Antioxidant and Antiinflammatory Activities of Anthocyanins and Their Aglycon, Cyanidin, from Tart Cherries

Abstract

The anthocyanins (1-3) and cyanidin isolated from tart cherries exhibited in vitro antioxidant and antiinflammatory activities comparable to commercial products. The inhibition of lipid peroxidation of anthocyanins 1-3 and their aglycon, cyanidin, were 39, 70, 75, and 57%, respectively, at 2-mM concentrations. The antioxidant activities of 1-3 and cyanidin were comparable to the antioxidant activities of tert-butylhydroquinone and butylated hydroxytoluene and superior to vitamin E at 2-mM concentrations. In the antiinflammatory assay, cyanidin gave IC50 values of 90 and 60 mM, respectively, for prostaglandin H endoperoxide synthase-1 and prostaglandin H endoperoxide synthase-2 enzymes.

Efficacy of Tart Cherry Juice to Reduce Inflammation Biomarkers among Women with Inflammatory Osteoarthritis (OA)

“…Cyclo-oxygenase inhibitory flavonoids (Seeram et al, 2001;Wang et al, 1999) and anthocynanins with high antioxidant and anti-inflammatory activities (Blando et al, 2004;Tall et al, 2004) have been identified in tart cherries, which are considered good sources of phenolic compounds. This has led to speculation that tart cherry consumption may be effective in alleviating symptoms in inflammatory conditions (Tall et al, 2004).…”

“…Recently, numerous antioxidant and anti-inflammatory agents have been identified in plants purported to reduce illness and disease associated with inflammation and tissue damage. Specifically, the disease modifying agents include cyclo-oxygenase inhibitory flavonoides (Seeram, Bourquin, & Nair, 2001;Wang et al, 1999) and anthocynanins with high antioxidant and anti-inflammatory activities (Blando, Gerardi, & Nicoletti, 2004;Tall et al, 2004). These have been identified in natural foods from black tea to tart cherries to fish oil.…”

Phytochemical uptake following human consumption of Montmorency tart cherry (L. Prunus cerasus) and influence of phenolic acids on vascular smooth muscle cells in vitro

“…Despite previous studies in cell culture and animal 40 models, where cherry extracts have been shown to exert a range of cardio-protective effects [12,5], there has…”

Inhibitory Effects of Phenylpropanoids Isolated from the Bark of Ailanthus altissima on COX-2 Activity

“…5 mg protein/mL in 0.1 M Tris/HCl, pH 7.8), a homogeneous protein purified from ram seminal according to the previously published procedures. 26 The rate of oxygen consumption during the initial phase of the enzyme-mediated reaction, with arachidonic acid as substrate was measured using a Model 5300 biological oxygen monitor (Yellow Spring Instruments, Inc., Yellow Springs, OH). Each assay mixture consisted of 0.1 M Tris/HCl, pH 7.8, 1.0 mM phenol, 17 µg hemoglobin, and 10 µM arachidonic acid.…”