

Cited by 78 publications

References 37 publications

Inhibitory effects of phenolic compounds fromArtocarpus styracifoliuson respiratory burst of rat neutrophils

Inhibitory effects of phenolic compounds fromArtocarpus styracifoliuson respiratory burst of rat neutrophils

Purification and characterization of a trypsin inhibitor from the seeds of &lt;italic&gt;Artocarpus heterophyllus&lt;/italic&gt; Lam.

Essential oil of &lt;i&gt;Thymus vulgaris&lt;/i&gt; L. and &lt;i&gt;Rosmarinus officinalis&lt;/i&gt; L.: Gas chromatography-mass spectrometry analysis, cytotoxicity and antioxidant properties and antibacterial activities against foodborne pathogens

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Purification and characterization of a trypsin inhibitor from the seeds of <italic>Artocarpus heterophyllus</italic> Lam.

Essential oil of <i>Thymus vulgaris</i> L. and <i>Rosmarinus officinalis</i> L.: Gas chromatography-mass spectrometry analysis, cytotoxicity and antioxidant properties and antibacterial activities against foodborne pathogens