Antioxidant and Antibacterial Profiling of Pomegranate-pericarp Extract Functionalized-zinc Oxide Nanocomposite

Abstract

With the advancement in green nanotechnology, considerable attention is being given to the synthesis of different kinds of nanomaterials for biological applications. In this study, zinc oxide nanocomposites (ZnO NPs) were synthesized using Punica granatum L. (Pomegranate) pericarp ethanolic extract (PE) by the chemical precipitation method. The prepared ZnO NPs showed a characteristic peak at 270 nm in the UV-Vis spectrophotometer and chemical bond stretching in the Fourier transforms infrared spectroscopy (FT-IR) spectra, indicated the formation of PE-functionalized zinc oxide nanocomposite (PE-ZnO NPs). The SEM results showed agglomerated PE-ZnO NPs of a spherical shape with an average size of 80–100 nm. Moreover, biological assessment of the PE-ZnO NPs revealed significant scavenging activity in DPPH (116.5%) and ABTS ·+ (95.2%) radical assay methods, and substantial antibacterial activity against Bacillus cereus, Bacillus licheniformis , and Escherichia coli . Furthermore, PE-ZnO NPs showed about 96.3% of cell viability for human HaCaT cells at the maximum concentration (100 µg/mL), marked as a reliable bioactive agent. Therefore, the developed PE-ZnO NPs were elucidated with substantial ROS scavenger and non-antibiotic antibacterial agent and hence, can be applied in respective biological applications.

Anti-Inflammatory, Antioxidative, and Nitric Oxide-Scavenging Activities of a Quercetin Nanosuspension with Polyethylene Glycol in LPS-Induced RAW 264.7 Macrophages

“…B. cereus is a facultative, anaerobic, gram-positive, and spore-forming soil, plant, and food bacterium, which has been associated with the development of foodborne diseases, including severe nausea, vomiting, and diarrhea [ 86 ]. Additionally, E. coli is a facultative, anaerobic, gram-negative, rod-shaped, coliform bacteria typically detected in mammals’ gastrointestinal tract [ 87 ]. While most E. coli strains are not harmful to humans, others, such as E. coli O157:H7 has been linked to severe food poisoning [ 88 ].…”

“…The DPPH˙ free-radical-scavenging activity with Qu and Qu-PEG NS was evaluated in contrast to ascorbic acid [ 87 ]. In brief, 200 µL of the 10, 50, 100, 250, 500, and 1000 µg/mL NQS1 solution and 100 µL of the 0.2 mM DPPH solution in ethanol were combined.…”

Establishment of a Sonotrode Extraction Method and Evaluation of the Antioxidant, Antimicrobial and Anticancer Potential of an Optimized Vaccinium myrtillus L. Leaves Extract as Functional Ingredient

“…Polyphenols, such as chlorogenic acid and 3,5-dicaffeoylquinic acid, which are present in bilberry leaves, have demonstrated antiproliferative, anti-inflammatory and antidiabetic activities in numerous studies due to their ability to interfere with proinflammatory cytokines synthesis, immune cell regulation and gene expression [ 9 , 10 ]. Moreover, many phenolic compounds possess significant antimicrobial activity, which may be related to their lipophilia and their interaction with the microbial cell surfaces [ 11 , 12 , 13 ].…”

Facile Green Synthesis of Zinc Oxide Nanoparticles with Potential Synergistic Activity with Common Antifungal Agents against Multidrug-Resistant Candidal Strains

“…Punica granatum L. (pomegranate) fruit belongs to the Punicaceae family and is eminent based on its phytochemical constituents of phenolic acids, tannins and flavonoids, which have significant nutritional properties [33]. Pomegranate-pericarp aqueous extract functionalized green synthesis of ZnO nanoparticles with potential antibacterial potency against Bacillus licheniformis, Bacillus cereus and Escherichia coli strains [34]. Furthermore, the antibacterial effectiveness of ZnO NPs synthesized using pomegranate extracts was reported against several bacterial pathogens, such as Klebsiella pneumoniae, Staphylococcus aureus, Salmonella typhi, Bacillus cereus, Pseudomonas aeruginosa, Streptococcus pneumoniae and Aeromonas hydrophila [35].…”