Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Aboushanab, Saied A.; Шевырин, Вадим А.; Slesarev, Grigory P.; Melekhin, Vsevolod V.; Shcheglova, Anna V.; Makeyev, Oleg H.; Kovaleva, Elena G.; Kim, Ki Hyun

doi:10.3390/plants11060741

Cited by 12 publications

(11 citation statements)

References 73 publications

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“…The lowest TFC and TPC were achieved at 60°C by dynamic maceration with EtOH-H 2 O 7:3 (v/v), 136.30 ± 23.8 μg QE/g, and gas-expanded ChCl:Ca:H 2 O, which content was not detected, respectively. Aboushanab et al extracted isoflavones from kudzu roots and soy molasses using choline chloride and citric acid at a 1:2 molar ratio ( 68 ). They achieved the TPC and TFC of 15.20 ± 3.47 mg GAE/g and 6.12 ± 0.51 mg QE/g, respectively, in soy molasses extracts.…”

Section: Resultsmentioning

confidence: 99%

“…The comparison between the quoted results with our work is inadequate since they washed three times their NADES extracts with ethyl acetate 1:3 (v/v) ratio and concentrated such fractions. The dried extracts were used to perform the assays, promoting a higher concentration of TPC and TFC ( 68 ). Combining pressurized hot water extraction (PHWE) and NADES as modifiers, Loarce et al achieved a promising approach to extract anthocyanins from grape pomace ( 22 ).…”

Section: Resultsmentioning

confidence: 99%

“…T ( • C) TFC (µ g QE/g) TPC (mg GAE/g) (68). They achieved the TPC and TFC of 15.20 ± 3.47 mg GAE/g and 6.12 ± 0.51 mg QE/g, respectively, in soy molasses extracts.…”

Section: Extractsmentioning

confidence: 95%

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Pressurized natural deep eutectic solvents: An alternative approach to agro-soy by-products

et al. 2022

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Soybeans are mainly used for food and biodiesel production. It is estimated that soy crops worldwide will leave about 651 million metric tons of branches, leaves, pods, and roots on the ground post-harvesting in 2022/23. These by-products might serve as largely available and cheap source of high added-value metabolites, such as flavonoids, isoflavonoids, and other phenolic compounds. This work aimed to explore green approaches based on the use of pressurized and gas expanded-liquid extraction combined with natural deep eutectic solvents (NADESs) to achieve phenolic-rich extracts from soy by-products. The total phenolic and flavonoid contents of the generated extracts were quantified and compared with conventional solvents and techniques. Pressurized liquid extraction (PLE) with choline chloride/citric acid/water (1:1:11 – molar ratio) at 120°C, 100 bar, and 20 min, resulted in an optimized condition to generate phenolic and flavonoid-rich fractions of soy by-products. The individual parts of soy were extracted under these conditions, with their metabolic profile obtained by UHPLC-ESI-QToF-MS/MS and potential antioxidant properties by ROS scavenging capacity. Extracts of soy roots presented the highest antioxidant capacity (207.48 ± 40.23 mg AA/g), three times higher than soybean extracts (68.96 ± 12.30). Furthermore, Hansen solubility parameters (HSPs) were applied to select natural hydrophobic deep eutectic solvents (NaHDES) as substituents for n-heptane to defat soybeans. Extractions applying NaHDES candidates achieved a similar yield and chromatography profile (GC-QToF-MS) to n-heptane extracts.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Pressurized natural deep eutectic solvents: An alternative approach to agro-soy by-products

et al. 2022

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“…NADESs are composed of natural compounds produced by cell metabolism, and they have similar characteristics to DES. For the extraction of puerarin isoflavones, Saied A [ 81 ] created NADESs with a molar ratio of choline chloride to citric acid of 1:2. A high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS) was used to detect the extract of KR, and 10 isoflavones were detected.…”

Section: Sample Pretreatment Methodsmentioning

confidence: 99%

Advances in Extraction, Purification, and Analysis Techniques of the Main Components of Kudzu Root: A Comprehensive Review

Xuan,

Liu,

Liu

et al. 2023

Molecules

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Kudzu root (Pueraria lobate (Willd.) Ohwi, KR) is an edible plant with rich nutritional and medicinal values. Over the past few decades, an ample variety of biological effects of Pueraria isoflavone have been evaluated. Evidence has shown that Pueraria isoflavone can play an active role in antioxidant, anti-inflammatory, anti-cancer, neuroprotection, and cardiovascular protection. Over 50 isoflavones in kudzu root have been identified, including puerarin, daidzein, daidzin, 3′-hydroxy puerarin, and genistein, each with unambiguous structures. However, the application of these isoflavones in the development of functional food and health food still depends on the extraction, purification and identification technology of Pueraria isoflavone. In recent years, many green and novel extraction, purification, and identification techniques have been developed for the preparation of Pueraria isoflavone. This review provides an updated overview of these techniques, specifically for isoflavones in KR since 2018, and also discusses and compares the advantages and disadvantages of these techniques in depth. The intention is to provide a research basis for the green and efficient extraction, purification, and identification of Pueraria isoflavone and offers investigators a valuable reference for future studies on the KR.

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“…New cancer survival and mortality control techniques are needed ( Da Silva et al, 2016 ; Dai et al, 2017 ; Pucci et al, 2019 ; Mahdinloo et al, 2020 ; Bagherifar et al, 2021 ). As an alternative or complementary to traditional chemo or radiotherapy, naturally occurring phytochemicals (such as flavonoids, isoflavones, and lignans) with antioxidant or hormone-like actions are being explored for cancer ( Ghosh et al, 2021a ; Ahmad et al, 2021 ; Aboushanab et al, 2022 ; Lim & Park, 2022 ). These cancer-suppressing phytochemicals act mainly by affecting the signaling molecules that have either been abnormally activated or silenced.…”

Section: Introductionmentioning

confidence: 99%

Use of gold nanoparticle-silibinin conjugates: A novel approach against lung cancer cells

Ravi

Zeyaullah²,

Ghosh³

et al. 2022

Front. Chem.

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Lung cancer presents one of the most challenging carcinomas with meager 5-year survival rates (less than 20%), high metastasis and high recurrence due to chemo- and radio- resistance. An alternative or complementation to existing prognosis modalities is the use of phytochemicals such as silibinin, which targets essential cytokines, angiogenic factors and transcription factors for a profound anti-tumor effect. However, the problems of low solubility in an aqueous physiological environment, poor penetration, high metabolism and rapid systemic clearance limit the therapeutic use of silibinin. Conjugation of gold nanoparticles (GNPs) with silibinin may overcome the above challenges along with distinct advantages of biocompatibility, optical properties for monitoring and causation of cytotoxicity in cancer cells. The current study thus aims to develop silibinin conjugated gold nanoparticles (Sb-GNPs) with pH responsive release in the cancer microenvironment, optimizing several parameters for its higher activity and further evaluate the nanoplatform for their efficacy in inducing cell death in vitro against A549 lung cancer cells. GNPs was synthesized using trisodium citrate dihydrate as the reducing agent and further used for the conjugation of silibinin. The synthesized GNPs were found to be monodispersed and spherical in shape. The silibinin was successfully conjugated with gold nanoparticles and long-term stability of GNPs and Sb-GNPs nanoconjugates in suspension phase was confirmed by FTIR and DLS. Anticancer properties of Sb-GNPs were confirmed by different assay using MTT, Trypan blue dye exclusion assay and cell cycle analysis assay. After conjugation of silibinin with GNPs, the efficacy of silibinin increased 4–5 times in killing the cancer cells. This is the first report on using silibinin gold nanoconjugate system for lung cancer therapy with promising future applications.

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Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Cited by 12 publications

References 73 publications

Pressurized natural deep eutectic solvents: An alternative approach to agro-soy by-products

Pressurized natural deep eutectic solvents: An alternative approach to agro-soy by-products

Advances in Extraction, Purification, and Analysis Techniques of the Main Components of Kudzu Root: A Comprehensive Review

Use of gold nanoparticle-silibinin conjugates: A novel approach against lung cancer cells

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