Antioxidant and Anticarcinogenic Potentials of Propolis for Dimethylhydrazine-Induced Colorectal Cancer in Wistar Rats

Salehi, Alireza; Hosseini, Seyed Mohammad; Kazemi, Sohrab

doi:10.1155/2022/8497562

Cited by 14 publications

(17 citation statements)

References 82 publications

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“…The levels of MDA, SOD, and TAC in all homogenates were measured by employing the TebPazhouhan Razi, Nasdox, and Naxifer kits, respectively [ 41 ]. Regarding each marker, the manual was followed based on the guideline [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

“…Blood and Serum Sampling. The blood samples were collected for examining the CBC (Celltac Es MEK-7300 K, Nihon Kohden) and measuring the related serum markers on a BIOLIS24i autoanalyzer (Tokyo Boeki Medisys Inc) [40].…”

Section: Study Design Vitamin E and Dimethylhydrazine (Dmh)mentioning

confidence: 99%

“…Colon Homogenization. The colon tissue homogenates were utilized by a previously approved method [40]. The homogenates were kept at -80 °C until determining the oxidative stress markers.…”

Section: Study Design Vitamin E and Dimethylhydrazine (Dmh)mentioning

confidence: 99%

“…Western Blot. Similar to the oxidative stress assessment, the homogenates were utilized by the method of a previous study [40]. Further, the primary antibodies of APC, PCNA, CEA, and PDGF (at 4 °C for 12 h) and proper secondary antibodies related to peroxidase conjugate were, respectively, applied to incubate the blots.…”

Section: Oxidative Stress Markersmentioning

confidence: 99%

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Antioxidant and Anticancer Potentials of the Olive and Sesame Mixture against Dimethylhydrazine-Induced Colorectal Cancer in Wistar Rats

Valaei

Azadeh

Niaki

et al. 2022

BioMed Research International

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Cancer is one of the leading causes of death worldwide, and natural agents have shown some promise in fighting it. Thus, the present study tried to evaluate the healing potential of an equal combination of olive and sesame extract (MOS) against the colorectal cancerous lesions that were induced by dimethylhydrazine (DMH) in male rats and also compare the anticarcinogenic potential of the MOS and vitamin E with each other. Therefore, the mixture of equal olive and sesame extract (MOS) was used as the main treatment, alongside vitamin E as a parallel treatment. This study examined the red blood cell (RBC) and white blood cell (WBC) levels, biochemical indices, lactate dehydrogenase (LDH), C-reactive protein (CRP), total protein (TP), creatine kinase (CPK), albumin, and the colon tissue pathology, as well as the level of protein expression of the adenomatous polyposis coli (APC), proliferating cell nuclear antigen (PCNA), carcinoembryonic antigen (CEA), and platelet-derived growth factor (PDGF). Also, the tissue stress markers including total antioxidant capacity (TAC), malondialdehyde (MDA), and superoxide dismutase (SOD) were analyzed. Overall, the results represented a significant reduction in the congestion, mitotic index, inflammation, and cell destruction in the MOS group compared to the DMH group. In terms of the oxidative stress level, a significant increase was observed in the DMH group in comparison with the DMH-MOS group ( P < 0.05 ), and the MOS significantly increased TAC level ( P < 0.05 ). Furthermore, the DMH+MOS-exposed group exhibited a significantly lower expression of the PCNA, CEA, and PDGF proteins than those of the DMH group. Overall, the MOS showed that it can effectively prevent DMH-induced colon lesions. This mixture, as a strong antioxidant agent, can be clinically applied for preventing and treating colorectal cancer, the effectiveness of which is higher than that of vitamin E.

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Section: Methodsmentioning

confidence: 99%

Section: Study Design Vitamin E and Dimethylhydrazine (Dmh)mentioning

confidence: 99%

“…Colon Homogenization. The colon tissue homogenates were utilized by a previously approved method [40]. The homogenates were kept at -80 °C until determining the oxidative stress markers.…”

Section: Study Design Vitamin E and Dimethylhydrazine (Dmh)mentioning

confidence: 99%

Section: Oxidative Stress Markersmentioning

confidence: 99%

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Antioxidant and Anticancer Potentials of the Olive and Sesame Mixture against Dimethylhydrazine-Induced Colorectal Cancer in Wistar Rats

Valaei

Azadeh

Niaki

et al. 2022

BioMed Research International

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“…were purchased and powdered. Next, it was extracted by solving it in absolute ethanol, and then the extract was stored in a refrigerator at 4 °Celsius until it was used [22].…”

Section: Extract Preparation 2 Kg Of Freshly Dried Licorice Rootmentioning

confidence: 99%

Effects of Glycyrrhiza Glabra (Licorice) Root Extract on the Hormones, Serum Biochemicals, and Hematological Parameters in Dogs with Benign Prostatic Hyperplasia

Azadeh

Gharib

Hosseini

2022

Evidence-Based Complementary and Alternative Medicine

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Benign prostatic hyperplasia (BPH) is the most common prostate disorder in non-neutered dogs and is often caused by androgenic stimulation or changes in the ratio of androgen to estrogen. Also, it is commonly seen in neutered older dogs. Clinical signs may not be presented, but persistent or intermittent hematuria, hemospermia, or a primary hemorrhagic discharge are usually reported. In this research, ethanolic extract of licorice root (EELR) was used as the treatment, which has various antioxidant and healing properties and can reduce lesions by establishing an antioxidant balance and reducing inflammation. For this experiment, 30 dogs of approximately the same age and weight were randomly divided into 6 groups of 5 each. The treatment groups received the EELR at doses of 10 and 20 mg/kg separately, while the control group received no medications. At the end of the 9-week treatment period, biochemical and hormonal factors were measured and analyzed by blood sampling. The results showed that the EELR has multiple positive effects on the serum biochemical indices and also positively affects hormone levels, while it can decrease the prostate-specific antigen (PSA) level in BPH dogs. In conclusion, the EELR can effectively reduce BPH-induced lesions without any side effects.

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Propolis ameliorates renal, liver, and pancreatic lesions in Wistar rats

Salehi,

Hosseini,

Kazemi

2024

Biotech and App Biochem

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This study aimed to evaluate the potential of ethanolic extract of propolis on the secondary lesions of the liver, renal, and pancreatic that were derived by primary colorectal cancer, and comparison of the ethanolic extract of propolis with the vitamin E. The groups included the control, ethanolic extract of propolis, vitamin E, dimethylhydrazine, dimethylhydrazine + ethanolic extract of propolis, and dimethylhydrazine + vitamin E. After 13 weeks of treatment, the blood and tissue samples were taken from all the rats, and alanine transaminase, aspartate transaminase, alkaline phosphatase, uric acid, creatinine, blood urea nitrogen, insulin, amylase, and lipase indices along with the tissue pathological examination of the kidney, liver, and pancreas were evaluated. Ethanolic extract of propolis effectively alleviated the colorectal cancer‐induced secondary lesions in the liver by significantly lowering the alanine transaminase significantly. Ethanolic extract of propolis significantly decreased uric acid in rats; and also significantly elevated the pancreatic insulin. In addition, inflammation and cell necrosis indices in all these tissues were significantly reduced when ethanolic extract of propolis was consumed compared to the dimethylhydrazine group. It seemed ethanolic extract of propolis showed high antioxidant, anticancer, and anti‐inflammatory potentials, and can be used practically to reduce the side lesions of colorectal cancer.

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Antioxidant and Anticarcinogenic Potentials of Propolis for Dimethylhydrazine-Induced Colorectal Cancer in Wistar Rats

Cited by 14 publications

References 82 publications

Antioxidant and Anticancer Potentials of the Olive and Sesame Mixture against Dimethylhydrazine-Induced Colorectal Cancer in Wistar Rats

Antioxidant and Anticancer Potentials of the Olive and Sesame Mixture against Dimethylhydrazine-Induced Colorectal Cancer in Wistar Rats

Effects of Glycyrrhiza Glabra (Licorice) Root Extract on the Hormones, Serum Biochemicals, and Hematological Parameters in Dogs with Benign Prostatic Hyperplasia

Propolis ameliorates renal, liver, and pancreatic lesions in Wistar rats

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