Antioxidant and antimutagenic potential of Psidium guajava leaf extracts

Abstract

Fruits, vegetables and medicinal herbs rich in phenolics antioxidants contribute toward reduced risk of age-related diseases and cancer. In this study, Psidium guajava leaf extract was fractionated in various organic solvents viz. petroleum ether, benzene, ethyl acetate, ethanl and methanol and tested for their antioxidant and antimutagenic properties. Methanolic fraction showed maximum antioxidant activity comparable to ascorbic acid and butylated hydroxyl toluene (BHT) as tested by DPPH free radical scavenging, phosphomolybdenum, FRAP (Fe3 + reducing power) and CUPRAC (cupric ions (Cu) reducing ability) assays. The fraction was analyzed for antimutagenic activities against sodium azide (NaN), methylmethane sulfonate (MMS), 2-aminofluorene (2AF) and benzo(a)pyrene (BP) in Ames Salmonella tester strains. The methanol extracted fraction at 80 μg/ml concentration inhibited above 70% mutagenicity. Further, phytochemical analysis of methanol fraction that was found to be most active revealed the presence of nine major compounds by gas chromatography-mass spectrometry (GC-MS). This data suggests that guava contains high amount of phenolics responsible for broad-spectrum antimutagenic and antioxidant properties in vitro and could be potential candidates to be explored as modern phytomedicine.

Leaf Extracts of Mangifera indica L. Inhibit Quorum Sensing – Regulated Production of Virulence Factors and Biofilm in Test Bacteria

“…The mature dried leaves were finely ground and extract was prepared as described previously (Zahin et al, 2013, 2016). The extracts and fractions were dried by rotatory evaporator at 40°C followed by successive extraction of collected dried plant material with other solvents (benzene, ethyl acetate, acetone, methanol and ethanol).…”

“…The compositions of the ML extract was analyzed by gas chromatography as described earlier by Zahin et al (2016).…”

Phytochemical Content, Antidiabetic, Anticholinergic, and Antioxidant Activities of Endemic Lecokia cretica Extracts

“…For example, using the DPPH method, it was reported that petroleum ether and methanol fractions of P. guajava leaf showed approximately 5 % and 34 % decolorization, respectively. This could be due to the polarity of the solvents, which enable to extract a large number of compounds …”

Protective effect of juice extract of mesocarp rind of Citrullus lanatus in carbon tetrachloride-induced oxidative stress in rats

“…The juice or pulp of C. lanatus is consumed by people, while the rind and the seeds are usually discarded as agro-wastes [20][21][22]. Pectin can be extracted from the rind and the rind can also be used for products like pickles and preserves [23,24]. The rind is edible and sometimes used as vegetable.…”