Antioxidant and antiinflammatory activities of Sideritis perfoliata subsp. perfoliata (Lamiaceae)

Abstract

Sideritis perfoliata L. subsp. perfoliata is a plant widely used in folk medicine in Greece since antiquity because of its antiinflammatory, antirheumatic, antiulcer, digestive and vasoprotective properties. Phytochemical investigations of the polar extracts afforded four flavonoid glycosides, four phenylpropanoic glycosides, caffeic acid and one iridoid, ajugoside. Reactive oxygen species (ROS) are implicated in the aetiology of several inflammatory processes. In the present study polar fractions and isolated compounds from S. perfoliata subsp. perfoliata were evaluated for their antioxidant activity using DPPH spectrophotometric and TBA lipid peroxidation assays, as well as for their antiinflammatory activity using the soybean lipoxygenase bioassay. All extracts and isolated compounds showed significant antioxidant and inhibitory activity against soybean lipoxygenase. These findings give support to the ethnopharmacological use of the plant in the treatment of several inflammatory ailments.

Antioxidant activity of Spathodea campanulata (Bignoneaceae) extracts

“…Inhibition was dose-dependent and it is possible that the identified phenolic compounds (Figure 4) were responsible for this activity. Although the chain break reaction has been postulated as the main mechanism by which phenolic compounds and plant extracts inhibit lipid peroxidation (Charami et al, 2008), the results obtained in this study indicate another major mechanism involved in this process, the ascorbic acid -Fe 3+ mechanism, which induces biological membrane lipid peroxidation. were previously incubated with flower and bark extracts, after which the assay was performed.…”

“…Flav onoids have been shown to inhibit lipid peroxidation and to protect tissue from the damage caused by oxygen reactive species (Laughton et al, 1989;Afanas'Ev et al, 1989), and phenolic compounds usually work as inhibitors of the lipid peroxidation chain reaction (Charami et al, 2008). A large number of studies have been conducted in order to evaluate the antioxidant capacity of phenolic compounds (Kook et al, 2008;Koleckar et al, 2008), though few discuss possible mechanisms of their antioxidant activity.…”

Assessment of effect of hydroalcoholic and decoction methods on extraction of antioxidants from selected Indian medicinal plants

“…Superoxide anion radicals are generated by oxidation of NADH and assayed by the reduction of NBT. The reaction mixture (3.0 mL) consisted of 0.5 mL Tris-HCl buffer (16 mM, pH 8), 0.5 mL NBT (0.3 mM), 0.5 mL NADH (0.936 mM), 0.5 mL PMS (0.12 mM) and 1.0 mL of different concentrations of different solvent extracts. The superoxide radical generating reaction was started by the addition of PMS solution to the mixture.…”

“…Many medicinal plants contain large amount of antioxidants such as polyphenols, which can play an important role in absorbing and neutralizing free radicals, quenching singlet and triplet oxygen or decomposing peroxides [16] . Phenolic compounds in plants provide an array of natural source of antioxidants for use in foods and nutraceutical [17] .…”

Naturally Occurring Iridoids, Secoiridoids and Their Bioactivity. An Updated Review, Part 3

“…perfoliata of Greek origin exhibited weak antioxidative effect on DPPH radical in the range of 6.3-23.6% at a concentration of 0.1 mM in spectrophotometric assay method. 81) h. Anti-protozoal Activity Iridoids, epoxygaertneroside (188), methoxygaertneroside (189), gaertneroside (190), acetylgaertneroside (191) and gaertneric acid (192) isolated from 80% methanolic extract of Morinda morindoides leaves showed significant antiamoebic activity against Entamoeba histolytica with IC 50 values of 1.3, 2.3, 4.3, 5.4 and 7.1 mg/ ml, respectively. All these iridoids were devoid of any cytotoxic effect against MT-4 cells at the highest test concentration of 250 mg/ml.…”