Antioxidant and Cytotoxic Activities of Exopolysaccharides from Alcaligenes faecalis Species Isolated from the Marine Environment of Mauritius

Aullybux, Aadil Ahmad; Puchooa, Daneshwar; Bahorun, Theeshan; Jeewon, Rajesh; Wen, Xuesong; Matin, Poona

doi:10.1007/s10924-021-02290-4

Cited by 9 publications

(4 citation statements)

References 95 publications

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“…The molecular weight of EPS RT7 was obtained via gel filtration chromatography with a Sephadex G-100 column (1.6 × 50 cm) eluting with 0.2 mol −1 NaCl solution at a flow rate of 1 mL/min. Standard reference dextrans (5–80 KDa) were used [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Production and Characterisation of an Exopolysaccharide by Bacillus amyloliquefaciens: Biotechnological Applications

et al. 2023

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The Bacillus amyloliquefaciens RT7 strain was isolated from an extreme acidic environment and identified. The biodegradation capabilities of the strain using different carbon sources (glucose, oleic acid, Tween 80, PEG 200, and the combination of glucose–Tween 80) were evaluated via an indirect impedance technique. The glucose–Tween 80 combination was further studied using nuclear magnetic resonance (NMR). The exopolysaccharide (EPSRT7) that had been produced with the strain when biodegrading glucose–Tween 80 was isolated and characterised using different techniques (GC–MS, HPLC/MSMS, ATR–FTIR, TGA, and DSC), and its molecular weight was estimated. The results show that the average molecular weight of EPSRT7 was approximately 7.0794 × 104 Da and a heteropolysaccharide composed of mannose, glucose, galactose, and xylose (molar ratio, 1:0.5:0.1:0.1) with good thermostability. EPSRT7 showed good emulsifying activity against different natural oils and hydrocarbons at high concentrations (2 mg/mL) and at the studied pH range (3.1–7.2). It also presented good emulsifying activity compared to that of commercial emulsifiers. Lastly, EPSRT7 showed antioxidant capacity for different free radicals, a lack of cytotoxicity, and antioxidant activity at the cellular level. EPSRT7 has promising applications in bioremediation processes and other industrial applications.

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Section: Methodsmentioning

confidence: 99%

Production and Characterisation of an Exopolysaccharide by Bacillus amyloliquefaciens: Biotechnological Applications

et al. 2023

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“…The marine microorganisms have unique metabolic and physiological abilities, which give them the ability to produce various biological compounds [ 122 , 123 , 124 ], such as EPS. Several marine microbial EPS have been reported to have anticancer activity by the dysfunction of mitochondria, the inhibition of cell proliferation, or the modulation of the immune system [ 42 , 58 , 94 , 125 , 126 , 127 , 128 ]. Matsuda et al studied the marine Pseudomonas polysaccharide B1, and found that it could induce U937 cells’ apoptosis [ 128 ].…”

Section: The Biological Activities Of Marine Epsmentioning

confidence: 99%

Exopolysaccharides from Marine Microbes: Source, Structure and Application

Zheng

et al. 2022

Marine Drugs

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The unique living environment of marine microorganisms endows them with the potential to produce novel chemical compounds with various biological activities. Among them, the exopolysaccharides produced by marine microbes are an important factor for them to survive in these extreme environments. Up to now, exopolysaccharides from marine microbes, especially from extremophiles, have attracted more and more attention due to their structural complexity, biodegradability, biological activities, and biocompatibility. With the development of culture and separation methods, an increasing number of novel exopolysaccharides are being found and investigated. Here, the source, structure and biological activities of exopolysaccharides, as well as their potential applications in environmental restoration fields of the last decade are summarized, indicating the commercial potential of these versatile EPS in different areas, such as food, cosmetic, and biomedical industries, and also in environmental remediation.

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“…Molecular weight of the purified fraction was determined by gel filtration chromatography [22]. Standard reference dextrans of 5, 12, 50 and 80 KDa (Sigma, St. Louis, MO, USA) molecular weight were used.…”

Section: Compositional Analysis and Characterisation Of The Eps 251 C...mentioning

confidence: 99%

“…Previous studies showed Bacillus strains with metal chelating properties, such as Bacillus haynesii [15], Bacillus firmus [17] and Bacillus cereus [18]. In addition, numerous studies have been performed to search for biomedical applications [19][20][21][22]. In industrial applications, emulsifiers based on polysaccharides of microbial origin have attracted considerable attention in recent years [23] as an alternative to chemical-based emulsifiers, which are extensively used in various industries and have raised concerns associated with their toxic effects on the environment [24], e.g., Bacillus mojavensis [25] or Bacillus albus [26].…”

Section: Introductionmentioning

confidence: 99%

Potential Applications of an Exopolysaccharide Produced by Bacillus xiamenensis RT6 Isolated from an Acidic Environment

et al. 2022

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The Bacillus xiamenensis RT6 strain was isolated and identified by morphological, biochemical and molecular tests from an extreme acidic environment, Rio Tinto (Huelva). Optimisation tests for exopolysaccharide (EPS) production in different culture media determined that the best medium was a minimal medium with glucose as the only carbon source. The exopolymer (EPSt) produced by the strain was isolated and characterised using different techniques (GC-MS, HPLC/MSMS, ATR-FTIR, TGA, DSC). The molecular weight of EPSt was estimated. The results showed that the average molecular weight of EPSt was approximately 2.71 × 104 Da and was made up of a heteropolysaccharide composed of glucose (60%), mannose (20%) and galactose (20%). The EPSt showed antioxidant capabilities that significantly improved cell viability. Metal chelation determined that EPSt could reduce the concentration of transition metals such as iron at the highest concentrations tested. Finally, the emulsification study showed that EPSt was able to emulsify different natural polysaccharide oils, reaching up to an 80% efficiency (olive and sesame oil), and was a good candidate for the substitution of the most polluting emulsifiers. The EPSt was found to be suitable for pharmaceutical and industrial applications.

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Antioxidant and Cytotoxic Activities of Exopolysaccharides from Alcaligenes faecalis Species Isolated from the Marine Environment of Mauritius

Cited by 9 publications

References 95 publications

Production and Characterisation of an Exopolysaccharide by Bacillus amyloliquefaciens: Biotechnological Applications

Production and Characterisation of an Exopolysaccharide by Bacillus amyloliquefaciens: Biotechnological Applications

Exopolysaccharides from Marine Microbes: Source, Structure and Application

Potential Applications of an Exopolysaccharide Produced by Bacillus xiamenensis RT6 Isolated from an Acidic Environment

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