Antioxidant and Antimicrobial Activities of the Extracts from Different Garcinia Species

Abstract

Background. Garcinia is a large genus which has promising bioactivities. However, the properties of many Garcinia species have not been investigated thoroughly. Aim. To determine the antioxidant and antimicrobial capabilities of the extracts from different Garcinia species. Methodology. Six Garcinia species, including Garcinia fusca, Garcinia hopii, Garcinia planchonii, Garcinia nigrolineata, Garcinia gaudichaudii, and Garcinia tinctoria were extracted using n-hexane, ethyl acetate, and methanol, producing n-hexane extract (HE), ethyl acetate extract (EAE), and methanol extract (ME). After that, the total polyphenol content was evaluated using Folin–Ciocalteu assay. DPPH, hydroxyl radical scavenging, and total antioxidant capacity assays were performed to test the antioxidant activity. Subsequently, the antimicrobial activities against Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacterial strains were assessed using Kirby Bauer and the broth microdilution methods. Results. Many Garcinia extracts contained high total polyphenol content consisting of ME of G. hopii ad G. tinctoria, and EAE of G. planchonii and G. tinctoria. The EAE of G. tinctoria showed effective antioxidant capacity (IC50 = 1.5 µg/mL). Additionally, the EAE of G. gaudichaudii was effective against Gram-positive bacteria with minimal inhibition concentration (MIC) of 15.625–25 µg/mL whereas ME of G. planchonii was effective against both Gram-positive bacteria (MIC = 160 µg/mL) and Gram-negative bacteria (MIC = 75 µg/mL). Conclusion. Several extracts of Garcinia species demonstrated valuable antioxidant and antimicrobial properties.

Evaluation of Antibacterial and Antibiofilm Activity of Rice Husk Extract against Staphylococcus aureus

“…Subsequently, a drop (10 µL) of each concentration (20×, 40×, 60×, 70× and 80×) of the husk extract was placed on the surface of the BHI agar plates; 10% ethanol (RHE resuspension medium) was used as a negative control. After incubation for 24 h at 37 • C in aerobic conditions, the halo zone of inhibition was measured [14].…”

Antioxidant property of secondary metabolites from Garcinia genus: A short review

“…The parts of the same Garcinia species were compared in terms of antioxidant activity and total phenolic content and total flavonoids content. Nguyen et al (2021) evaluated the antioxidant activity of G. fusca, G. hopii, G. planchonii, G. nigrolineata, G. gaudichaudii, and G. tinctoria. The extracts were obtained with hexane, ethyl acetate and methanol in solid-liquid extraction.…”