Antioxidant and Cytoprotective Effect of Piper aduncum L. against Sodium Fluoride (NaF)-Induced Toxicity in Albino Mice

Abstract

Piper aduncum, commonly known as matico, is a plant that grows in the mountainous and coastal regions of Peru, and is studied for its antimicrobial properties and various ethnopharmacological uses. The main objective of this study was to determine the cytoprotective and antioxidant effects of the methanolic extract of Piper aduncum leaves in Mus musculus previously administered with sodium fluoride (NaF) using the Micronucleus test and the Comet assay. The extract was administrated orally in four different concentrations: 150, 300, 600, and 1200 mg/Kg for ten days. At the 11th day, a single dose of NaF was administrated via intraperitoneal at 20 mg/Kg. The genotoxicity study was performed with mice from the strain BALB/c, using the Micronucleus test on bone marrow and the Comet assay on peripheral blood according to OECD guidelines 474 and 489, respectively. The statistical analysis was performed by median analysis with ANOVA. Significant differences were found in Micronucleus frequency between the highest concentrations of Piper aduncum and NaF. The Comet assay showed significant reduction of NaF-induced damage on erythrocytes depending on the different concentrations of the extract which were evaluated in this study. It is concluded that the methanolic extract of P. aduncum leaves has cytoprotective and antioxidant activity against sodium fluoride.

Brief Review on Piper aduncum L., its Bioactive Metabolites and its Potential to Develop Bioproducts

“…Recent findings have reinforced that P. aduncum extracts have exhibited antileishmanial, antibacterial, cytotoxic and antifungal activities [24]. Furthermore, compounds isolated from its leaves have shown to be very active against promastigote and intracellular amastigotes (which cause damage to DNA), mainly due to their antileishmanial, antimicrobial, molluscoidal, antitumor and antifungal activities [24].…”

Ameliorative Effect of the Oral Administration of Chuquiraga spinosa in a Murine Model of Breast Cancer Induced with 7,12-Dimethylbenz[a]anthracene (DMBA)

“…9 It is used as diuretic, anti-inflammatory, renal debugger and prostate cancer. 10 The present study aimed to determine the antitumor, antioxidant and antigenotoxic effect of the ethanolic extract of the aerial parts of ChS on DMBA-induced breast cancer in a murine model.…”

Influencia de las Condiciones de Extracción sobre los Compuestos Polifenólicos Totales del Piper Aduncum L

“…3885 de extracción 30 min. La investigación desarrollada porHerrera-Calderon et al (2019), reportó resultados inferiores utilizando como solvente metanol, dando valores que van desde 1.22 a 1.61 g equivalentes de ácido gálico/ 100 g ps. El solvente utilizado en la extracción juega un papel importante en el rendimiento de los compuestos bioactivos ya que su concentración puede variar; en este sentido, Santillán y Quintana (2020) en su investigación menciona que el agua, en comparación con etanol y metanol, es el mejor solvente para aumentar los rendimientos de extracción de los CPT.…”