Antioxidant and chemoprotective potential of Streptomyces levis strain isolated from human gut

Abstract

In the current study, Streptomyceslevis strain HFM-2 has been isolated from healthy human gut. Streptomyces sp. HFM-2 was identified based on the polyphasic approach that included cultural, morphological, chemotaxonomical, phylogenetic, physiological, and biochemical characteristics. 16S rRNA gene sequence of strain HFM-2 exhibited 100% similarity with Streptomyceslevis strain 15423 (T). The EtOAc extract of Streptomyceslevis strain HFM-2 showed potential antioxidant activity, along with 69.53 ± 0.19%, 64.76 ± 0.13%, and 84.82 ± 0.21% of scavenging activity for ABTS, DPPH, and superoxide radicals, respectively at 600 µg/mL. The IC50 values i.e. 50% scavenging activity for DPPH, ABTS, and superoxide radicals were achieved at 497.19, 388.13, and 268.79 (µg/mL), respectively. The extract's reducing power and total antioxidant capacity were determined to be 856.83 ± 0.76 and 860.06 ± 0.01 µg AAE/mg of dry extract, respectively. In addition, the EtOAc extract showed protection against DNA damage from oxidative stress caused by Fenton’s reagent, and cytotoxic activity against HeLa cervical cancer, Skin (431) cancer, Ehrlich-Lettre Ascites-E (EAC) carcinoma, and L929 normal cell lines. The IC50 values against HeLa, 431 skin, and EAC carcinoma cell lines were found to be 50.69, 84.07, and 164.91 µg/mL, respectively. The EtOAc extract showed no toxicity towards L929 normal cells. In addition, flow cytometric analysis exhibited reduced mitochondrial membrane potential (MMP), and enhanced levels of reactive oxygen species (ROS). The EtOAc extract was chemically analyzed using GCMS to determine the components executing its bioactivities.

Probiotic potential of Streptomyces levis strain HFM-2 isolated from human gut and its antibiofilm properties against pathogenic bacteria

“…Streptomyces isolate HFM-2 used in the present study was isolated from healthy human gut [ 22 ]. For the isolation of Streptomyces , stool samples were collected from healthy human hosts after taking their informed consent from the laboratory of Dr. Sukhraj Kaur, Department of Microbiology, G.N.D.U., Amritsar.…”

“…The antibacterial metabolite production by Streptomyceslevis strain HFM-2 was carried out as described by Verma et al [ 22 ]. The fermentation process was carried out in Erlenmeyer flasks on a rotary shaker at 180 rpm after the production medium was inoculated with Streptomyces culture.…”

Streptomyces at the Heart of Several Sectors to Support Practical and Sustainable Applications: A Review

“…QZS11 Soil Ethyl-acetate crude extract [210] Streptomyces sp.MA4 Soil Selenium nanoparticles [211] Streptomyces sp. Sae4034 Rhizosphere soil Alkaloid, terpenoid, flavonoid, and polyphenol [212] Streptomyces levis HFM-2 Human gut 2-Isopropyl-5-methyl-1-heptanol; 1-Octanol, 2-butyl-, 3-Octadecene and 3-Eicosene [213] Streptomyces sp. MUSC Streptomyces colonosanans MUSC 93J T Mangrove soil Ectoine synthesis gene [223] Streptomyces monashensis MUSC 1J T Mangrove soil phenolic compounds, pyrazines and pyrrolopyrazines, Benzoic acid, 9H-Pyrido[3,4-b]indole [224] 6.…”