Antioxidant and antimutagenic activities of pomegranate peel extracts

Negi, Pradeep Singh; Jayaprakasha, G.K.; Jena, B.S.

doi:10.1016/s0308-8146(02)00279-0

Cited by 455 publications

(306 citation statements)

References 26 publications

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“…The antimutagenic effect was considered negligible when a value lower than 25% was obtained, moderate when a value between 25% and 40% was obtained and strong at values greater than 40% (Neigi et al, 2003).…”

Section: Ames Antimutagenicity Assaymentioning

confidence: 99%

Assessment of estrogenic, mutagenic and antimutagenic activity of nemorosone

Camargo¹,

Varela²,

Oliveira³

et al. 2011

Rev. bras. farmacogn.

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Currently, a wide range of research involving natural products is focused on the discovery of new drugs in many different therapeutic areas. A great number of the synthetic compounds on the market were derived from natural products, especially plants. Nemorosone is the major constituent of the floral resin of Clusia rosea Jacq., Clusiaceae, and in Cuban propolis. In vitro studies have shown cytotoxic activity in this substance against various tumor cell lines, including those resistant to various cytotoxic drugs, whereas it has low cytotoxicity to non-tumoral cells. Therefore, in order to characterize the biological activity of nemorosone, a substance with potential antitumor activity, and in view of preclinical testing of the toxicity of drug candidate compounds, the main aim of this study was to determine the mutagenic and antimutagenic activity of nemorosone by the Ames test, using the strains TA97a, TA98, TA100 and TA102 of Salmonella typhimurium. Secondly, to characterize the estrogenic activity in an experimental recombinant yeast model (Recombinant Yeast Assay) mutagenic activity was observed at in any of the concentrations in any of the test strains. To evaluate the antimutagenic potential, direct and indirect mutagenic agents were used: 4 nitro-o-phenylenediamine (NPD), mitomycin C (MMC) and aflatoxin B1 (AFL). Nemorosone showed moderate antimutagenic activity (inhibition level 31%), in strain TA100 in the presence of AFL, and strong antimutagenic activity in TA102 against MMC (inhibition level 53%). Estrogenic activity was observed, with an EEq of 0.41±0.16 nM at various tested concentrations.

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Section: Ames Antimutagenicity Assaymentioning

confidence: 99%

Assessment of estrogenic, mutagenic and antimutagenic activity of nemorosone

Camargo¹,

Varela²,

Oliveira³

et al. 2011

Rev. bras. farmacogn.

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“…The antioxidant activities of P. granatum are associated with different bioactive components, mainly polyphenols, ellagitannins, condensed tannins, and anthocyanins (Kulkarni et al, 2004;Li et al, 2006;Negi et al, 2003;Noda et al, 2002). In this regard, West et al (2007) showed that polyphenols present in this plant protect neonatal mouse brain against hypoxic-ischemic injury.…”

Section: Discussionmentioning

confidence: 98%

“…Recently, the antioxidant activity of P. granatum associated with its phytochemicals, such as, polyphenols, flavonoids, and anthocyanidins has gained importance (Faria et al, 2007;Guo et al, 2007;Kulkarni et al, 2007;Mirdehghan et al, 2007;Reddy et al, 2007;Rout, Banerjee, 2007;Sestili et al, 2007;Zaid et al, 2007). In this regard, Negi et al (2003) demonstrated that the in vitro antioxidant ability of the pomegranate fruit, rich in polyphenols and anthocyanidins, was higher than that found in green tea, also considered a powerful antioxidant. Several extracts/constituents of P. granatum have been found to prevent low-density lipoprotein oxidation and hence are antiarthrogenic (Aviram et al, 2002;Wang et al, 2004), modulating the expression of oxidation-sensitive genes in vitro and in hypercholesterolemic mice (De Nigris et al, 2005), as well as inhibiting the nuclear factor κβ, which is activated by reactive oxygen species (ROS) (Afaq et al, 2005).…”

mentioning

confidence: 99%

Assessment of mutagenic and antimutagenic effects of Punica granatum in mice

Valadares

Pereira

Benfica

et al. 2010

Braz. J. Pharm. Sci.

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In the present study, the ability of Punica granatum ethanolic leaf extract (PGL) and Punica granatum ethanolic fruit extract (PGF) to induce mutagenicity or to modulate the genotoxic effects induced by the alkylating agent cyclophosphamide (CP) was evaluated. Swiss male mice were treated by gavage for 10 days with PGL or PGF (12.5, 25, 50, and 75 mg/kg/day) prior to exposure to CP (i.p. 200 mg/kg), 24 h after the end of the treatment. Initial observations revealed that normal mice treated with both extracts (12.5, 25, 50, and 75 mg/kg/day) showed a similar micronucleated polychromatic erythrocyte (MNPCE) frequency to that of the control group. Investigation of the protective effect of PGL and PGF based on data analysis revealed that, irrespective of dose or extract, oral administration of PGL or PGF for 10 days prior to exposure had reduced, in a dose-dependent manner, the frequency of MNPCE induced by CP in all groups studied. Higher reductions were observed at PGF doses of 50 and 75 mg/kg. Taken together, these results demonstrate that mice treated with P. granatum showed an absence of mutagenic effects and dose-dependent protective effects against CP-induced oxidative DNA damage. Uniterms:Punica granatum/mutagenic effects. Punica granatum/antimutagenic effects. Micronucleic. Genotoxicity. Cyclophosphamide.No presente estudo investigamos o potencial do extrato etanólico das folhas da Punica granatum (PGFO) e do extrato etanólico dos frutos da Punica granatum (PGFR) de induzir mutagenicidade ou de proteger contra efeitos genotóxicos induzidos pela ciclofosfamida (CF). Camundongos machos Swiss foram tratados por 10 dias, via oral, com PGFO ou PGFR (12,5, 25, 50 e 75 mg/kg/dia), previamente a exposição à CF (i.p. 200 mg/kg) 24 horas após término do tratamento. Observamos que os animais tratados por 10 dias com ambos os extratos (12,5, 25, 50 e 75 mg/kg/dia) demonstraram a frequência de micronúcleo policromático eritrocitário (MNPCE) similar ao grupo controle. Quando aos efeitos protetores dos extratos foram investigados, a análise dos dados revelou que, independentemente da dose ou do extrato usado, a administração oral por 10 dias, previamente à exposição, reduziu, de forma dose-dependente, a frequência de MNPCE induzidos pela CF, em todos os grupos estudados. As maiores reduções foram observadas com PGFR nas doses de 50 e 75 mg/kg. Em conjunto, sob as condições testadas, camundongos tratados com P. granatum demonstraram ausência de efeitos mutagênicos e, de forma dose-dependente, efeitos protetores contra os danos oxidativos do DNA induzidos pela CF. Unitermos:Punica granatum/efeitos mutagênicos. Punica granatum/efeitos antimutagênicos. Micronúcleo. Genotoxicidade. Ciclofosfamida. INTRODUCTIONPunica granatum Linn., a Punicaceae, commonly known as pomegranate, has a vast ethnomedical history and represents a phytochemical reservoir of heuristic medicinal value (Lansky and Newman, 2007). The plant as a whole and especially its flowers, have been extensively used in the ancient Ayurvedic health care system (Siv...

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“…They have been used traditionally for their medicinal properties as anticancer, anti-inflammatory, antioxidant and antithelminthic [37,38] and for other purposes such as tanning, dyeing [39,40] and heavy metal removal [41]. Pomegranate peels are characterized by an interior network of membranes comprising almost 26-30% of total fruit weight and are characterized by substantial amounts of phenolic compounds, including flavonoids (anthocyanins, catechins and other complex flavonoids) and hydrolysable tannins (punicalin, pedunculagin, punicalagin, gallic and ellagic acid) [42][43][44]. Gallic acid, ellagic acid and punicalagin, in addition to their free radical-scavenging properties, also possess antibacterial activites against intestinal flora, particularly enteric pathogens, i.e., Escherichia coli, Salmonella spp.…”

Section: Pomegranatementioning

confidence: 99%