Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus

Liu, Xin; Sun, Zilong; Zhang, Miansong; Meng, Xiumei; Xia, Xuekui; Yuan, Wenjie; Xue, Feng; Liu, Changheng

doi:10.1016/j.carbpol.2012.07.047

Cited by 172 publications

(110 citation statements)

References 25 publications

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“…The cholesterol biosynthesis is controlled by the rate limiting enzyme, HMG-CoA reductase. Statin blocks this enzyme and thereby prevents cholesterol biosynthesis [51,62]. In this study, the cholesterol lowering effects of statin (mainly LDL-C) can be attributed to HMG-CoA reductase inhibition.…”

Section: Histopathological Investigationmentioning

confidence: 86%

Statistical Data Analysis Which Result From the Bio-Diagnosis and Bio-Treatment of Injured Rats With the Hyperlipidemia and Hyperglycemia Diseases

Abduallah¹,

Abd‐Alla²,

Ali³

et al. 2016

Asian J Pharm Clin Res

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Objective: This study in bioinformatics aims to investigate the potential effect of Ipomoea tricolor and Sophora tomentosa on liver function enzymes activity, serum lipid profile, oxidative stress biomarkers, and on blood glucose in high fat diet-induced hypercholesterolemia (HC) and STZ-induced hyperglycemia (HG) in rats.Methods: Blood glucose level, liver function enzymes, alanine aminotransferases and aspartate aminotransferases, alkaline phosphatase, and lactate dehydrogenase (LDH) were determined. Besides, lipid profile including total cholesterol (TC), triacylglycerol (TG), total lipid, and high-density lipoprotein-cholesterol was investigated. Moreover, oxidative stress biomarkers, lipid peroxide, and nitric oxide as well as non-enzymatic antioxidant, glutathione (GSH) were also examined in different therapeutic groups.Results: A significant increase in blood glucose level, liver function enzyme activities, LDH, lipid profile and oxidative stress markers, while significant decrease in LDH-C and GSH level in HC-HG induced rats compared to control one. A marked amelioration in all biochemical parameters under investigation on treatment of HC-HG rats with I. tricolor and S. tomentosa with different fluctuating percentages of improvement. Histopathological examination of liver and pancreas was also performed and declared HC-HG showed congestion in portal vessels and sinusoids with mild centrilobular hepatocyte degeneration, marked hepatocyte ballooning and hydropic degeneration, while HC-HG treated rats with I. tricolor and S. tomentosa showed normal lobular hepatic architecture with mild sinusoidal dilatation and congestion. On the other hand, a histological organization of pancreas of HC-HG rats showing disarrangement changes in pancreatic blood vessels and interlobular duct as well as disordered in acini. The treatment of HC-HG rats with I. tricolor and S. tomentosa showed enhancement in Langerhans cells and restore of most pancreatic tissue in comparison with standard drugs. Conclusion:The statistical results showed that each extract ameliorated high blood glucose level liver injury, HC and oxidative stress indicating relieving of oxidative damage associated with the complexity of HG and HC. These results demonstrated that these two plants extracts may be a candidate intelligent antioxidant, hypolipidemic, hypoglycemic, and hepatoprotective nutraceuticals which need further clinical investigation to be applied effectively to reduce perturbation in HC associated diabetes.

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Section: Histopathological Investigationmentioning

confidence: 86%

Statistical Data Analysis Which Result From the Bio-Diagnosis and Bio-Treatment of Injured Rats With the Hyperlipidemia and Hyperglycemia Diseases

Abduallah¹,

Abd‐Alla²,

Ali³

et al. 2016

Asian J Pharm Clin Res

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“…The scavenging rate of undiluted hydrolyzate on hydroxyl radicals was 55.45%, which was equal to the VC solution at 1.0 mg mL −1 . Combined with other study (Liu et al, 2012), it can be concluded that the source of scavenging ability of the hydrolyzate on hydroxyl radicals was from the polysaccharide content of the cucumber. Therefore, the hydrolyzate should be concentrated if we want to obtain the stronger scavenging capacity for hydroxyl radicals.…”

Section: Scavenging Ability Of the Hydrolyzate On Hydroxyl Radicalsmentioning

confidence: 99%

Optimization of Enzymatic Hydrolysis of Freeze-Dried Sea Cucumber Powder with Response Surface Methodology and Its Antioxidant Evaluation

Pang¹,

Sun²,

Liu³

2016

American Journal of Biochemistry and Biotechnology

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In this study, freeze-dried sea cucumber powder was used as raw material and the preparation processing conditions were studied for hydrolysis of sea cucumber powder with neutral protease using Response Surface Methodology (RSM) to obtain a high concentration of sea cucumber polypeptide. The effects of hydrolysis temperature, pH, enzyme dosage and hydrolysis time on the degree of hydrolysis (DH) were studied. Based on the single factor experiment, Box-Benhnken center composite experiment was carried out with three factors and three levels, including hydrolysis temperature, pH and enzyme dosage. RSM was used to determine the effect of prime factors on the DH and establish a regression model. The results showed that the optimal hydrolysis conditions were as follows: enzyme dosage of 5753 U g −1 dry matter, hydrolysis temperature of 52°C, pH of 8.3, substrate concentration of 5% and hydrolysis time of 5 h. Under the optimal conditions, the DH was up to 16.01%, which matched well with the predicted value. With VC as the positive control, the scavenging ability of the hydrolyzate on DPPH radicals, hydroxyl radicals and superoxide radicals were very powerful, which mean that the hydrolyzate of sea cucumber powder has good antioxidant activity.

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“…In this way, three precipitated fractions were obtained and designated as SPC30, SPC60, and SPC70, respectively. These fractions were deproteinised three times using the Sevag method (Liu et al 2012). The polysaccharide solution and the Sevag reagent (chloroform/n-butanol 4 : 1, v/v) were mixed (polysaccharide solution/Sevag reagent 5 : 1, v/v) and shaken acutely for 30 min, then centrifuged for removing the denaturalised proteins, and the process was repeated 5 times.…”

Section: Methodsmentioning

confidence: 99%

Extraction, fractionation, and chemical characterisation of fucoidans from the brown seaweed Sargassum pallidum

Liu¹,

Liu²,

Wei³

et al. 2016

Czech J. Food Sci.

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Liu X., Liu B., Wie X.L., Sun Z.L., Wang C.Y. (2016): Extraction, fractionation, and chemical characterisation of fucoidans from the brown seaweed Sargassum pallidum. Czech J. Food Sci., 34: 406-413.The fucoidans were obtained by extraction with water and gradient precipitation with different concentrations of ethanol. The main structural characterisations and bioactivities were achieved. Four fractions of water-soluble crude fucoidans with different molecular weights -SPC60, SPC70, SPH60, SPH70 -were extracted from S. pallidum collected from the Yellow Sea, China, using cold water and hot water extraction, and fractional precipitation with gradient concentrations of ethanol. Chemical analysis demonstrated that all of these fucoidan fractions consisted of fucose, rhamnose, xylose, mannose, glucose, and galactose with different monosaccharide mole ratios. Fractions SPC60, SPC70, and SPH70 showed moderate cytotoxic activity against P388 murine leukaemia cells. Fucoidan is a kind of bioactive polysaccharide from the brown algae. This study provides a fast and feasible method to obtain fucoidans from S. pallidum, which can be used as pharmaceutical material and functional food.

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Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus

Cited by 172 publications

References 25 publications

Statistical Data Analysis Which Result From the Bio-Diagnosis and Bio-Treatment of Injured Rats With the Hyperlipidemia and Hyperglycemia Diseases

Statistical Data Analysis Which Result From the Bio-Diagnosis and Bio-Treatment of Injured Rats With the Hyperlipidemia and Hyperglycemia Diseases

Optimization of Enzymatic Hydrolysis of Freeze-Dried Sea Cucumber Powder with Response Surface Methodology and Its Antioxidant Evaluation

Extraction, fractionation, and chemical characterisation of fucoidans from the brown seaweed Sargassum pallidum

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