Antioxidant and antibacterial activity of Ipomoea mauritiana Jacq.: a traditionally used medicinal plant in Bangladesh

Abstract

Background In an attempt to explore the scientific basis for the pharmacological benefits the antioxidant and antimicrobial activities of Ipomoea mauritiana whole plant methanol extract were assessed. Methods The total phenolic and flavonoid content were determined using standard method while antioxidant activity was determined by DPPH free radical scavenging activity method. The antimicrobial activity was evaluated by disk diffusion method and compared with standard kanamycin (30 μg/disc). Results The results revealed that I. mauritiana extract contains tannin, saponin, terpenoids, alkaloid and flavonoids. In DPPH, ascorbic acid and extract showed highest scavenging activity and it was 90.96% at concentration 800 μg/mL and I. mauritiana methanol extract showed 72.28% at a concentration of 800 μg/mL. The extract was able to reduce the stable free radical DPPH with an IC50 of 275.084 μg/mL while that of ascorbic acid was 230.09 μg/mL. Total phenolic constituent of the extract was 59.302 ± 3.289 mg/g as gallic acid equivalent. The flavonoid content of methanolic extract of I. mauritiana was 27.212 mg of QE/g. In case of antimicrobial screening, crude extracts of I. mauritiana showed notable antibacterial activity against tested microorganisms. The extract and standard showed the highest mean zone of inhibition ranging from 13 to 19 mm and 37 to 42 mm, respectively at a concentration of 400 μg /disc and against the gram positive bacteria (Bacillus cereus-19.25 mm) showed highest zone of inhibition. Conclusions The results indicate that I. mauritiana possesses considerable antioxidant and antimicrobial activity.

Phytochemical and pharmacological activities of methanol extract of Artemisia vulgaris L. leaves

“…The IC 50 value interprets the results of the DPPH assay, which defies the minimum concentration of methanol extract, requires to scavenging 50% of free radicals [37]. The scavenging properties may be due to chelation and transfer of electron or the ability of hydroxyl groups to donate the hydrogen to free radical [38,39]; similarly the present finding was showed resemblance with these previous studies. Hence it can say that the tested extract have ability to transform the ferric ion (Fe3+) to ferrous ion (Fe2+) as reducing power with the increase of concentration of the extract.…”

Qualitative dataset on UPLC-QTOF/MS tentative identification of phytochemicals from bioactive extract of Ipomoea mauritiana Jacq.

“… Data presented here can be utilized to identify natural flavonoids, saponins, alkaloid and terpenoids by UPLC-ESI-QTOF/MS. IM methanol extract has been reported to show antioxidant and anti-microbial activity against varieties of bacteria and fungi in our previous study [1] . It is very important to know the type of compounds responsible for the activity of this extract.…”

“…IM methanol extract has been reported to show antioxidant and anti-microbial activity against varieties of bacteria and fungi in our previous study [1] . It is very important to know the type of compounds responsible for the activity of this extract.…”

“…The dry plant material were passed through a grinder to ground into a fine powder (Miyako 3 in one blender, Model No: DL-718, China). As previously mentioned [1] , simple maceration technique was applied to prepare the extract. About 50 g of powder was taken in amber glass container and 500 mL methanol was added to soak the powder completely.…”

In-vitro anti-bacterial activity of medicinal plants against Urinary Tract Infection (UTI) causing bacteria along with their synergistic effects with commercially available antibiotics

“…It is estimated that there are about 5000 plant species, of which around 1500 species are expected to have therapeutic benefits [ 8 ]. Over the last decade, numerous studies have been conducted in Bangladesh to analyze the antibacterial properties of eclectic plants like Azadirachta indica (Neem) [ [9] , [10] , [11] ], Rosa kordesii [ 12 ], Hibiscus rosa-sinensis (China rose) [ 13 , 14 ], Curcuma longa (Turmeric) [ 14 , 15 ], Zingiber officinale (Ginger) [ 15 , 16 ], Allium sativum (Garlic) [ 15 ], Piper betle (Betel leaf) [ 17 , 18 ], Launaea sarmentosa [ 19 ], Ficus racemosa (Fig) [ 20 ], Nigella sativa (Black cumin) [ 18 ], Cinnamomum cassia (Chinese cinnamon) [ 21 ], Ocimum tenuiflorum (Tulsi) [ 10 ], Melastoma malabathricum [ 22 ], Polygonum hydropiper L. (Water pepper) [ 23 ], Ipomoea mauritiana (Morning glory) [ 24 ], Eclipta prostrata [ 11 , 22 ], Tagetes minuta (Marigold) [ 9 , 14 ] and so on. A more diverse range of medicinal plants needs to be analyzed extensively to enhance our knowledge further.…”