Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves

Casagrande, Junior Cesar; Macorini, Luís Fernando Benitez; Antunes, Kátia Ávila; Santos, Uilson Pereira dos; Campos, Jaqueline Ferreira; Dias-Júnior, Nelson Miguel; Sangalli, Andréia; Cardoso, Cláudia Andréa Lima; Vieira, Maria do Carmo; Rabelo, Luíza Antas; Paredes‐Gamero, Edgar Julian; Santos, Edson Lucas dos; Souza, Kely de Picoli

doi:10.1371/journal.pone.0112748

Cited by 32 publications

(40 citation statements)

References 48 publications

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“…Phenolic compounds are hydrogen donors capable of directly scavenging free radicals and reducing oxidative damage [42,43], which makes them potent antioxidants. In other medicinal plant extracts, these compounds also activated endogenous antioxidant systems and inhibited the lipid peroxidation of human erythrocytes [2, 44]. Among the phenolic compounds, flavonoids are the most prominent because of their potent antioxidant activity [45].…”

Section: Discussionmentioning

confidence: 99%

“…This biome has a wide variety of medicinal plants used in folk medicine. Many of these plants have been investigated, and their antioxidant [2], antimicrobial [3], antidiabetic [4], anti-inflammatory [5], and cytotoxic [6] activities, among others, have been scientifically proven.…”

Section: Introductionmentioning

confidence: 99%

“…Among these compounds, phenolic compounds and flavonoids from several plant species have cytotoxic activities against different cell lines, including leukemic cell lines [2,25,26]. …”

Section: Introductionmentioning

confidence: 99%

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Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes

et al. 2016

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Hancornia speciosa Gomes (Apocynaceae) is a fruit tree, popularly known as mangabeira, and it is widely distributed throughout Brazil. Several parts of the plant are used in folk medicine, and the leaf and bark extracts have anti-inflammatory, antihypertensive, antidiabetic, and antimicrobial properties. In this study, we investigated the chemical composition of the ethanolic extract of Hancornia speciosa leaves (EEHS) and its antioxidant, antimicrobial, and cytotoxic activities as well as the mechanisms involved in cell death. The chemical compounds were identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). The antioxidant activity of the EEHS was investigated using the method that involves the scavenging of 2,2-diphenyl-1-picrylhydrazyl free radicals as well as the inhibition of oxidative hemolysis and lipid peroxidation induced by 2,2’-azobis (2-amidinopropane) in human erythrocytes. The antimicrobial activity was determined by calculating the minimum inhibitory concentration, minimum bactericidal concentration, minimum fungicidal concentration, and zone of inhibition. Kasumi-1 leukemic cells were used to assess the cytotoxic activity and mechanisms involved in cell death promoted by the EEHS. The chemical compounds identified were quinic acid, chlorogenic acid, catechin, rutin, isoquercitrin, kaempferol-rutinoside, and catechin-pentoside. The EEHS demonstrated antioxidant activity via the sequestration of free radicals, inhibition of hemolysis, and inhibition of lipid peroxidation in human erythrocytes incubated with an oxidizing agent. The antimicrobial activity was observed against American Type Culture Collection (ATCC) and hospital strains of bacteria and fungi, filamentous fungi and dermatophytes. The cytotoxic activity of the EEHS was induced by apoptosis, reduction of the mitochondrial membrane potential, and activation of cathepsins. Together, these results indicate the presence of phenolic compounds and flavonoids in the EEHS and that their antioxidant, antimicrobial, and cytotoxic activities in acute myeloid leukemia cells are mediated by apoptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes

et al. 2016

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“…The large decrease in leaf number in the older plants indicates that the deciduousness typical of the species [29] occurs even in the absence of a water deficit. Considering that the carobinha organs used in the traditional medicine are the leaves and the roots [5] [6], the best time to harvest must be taken into account, since, the older the plant, the higher root production is. On the other hand, leaf production is larger upon regrowth in summer and lower in winter.…”

Section: Effect Of Plantmentioning

confidence: 99%

“…The folk medicine indicates their roots as blood depurative, in the healing of uterine and ovarian wounds, and in the treatment of rheumatic problems and dysentery. Recently reports indicate the anti-inflammatory effect of the carobinha roots and the anti-oxidative, anti-tumoral and anti-obesity activities of its leaves [5] [6] [7].…”

Section: Introductionmentioning

confidence: 99%

Nitrogen and Phosphorus Fertilization Promotes Aerial Part Development and Affect Nutrient Uptake by Carobinha of the Brazilian Cerrado

Gonçalves¹,

Vieira²,

Carnevali³

et al. 2017

AJPS

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Producing Brazilian Cerrado plants, especially ones endangered, is essential for your maintenance. In this way, fertilization is furthermore uncertain. Here, we demonstrate the impact of soil addition of nitrogen (N, 4.20, 18.90, 31.50, 44.10 and 59.85 mg·dm −3 ) and phosphorus (P, 9.56, 57.38, 95.62, 133.86 and 181.67 mg·dm −3 ) fertilizers levels on the development and on nutrients uptake by Jacaranda decurrens subsp. symmetrifoliolata (carobinha), species of the Brazilian Cerrado, in a long term pot trial. The N and P addition together increased plant height and N concentration in roots. N and P also increased the P concentration and content on the roots in young plants, but in the older plants, isolated effect of both was stronger than their combined action. The N addition promoted branching, production of dry leaves and dry xylopodium, contents of K, Ca and P on the leaves, and N content on the roots. However, the N reduced xylopodium diameter, leaf area, and Mg contents in the young plants, but increased them in the older plants. The P addition increased stem diameter and dry biomass, P concentration and N content on the leaves, Ca content on the roots and also reduced N concentration on the leaves. However, the P addition increased Mg concentration on the roots in the young plants and reduced it in the older plants. In general, N levels ranging between 25.69 -38.85 mg·dm −3 and P levels between 84.39 -109.23 mg·dm −3 promote more effectively the plant development. Thus, N and P fertilization can promote the aerial development of plant and a differential allocation of nutrients between the carobinha tissues.

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4‐Deoxyraputindole C induces cell death and cell cycle arrest in tumor cell lines

Vital

Torquato

Jesus

et al. 2018

J of Cellular Biochemistry

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Several molecules extracted from natural products exhibit different biological activities, such as ion channel modulation, activation of signaling pathways, and anti-inflammatory or antitumor activity. In this study, we tested the antitumor ability of natural compounds extracted from the Raputia praetermissa plant. Among the compounds tested, an alkaloid, here called compound S4 (4-Deoxyraputindole C), showed antitumor effects against human tumor lineages. Compound S4 was the most active against Raji, a lymphoma lineage, promoting cell death with characteristics that including membrane permeabilization, dissipation of the mitochondrial potential, increased superoxide production, and lysosomal membrane permeabilization. The use of cell death inhibitors such as Z-VAD-FMK (caspase inhibitor), necrostatin-1 (receptor-interacting serine/threonine-protein kinase 1 inhibitor), E-64 (cysteine peptidases inhibitor), and N-acetyl-L-cysteine (antioxidant) did not decrease compound S4-dependent cell death. Additionally, we tested the effect of cellular activity on adherent human tumor cells. The highest reduction of cellular activity was observed in A549 cells, a lung carcinoma lineage. In this lineage, the effect on the reduction of the cellular activity was due to cell cycle arrest, without plasma membrane permeabilization, loss of the mitochondrial potential or lysosomal membrane permeabilization. Compound S4 was able to inhibit cathepsin B and L by a nonlinear competitive (negative co-operativity) and simple-linear competitive inhibitions, respectively. The potency of inhibition was higher against cathepsin L. Compound S4 promoted cell cycle arrest at G 0 and G 2 phase, and increase the expression of p16 and p21 proteins. In conclusion, compound S4 is an interesting J Cell Biochem. 2019;120:9608-9623. wileyonlinelibrary.com/journal/jcb 9608 |

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Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves

Cited by 32 publications

References 48 publications

Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes

Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes

Nitrogen and Phosphorus Fertilization Promotes Aerial Part Development and Affect Nutrient Uptake by Carobinha of the Brazilian Cerrado

4‐Deoxyraputindole C induces cell death and cell cycle arrest in tumor cell lines

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