Antioxidant and antibacterial insights into the leaves, leaf tea and medicinal roots from Astragalus membranaceus (Fisch.) Bge.

Samuel, Anim Okyere; Huang, Baoquan; Chen, Yuan; Guo, Fengjing; Yang, Dou-Dou; Jin, Jianqin

doi:10.1038/s41598-021-97109-6

Cited by 18 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extensive research studies have identified the antioxidant properties of A. membranaceus Fisch. ex Bunge ( Durazzo et al, 2021 ; Samuel et al, 2021 ), C. cassia L.) J. Presl ( Yang et al, 2012 ; Rao and Gan, 2014 ), and S. cuneata (Oliv.) Rehder and E.H. Wilson ( Zhang et al, 2021 ), displaying significant prevention from tissue injury via antioxidant mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…Rehder and E.H. Wilson, and Cinnamomum cassia (L.) J. Presl ( Table 1 ). Interestingly, antioxidant activity is common among all of these components ( Yang et al, 2012 ; Rao and Gan, 2014 ; Durazzo et al, 2021 ; Li et al, 2021 ; Samuel et al, 2021 ; Zhang et al, 2021 ; Xu et al, 2022 ). According to TCM theory, the combination of these five drugs work in concert to reduce inflammation, resist oxidation, and fight against aging by enriching Qi, eliminating phlegm and blood stasis, and promoting blood circulation; thereby suitable for the treatment of stroke, atherosclerosis, and other related cardiovascular and cerebrovascular conditions ( Liang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Zhilong Huoxue Tongyu capsule attenuates intracerebral hemorrhage induced redox imbalance by modulation of Nrf2 signaling pathway

Mazhar,

Yang,

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

Background: One of the severely debilitating and fatal subtypes of hemorrhagic stroke is intracerebral hemorrhage (ICH), which lacks an adequate cure at present. The Zhilong Huoxue Tongyu (ZLHXTY) capsule has been utilized effectively since last decade to treat ICH, in some provinces of China but the scientific basis for its mechanism is lacking. Purpose: To investigate the neuroprotective role of ZLHXTY capsules for ICH-induced oxidative injury through the regulation of redox imbalance with the Nrf2 signaling pathway.Methods: Autologous blood injection model of ICH in C57BL/6J mice was employed. Three treatment groups received ZLHXTY once daily through oral gavage at doses 0.35 g/kg, 0.7 g/kg, and 1.4 g/kg, started after 2 h and continued for 72 h of ICH induction. The neurological outcome was measured using a balance beam test. Serum was tested for inflammatory markers IL-1β, IL-6, and TNF-α through ELISA, oxidative stress through hydrogen peroxide content assay, and antioxidant status by total antioxidant capacity (T-AOC) assay. Nuclear extract from brain tissue was assayed for Nrf2 transcriptional factor activity. RT-qPCR was performed for Nfe2l2, Sod1, Hmox1, Nqo1, and Mgst1; and Western blotting for determination of protein expression of Nrf2, p62, Pp62, Keap, HO1, and NQO1. Fluoro-jade C staining was also used to examine neuronal damage.Results: ZLHXTY capsule treatment following ICH demonstrated a protective effect against oxidative brain injury. Neurological scoring showed improvement in behavioral outcomes. ELISA-based identification demonstrated a significant decline in the expression of serum inflammatory markers. Hydrogen peroxide content in serum was found to be reduced. The total antioxidant capacity was also reduced in serum, but the ZLHXTY extract showed a concentration-dependent increase in T-AOC speculating at its intrinsic antioxidant potential. Nrf2 transcriptional factor activity, mRNA and protein expression analyses revealed normalization of Nrf2 and its downstream targets, which were previously elevated as a result of oxidative stress induced by ICH. Neuronal damage was also reduced markedly after ZLHXTY treatment as revealed by Fluoro-jade C staining. Conclusion: ZLHXTY capsules possess an intrinsic antioxidant potential that can modulate the ICH-induced redox imbalance in the brain as revealed by the normalization of Nrf2 and its downstream antioxidant targets.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Zhilong Huoxue Tongyu capsule attenuates intracerebral hemorrhage induced redox imbalance by modulation of Nrf2 signaling pathway

Mazhar,

Yang,

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Several randomized clinical trials have demonstrated that Astragalus-based interventions can reduce cancer symptoms, improve the quality of life and immune function, increase plasma nerve growth factor levels, and slow the progression of chemotherapyinduced peripheral neuropathy [78,156]. Currently, Astragalus extracts or preparations [157] have been used in clinical trials for their antitumor effects on digestive, respiratory, epithelial tissue, and hematological malignancies (Table 3) [158][159][160].…”

Section: Clinical Trials On the Antitumor Effects Of Apsmentioning

confidence: 99%

Anticancer Mechanism of Astragalus Polysaccharide and Its Application in Cancer Immunotherapy

He,

Liu,

Qin

et al. 2024

Pharmaceuticals

View full text Add to dashboard Cite

Astragalus polysaccharide (APS) derived from A. membranaceus plays a crucial role in traditional Chinese medicine. These polysaccharides have shown antitumor effects and are considered safe. Thus, they have become increasingly important in cancer immunotherapy. APS can limit the spread of cancer by influencing immune cells, promoting cell death, triggering cancer cell autophagy, and impacting the tumor microenvironment. When used in combination with other therapies, APS can enhance treatment outcomes and reduce toxicity and side effects. APS combined with immune checkpoint inhibitors, relay cellular immunotherapy, and cancer vaccines have broadened the application of cancer immunotherapy and enhanced treatment effectiveness. By summarizing the research on APS in cancer immunotherapy over the past two decades, this review elaborates on the anticancer mechanism of APS and its use in cancer immunotherapy and clinical trials. Considering the multiple roles of APS, this review emphasizes the importance of using APS as an adjunct to cancer immunotherapy and compares other polysaccharides with APS. This discussion provides insights into the specific mechanism of action of APS, reveals the molecular targets of APS for developing effective clinical strategies, and highlights the wide application of APS in clinical cancer therapy in the future.

show abstract

“…It was shown that neither caffeic acid nor rosmarinic acid are present in the A. mongholicus raw material. Recent research indicates that the antiradical capacity of these raw material root extracts is attributed to the presence of polysaccharides and flavonoids [31][32][33][34].…”

Section: Astragalus Mongholicusmentioning

confidence: 99%

Determining Antiradical Capacity of Medicinal Plant Extract Individual Constituents Using Post-Column Reaction Method

Przybył,

Stefaniak,

Jaroszewicz

et al. 2024

IJMS

View full text Add to dashboard Cite

The post-column reaction method enables the evaluation of the antiradical capacity of individual components in a mixture by separating the components using HPLC and measuring stable free radical (e.g., DPPH●) scavenging that occurs after the chromatography column. The equipment typically consists of two detectors. The first records signals of the analytes leaving the column. The second records radical scavenging by the analytes, which appears as a negative band. The recorded signals are found on two separate chromatograms, which must be combined to interpret the results. In this study, a single DAD detector was used behind the post-column reactor, enabling the simultaneous recording of the analyte bands and negative signals, indicating radical scavenging. The objective of this study was to evaluate the antiradical capacity of key compounds found in two herbal raw materials used in traditional Chinese medicine. Saposhnikovia divaricata roots contain phenolic acids, chromones, and furanocoumarins. Chlorogenic acid, rosmarinic acid, and imperatorin demonstrated strong radical scavenging, while prim-O-glucoslocimifugin showed a weaker response, both in standards and in root extracts. However, scavenging was not observed for cimifugin and 4′-O-β-D-glucosyl-5-O-methylvisamminol. Astragalus mongholicus roots contain astragalosides I-IV (triterpene saponins). None of these showed DPPH● scavenging. Furthermore, additional signals were observed, indicating the presence of unidentified radical scavenging compounds.

show abstract

Antioxidant and antibacterial insights into the leaves, leaf tea and medicinal roots from Astragalus membranaceus (Fisch.) Bge.

Cited by 18 publications

References 26 publications

Zhilong Huoxue Tongyu capsule attenuates intracerebral hemorrhage induced redox imbalance by modulation of Nrf2 signaling pathway

Zhilong Huoxue Tongyu capsule attenuates intracerebral hemorrhage induced redox imbalance by modulation of Nrf2 signaling pathway

Anticancer Mechanism of Astragalus Polysaccharide and Its Application in Cancer Immunotherapy

Determining Antiradical Capacity of Medicinal Plant Extract Individual Constituents Using Post-Column Reaction Method

Contact Info

Product

Resources

About