Antioxidant and Antibacterial Activity of Nepeta × faassenii Bergmans ex Stearn Essential Oil

Jianu, Călin; Moleriu, Radu Dumitru; Stoin, Daniela; Cocan, Ileana; Bujancă, G.; Pop, Georgeta; Lukinich-Gruia, Alexandra Teodora; Muntean, Delia; Rusu, Laura-Cristina; Horhat, Delia Ioana

doi:10.3390/app11010442

Cited by 14 publications

(13 citation statements)

References 42 publications

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“…In this regard, the EOs of different species of Nepeta has also been studied for their DPPH radical scavenging activity and their ability has been proven. The strong activity of the N. mahanesis EO agrees with the reported antioxidant activity of N. faassenii EO [ 48 ]. It is worth mentioning that the IC 50 value obtained in this study is lower than those reported for the EO of N. schiraziana (IC 50 = 52.24 µg/mL) [ 35 ] and N. flavida (IC 50 = 42.8 µg/mL) [ 49 ], indicating the stronger radical scavenging activity of N. mahanesis .…”

Section: Discussionsupporting

confidence: 87%

Chemical composition, anticancer and antibacterial activity of Nepeta mahanensis essential oil

Amirzadeh

Soltanian

Mohamadi

2022

BMC Complement Med Ther

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Background Conventional cancer treatments, such as chemotherapy, radiation therapy, and surgery, often affect the patients’ quality of life due to their serious side effects, indicating the urgent need to develop less toxic and more effective alternative treatments. Medicinal plants and their derivatives are invaluable sources for such remedies. The present study aimed to determine the chemical composition, anticancer and antibacterial activities of Nepeta mahanesis essential oil (EO). Methods The chemical composition of EO was analyzed by gas chromatography-mass spectrometry (GC-MS). Cytotoxicity and apoptosis/necrosis induction of EO was analyzed by MTT assay and Flow cytometry. Real-time PCR was performed to evaluate the Bax/Bcl2 gene expression. Also, the effect of the EO on the cells’ mitochondrial membrane potential (MMP) and ROS level was assessed. DPPH assay was done to assess the free radical scavenging activity of the EO. The Antimicrobial activity, MIC, and MBC of the oil were determined via well-diffusion and broth microdilution methods. Results Based on the GC-MS analysis, 24 compounds were identified in the EO, of which 1,8-cineole (28.5%), Nepetalactone (18.8%), germacrene D (8.1%), and β-pinene (7.2%), were the major compounds. Also, the EO showed considerable cytotoxicity against MCF-7, Caco-2, SH-SY5Y, and HepG2 after 24 and 48 h treatment with IC50 values between 0.0.47 to 0.81 mg/mL. It was revealed that this compound increased the Bax/Bcl2 ratio in the MCF-7 cells and induced apoptosis (27%) and necrosis (18%) in the cells. Moreover, the EO treatment led to a substantial decrease in MMP, which is indicative of apoptosis induction. A significant increase in ROS level was also detected in the cells following exposure to the EO. This compound showed strong DPPH radical scavenging activity (IC50: 30). It was also effective against Gram-positive E. faecalis (ATCC 29,212) and Gram-negative E. coli (ATCC 11,333) bacteria. Conclusions The results of this study demonstrated that the EO of N. mahanesis could be considered a bioactive product with biomedical applications that can be used as an alternative cancer treatment and applied in the biomedical industries.

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Section: Discussionsupporting

confidence: 87%

Chemical composition, anticancer and antibacterial activity of Nepeta mahanensis essential oil

Amirzadeh

Soltanian

Mohamadi

2022

BMC Complement Med Ther

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“…The latter one is a characteristic constituent of this species. A previous study reported that this compound was a major one in the leaf EO of the same species [ 36 , 37 ].…”

Section: Resultsmentioning

confidence: 99%

Valorization of a Waste Product of Edible Flowers: Volatile Characterization of Leaves

et al. 2022

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(1) Background: The leaves of some plants are reported for their culinary uses, while in edible flowers, they are one of the discarded products in the supply chain. We investigated the volatile profile (VP) and the essential oil (EO) compositions of leaves from 12 Lamiaceae species, of which nine belong to the Mentheae tribe and three to the Ocimeae tribe. (2) Methods: Phytochemical analyses were performed using a GC-MS instrument. (3) Results: More than 53% of the Ocimeae tribe VP was represented by sesquiterpene hydrocarbons (SH), followed by phenylpropanoids, except for O. × citriodorum, where oxygenated monoterpenes (OM) were the second main class. OM prevailed in six species of the Mentheae tribe except for Agastache ‘Arcado Pink’, Salvia discolor, and S. microphylla, where SH dominated. The EO composition of Ocimeae tribe showed a similar behavior to that of VP concerning the predominant classes. O. basilicum ‘Blue Spice’ (Ob-BS) was an exception, since it showed oxygenated sesquiterpenes (OS: 29.6%) as a second principal class. Sesquiterpene compounds were also present in a high amount in two species of the Salviinae subtribe (S. microphylla and S. discolor) and two of the Nepetinae subtribe (Nepeta × faasenii and A. ‘Arcado Pink’). The remaining species of the Mentheae tribe were characterized by OM. (4) Conclusions: Many of the main compounds found were reported for their importance in human health and thus are important as ingredients in several new industrial products.

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“…The method described by Danciu et al and Jianu et al [ 38 , 41 ], with minor modifications, was employed to determine the MBC and MFC. Up to 1 µL from each test tube with no visible growth was inoculated on Columbia agar supplemented with 5% sheep blood and incubated for 24 h at 37 °C for bacterial strains or on Sabouraud Dextrose agar supplemented with chloramphenicol and incubated at 28 °C for Candida strains.…”

Section: Methodsmentioning

confidence: 99%

In Vitro and In Silico Evaluation of the Antimicrobial and Antioxidant Potential of Thymus pulegioides Essential Oil

Jianu¹,

Rusu

Muntean

et al. 2022

Antioxidants

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The study was designed to analyze and evaluate the antioxidant and antibacterial properties of the essential oils of Thymus pulegioides L. grown in Western Romania. Thymus pulegioides L. essential oil (TPEO) was extracted by steam distillation (0.71% v/w) using a Craveiro-type apparatus. GC-MS investigation of the TPEO identified 39 different compounds, representing 98.46% of total oil. Findings revealed that thymol (22.89%) is the main compound of TPEO, followed by para-cymene (14.57%), thymol methyl ether (11.19%), isothymol methyl ether (10.45%), and beta-bisabolene (9.53%). The oil exhibits good antibacterial effects; C. parapsilosis, C. albicans, S. pyogenes, and S. aureus were the most sensitive strains. The antioxidant activity of TPEO was evaluated by peroxide and thiobarbituric acid value, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), [2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium] (ABTS) radical scavenging assay, and beta-carotene/linoleic acid bleaching testing. The antioxidative data recorded reveal, for the first time, that TPEO inhibits primary and secondary oxidation products, in some particular conditions, better than butylated hydroxyanisole (BHA) with significant statistical difference (p < 0.05). Moreover, TPEO antioxidant capabilities in DPPH and ABTS assays outperformed alpha-tocopherol (p < 0.001) and delta-tocopherol (p < 0.001). Molecular docking analysis revealed that one potential target correlated with the TPEO antimicrobial activity was d-alanine-d-alanine ligase (DDl). The best scoring ligand, linalyl anthranilate, shared highly similar binding patterns with the DDl native inhibitor. Furthermore, molecular docking analysis also showed that the main constituents of TPEO are good candidates for xanthine oxidase and lipoxygenase inhibition, making the essential oil a valuable source for protein-targeted antioxidant compounds. Consequently, TPEO may represent a new potential source of antioxidant and antibacterial agents with applicability in the food and pharmaceutic industries.

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Antioxidant and Antibacterial Activity of Nepeta × faassenii Bergmans ex Stearn Essential Oil

Cited by 14 publications

References 42 publications

Chemical composition, anticancer and antibacterial activity of Nepeta mahanensis essential oil

Chemical composition, anticancer and antibacterial activity of Nepeta mahanensis essential oil

Valorization of a Waste Product of Edible Flowers: Volatile Characterization of Leaves

In Vitro and In Silico Evaluation of the Antimicrobial and Antioxidant Potential of Thymus pulegioides Essential Oil

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