Antioxidant and Antibacterial Properties of a Functional Sports Beverage Formulation

Kolonas, Alexandros; Vareltzis, Patroklos; Kiroglou, Smaro; Goutzourelas, Nikolaos; Stagos, Dimitrios; Trachana, Varvara; Tsadila, Christina; Mossialos, Dimitris; Mourtakos, Stamatis; Gortzi, Olga

doi:10.3390/ijms24043558

Cited by 5 publications

(2 citation statements)

References 99 publications

(144 reference statements)

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“…GSH levels were determined according to Kolonas and colleagues [81]. Briefly, 50 µL of cell lysate was mixed with 67 mM of potassium phosphate buffer (pH 8) and freshly made DTNB [5,5 ′ -dithiobis-2 nitrobenzoate] dissolved in 1 mM in 1% sodium citrate and vortexed.…”

Section: Determination Of Gsh Levelsmentioning

confidence: 99%

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Cryptomphalus aspersa Egg Extract Protects against Human Stem Cell Stress-Induced Premature Senescence

Outskouni,

Christodoulou,

Goutas

et al. 2024

IJMS

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Cellular senescence is a tightly regulated pathophysiologic process and is caused by replicative exhaustion or external stressors. Since naturally derived bioactive compounds with anti-ageing properties have recently captured scientific interest, we analysed the anti-ageing and antioxidant efficacy of Cryptomphalus aspersa egg extract (CAEE). Its effects on stemness, wound-healing properties, antioxidant defense mechanisms, and DNA damage repair ability of Human Wharton’s jelly mesenchymal stem cells (WJ-MSCs) were analysed. Our results revealed that CAEE fortifies WJ-MSCs stemness, which possibly ameliorates their wound-healing ability. Additionally, we show that CAEE possesses a strong antioxidant capacity as demonstrated by the elevation of the levels of the basic antioxidant molecule, GSH, and the induction of the NRF2, a major antioxidant regulator. In addition, CAEE alleviated cells’ oxidative stress and therefore prevented stress-induced premature senescence (SIPS). Furthermore, we demonstrated that the prevention of SIPS could be mediated via the extract’s ability to induce autophagy, as indicated by the elevation of the protein levels of all basic autophagic molecules and the increase in formation of autophagolysosomes in CAEE-treated WJ-MSCs. Moreover, CAEE-treated cells exhibited decreased Caveolin-1 levels. We propose that Cryptomphalus aspersa egg extract comprises bioactive compounds that can demonstrate strong antioxidant/anti-ageing effects by regulating the Caveolin-1–autophagy–senescence molecular axis.

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Section: Determination Of Gsh Levelsmentioning

confidence: 99%

“…TBARS assay used here was described by Kolonas and colleagues [81]. All initial steps of the procedure are the same as in Section 4.8.…”

Section: Tbars Assay For the Determination Of Lipid Peroxidationmentioning

confidence: 99%

Cryptomphalus aspersa Egg Extract Protects against Human Stem Cell Stress-Induced Premature Senescence

Outskouni,

Christodoulou,

Goutas

et al. 2024

IJMS

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Hypnea musciformis Seaweed Extract Protected Human Mesenchymal Stem Cells From Oxidative Stress Through NRF2 Activation

Goutas,

Goutzourelas,

Kevrekidou

et al. 2024

Food Science & Nutrition

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Previous studies have shown that Hypnea musciformis seaweed extracts (HMEs) possess antioxidant properties, but the molecular mechanisms accounting for this activity are not known. Thus, the present study investigated the molecular mechanisms through which HME exerted its antioxidant activity in human mesenchymal stem cells (WJ‐MSCs). After the isolation of HME, its chemical composition was analyzed with gas chromatography mass spectrometry, indicating that it contained amino acids, organic acids, organic amides, sugar alcohols, saturated fatty acids, hydrogenated diterpene alcohols, and other organic compounds. Afterward, HME was shown in vitro to scavenge DPPH·, ABTS·+, ·OH, and O2·− radicals, possess reducing activity, and protect from ROO·‐induced DNA strand breakage. Finally, the results showed that HME treatment of WJ‐MSCs prevented H2O2‐induced oxidative stress by decreasing lipid peroxidation, protein oxidation, reactive oxygen species levels, and DNA damage and by increasing glutathione levels. Moreover, our findings showed for the first time that HME's antioxidant activity in WJ‐MSCs was mediated through the activation of NRF2, which upregulated the expression of the antioxidant proteins GCLC, GSR, HMOX1, SOD1, TXN, and GPX1. These results provide new insights into H. musciformis' antioxidant properties, which could help substantially its use as a food supplement or for developing biofunctional foods.

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A Novel Bioactive Antimicrobial Film Based on Polyvinyl Alcohol-Protocatechuic Acid: Mechanism and Characterization of Biofilm Inhibition and its Application in Pork Preservation

Gao,

Tian,

et al. 2024

Food Bioprocess Technol

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Antioxidant and Antibacterial Properties of a Functional Sports Beverage Formulation

Cited by 5 publications

References 99 publications

Cryptomphalus aspersa Egg Extract Protects against Human Stem Cell Stress-Induced Premature Senescence

Cryptomphalus aspersa Egg Extract Protects against Human Stem Cell Stress-Induced Premature Senescence

Hypnea musciformis Seaweed Extract Protected Human Mesenchymal Stem Cells From Oxidative Stress Through NRF2 Activation

A Novel Bioactive Antimicrobial Film Based on Polyvinyl Alcohol-Protocatechuic Acid: Mechanism and Characterization of Biofilm Inhibition and its Application in Pork Preservation

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