antioxidant and antihypertensive potential of Ledebouria hyderabadensis

Abstract

Ledebouria hyderabadensis is a new species found in the city limits of Hyderabad, Telangana, India, in 2012. Ethanolic extract of L. hyderabadensis bulbs was measured for its antioxidant and antihypertensive. Phytochemical investigation revealed that the plant contains glycosides, saponins, flavonoids, tannins, carbohydrates, amino acids. Acute toxicity studies performed according to OECD 423 guidelines, where a single dose of 5, 50, 300 and 1000 mg/kg of ethanolic extract of L. hyderabadensis bulbs administered to rats did not displayed any toxic symptoms. Therefore 1000 mg/kg is chosen as the therapeutic dose. The ethanolic extract of L. hyderabadensis bulbs exhibited the DPPH and NO free radical scavenging activity in dose dependent manner than ascorbic acid with IC50 value 54.08 µg/ml and 64.67 µg/ml. The L. hyderabadensis extract exhibited statistically significant (p < 0.05) ACE enzyme inhibition IC50 values (59.64µg/ml) when compared to the standard Captopril (IC50=24.67µg/ml). DOCA salt treatment at the 100 mg/kg, and 200 mg/kg doses, significantly reduced the Mean Systolic Blood Pressure (MSBP) (198.8&169.4) compared with the standard Nifedipine (132.6) and the negative control (122.6).