Antioxidant and Anticancer Effects of Epsilon-Poly-L-lysine Produced by Two Novel Strains of Paenibacillus polymyxa HS6 and Stenotrophomonas maltophilia YS8

Ranjbar, Hamidreza Hagh; Hosseini-Abari, Afrouzossadat; Ghasemi, Seyed Mahdi; Ghorbani, Najmeh

doi:10.1007/s12257-022-0065-1

Cited by 5 publications

(3 citation statements)

References 46 publications

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Section: Resultsmentioning

confidence: 99%

“…According to previous studies, ε-PL as a natural antimicrobial peptide can inactivate microorganisms including bacteria, yeast, and viruses [41]. Being cationic allows ε-PL to interact with negatively charged molecules and cell surfaces such as bacterial membranes, viral components, and fungal cell walls [42,43]. Liu et al have shown that interaction and the peptide backbone structure of ε-PL, which is affected by pH, could induce different antibacterial activity.…”

Section: Antibacterial Activity Of ε-Plmentioning

confidence: 99%

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Antibacterial activity of epsilon-poly-l-lysine produced by Stenotrophomonas maltophilia HS4 and Paenibacillus polymyxa HS5, alone and in combination with bacteriophages

Hagh Ranjbar,

Hosseini-Abari,

Ghasemi

et al. 2023

Microbiology

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Over the past decades, antibiotic resistance has become a major clinical problem, and searching for new therapeutic strategies seems to be necessary. Using novel natural compounds, antimicrobial peptides, and bacteriophages is the most promising solution. In this study, various cationic metabolite-producer bacteria were isolated from different soil samples. Two isolates were identified as Stenotrophomonas maltophilia HS4 (accession number: MW791428) and Paenibacillus polymyxa HS5 (accession number: MW791430) based on biochemical characteristics and phylogenetic analysis using 16S rRNA gene sequences. The cationic compound in the fermentation broth was precipitated and purified with sodium tetraphenylborate salt. The purified cationic peptide was confirmed to be epsilon-poly-l-lysine by structural and molecular analysis using High-Performance Liquid Chromatography, Sodium dodecyl-sulfate-polyacrylamide gel electrophoresis, and Fourier-transform infrared spectroscopy. The antibacterial activity of epsilon-poly-l-lysine was evaluated against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Serratia marcescens ATCC 13880, and Klebsiella pneumoniae ATCC 13883 by microdilution method. Furthermore, the antibacterial effects of purified epsilon-poly-l-lysine in combination with two long non-contractile tail bacteriophages against vancomycin-resistant Enterococcus faecalis and colistin-resistant Klebsiella pneumoniae were investigated. The results indicated great antibacterial activity of epsilon-poly-l-lysine which was produced by two novel bacteria. The epsilon-poly-l-lysine as a potent cationic antimicrobial peptide is demonstrated to possess great antimicrobial activity against pathogenic and also antibiotic-resistant bacteria.

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Section: Resultsmentioning

confidence: 99%

Section: Antibacterial Activity Of ε-Plmentioning

confidence: 99%

Antibacterial activity of epsilon-poly-l-lysine produced by Stenotrophomonas maltophilia HS4 and Paenibacillus polymyxa HS5, alone and in combination with bacteriophages

Hagh Ranjbar,

Hosseini-Abari,

Ghasemi

et al. 2023

Microbiology

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“…Recently, it is reported that Ɛ‐PL not only acts as an antimicrobial agent but showed antioxidants and antitumor activity (Ranjbar et al, 2022; Shi et al, 2016). The antioxidant activity of Ɛ‐PL was determined by the 2,2‐diphenyl‐1‐picryl‐hydrazine‐hydrate (DPPH) method showed that the radical scavenging activity of Ɛ‐PL at a minimum concentration (0.18 g/L) was at least 7% which increased to 90% at 8 g/L (Ranjbar et al, 2022). The DPPH free radical is a stable free radical, which has been widely accepted as a tool for estimating the free radical–scavenging activity of antioxidants.…”

Section: Discussionmentioning

confidence: 99%

Epsilon poly‐lysine in buffalo semen extender: A step towards reducing the development of antibiotic resistance

Yadav,

Dutt,

Bansal

et al. 2023

Reprod Domestic Animals

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The use of antibiotics in semen extenders can contribute to the development of antibiotic resistance. The objective of the study was to evaluate epsilon‐polylysine (Ɛ‐PL) as a substitute for antibiotics in the buffalo semen extender. For this, 20 semen ejaculates were collected from four Murrah buffalo bulls. Each ejaculate was divided into three equal aliquots and extended into an egg yolk‐based semen extender containing either antibiotics (strepto‐penicillin) or different concentrations of Ɛ‐PL (0.64 and 1.28 g/L) to make the final concentration 80 million sperm/mL and cryopreserved as per the standard procedure. The antibiogram sensitivity test confirmed that Ɛ‐PL is an effective antimicrobial against microbes present in buffalo semen ejaculates. Furthermore, the addition of Ɛ‐PL in the semen extender significantly reduces the colony forming unit (CFU)/mL in cryopreserved semen equivalent to strepto‐penicillin. The sperm motility and kinematic parameters assessed by a computer‐assisted sperm analyser showed that Ɛ‐PL did not inhibit either sperm motility not kinematic parameters of cryopreserved sperm. The flow‐cytometric evaluation of frozen–thawed sperm revealed interesting results. The extender supplemented with Ɛ‐PL protected sperm acrosome and mitochondrial membrane potential greater than the extender supplemented with strepto‐penicillin. Further, Ɛ‐PL reduced significantly the production of superoxide anions from mitochondria during the cryopreservation process. In this way, Ɛ‐PL may be a suitable alternative to antibiotics in semen extenders. In conclusion, Ɛ‐PL at a concentration of 0.64 g/L acts as an effective antimicrobial as well as antioxidant in semen extender for cryopreservation of buffalo sperm.

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Evaluation of glycyl-arginine and lysyl-aspartic acid dipeptides for their antimicrobial, antibiofilm, and anticancer potentials

Sevim Akan,

Şahal,

Karaca

et al. 2023

Arch Microbiol

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Antioxidant and Anticancer Effects of Epsilon-Poly-L-lysine Produced by Two Novel Strains of Paenibacillus polymyxa HS6 and Stenotrophomonas maltophilia YS8

Cited by 5 publications

References 46 publications

Antibacterial activity of epsilon-poly-l-lysine produced by Stenotrophomonas maltophilia HS4 and Paenibacillus polymyxa HS5, alone and in combination with bacteriophages

Antibacterial activity of epsilon-poly-l-lysine produced by Stenotrophomonas maltophilia HS4 and Paenibacillus polymyxa HS5, alone and in combination with bacteriophages

Epsilon poly‐lysine in buffalo semen extender: A step towards reducing the development of antibiotic resistance

Evaluation of glycyl-arginine and lysyl-aspartic acid dipeptides for their antimicrobial, antibiofilm, and anticancer potentials

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