Antioxidant and free radical scavenging activity of Pithecellobium dulce (Roxb.) Benth wood bark and leaves

Katekhaye, Shankar; Kale, Maheshkumar S.

doi:10.5530/ax.2012.3.7

Cited by 27 publications

(21 citation statements)

References 35 publications

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“…It is worth noting that ROS in plants is released as normal byproduct in various pathways including photosynthesis. It has been found that every 1–2% of oxygen taken in by plants is inevitably converted to ROS such as perhydroxyl radical, hydroxyl radicals and superoxide radical (Katekhaye and Kale ). Excessive accumulation of ROS results in oxidative damage to cellular and organelle membranes through peroxidation of lipids (Zhang et al ).…”

Section: Discussionmentioning

confidence: 99%

Genome wide identification of the trihelix transcription factors and overexpression of Gh_A05G2067 (GT‐2), a novel gene contributing to increased drought and salt stresses tolerance in cotton

Magwanga

Kirungu

et al. 2019

Physiologia Plantarum

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We identified 102, 51 and 51 proteins encoded by the trihelix genes in Gossypium hirsutum, Gossypium arboreum and Gossypium raimondii, respectively. RNA sequence data and real‐time quantitative polymerase chain reaction analysis showed that Gh_A05G2067 (GT‐2) was highly upregulated under drought and salt stress conditions. Transient expression of GT‐2‐green fluorescent protein fusion protein in protoplast showed that GT‐2 was localized in the nucleus. The overexpression of GT‐2 conferred an enhanced drought tolerance to cotton, with lower malondialdehyde, hydrogen peroxide contents and higher reactive oxygen scavenging enzyme activities. Moreover, chlorophyll content, relative leaf water content (RLWC), excised leaf water loss (ELWL) and cell membrane stability (CMS) were relatively stable in the GT‐2‐overexpressed lines compared to wild‐type (WT). Similarly, stress‐responsive genes RD29A, SOS1, ABF4 and CBL1 were highly upregulated in the GT‐2‐overexpressed lines but were significantly downregulated in WT. In addition, the GT‐2‐silenced cotton plants exhibited a high level of oxidation injury, due to high levels of oxidant enzymes, in addition to negative effects on CMS, ELWL, RLWC and chlorophyll content. These results mark the foundation for future exploration of the trihelix genes in cotton, with an aim of developing more resilient, versatile and highly tolerant cotton genotypes.

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Section: Discussionmentioning

confidence: 99%

Genome wide identification of the trihelix transcription factors and overexpression of Gh_A05G2067 (GT‐2), a novel gene contributing to increased drought and salt stresses tolerance in cotton

Magwanga

Kirungu

et al. 2019

Physiologia Plantarum

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“…They were produced in this study by incubating ferric-EDTA with ascorbic acid and H 2 O 2 at pH 7.4, and reacted with 2-deoxy-2-ribose to generate a malondialdehyde (MDA)-like product. This compound forms a pink chromogen upon heating with TBA at low pH (Katekhaye et al, 2012). When P. dulce, S. longepedunculata and C. sanguinolenta extracts were added to the reaction mixture, they removed the hydroxyl radicals from the sugar and prevented the reaction.…”

Section: Discussionmentioning

confidence: 99%

“…When P. dulce, S. longepedunculata and C. sanguinolenta extracts were added to the reaction mixture, they removed the hydroxyl radicals from the sugar and prevented the reaction. According to Katekhaye et al (2012), the total antioxidant activity (IC 50 values) in DPPH assay by methanol and acetone P. dulce extract showed interesting results. Barku et al, (2012) confirmed that cryptolepine and quindoline compounds were present in the roots and in the stem of Cryptolepis sanguinolenta.…”

Section: Discussionmentioning

confidence: 99%

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Antibacterial and Anti-Inflammatory Activities of Crude Extracts of Three Togolese Medicinal Plants Against Esbl Klebsiella Pneumoniae Strains

Toudji

Thiombiano

Karou³

et al. 2017

Afr J Tradit Complement Altern Med

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Background: Pithecellobium dulce (Roxb.) Benth., Securidaca longepedunculata Fresn and Cryptolepis sanguinolenta (Lindl.) Schlt are three plants widely used in the Togolese traditional medicine to treat microbial infections. Some studies reported their antibacterial activity alone but until know there no data concerning their possible interaction with conventional antibiotics. The main objective of this study was to investigate the antibacterial activity of the association of the crude extracts of the three plants with some conventional antibiotics. We further evaluate the antioxidant and the anti-inflammatory activities of the extracts on rat's model. Materials and methods:The antimicrobial activity was evaluated by the broth microdilution assay and the Fractional inhibitory concentrations (FIC) determined by the checkerboard method. The anti-inflammatory activity was evaluated using the Carrageenan-induced rat paw edema model. The antioxidant activities and the phenol contents were determined by spectrophotometry. Results: The MICs of hydroethanolic extract of plants ranged from 3.125 to 100 mg/mL on Klebsiella pneumoniae strains. Synergistic action was observed only with the combination of Imipenem/P. dulce, imipenem/C. sanguinolenta, amikacin/P. dulce and amikacin/C. sanguinolenta against the ESBL negative Klebsiella pneumoniae strain. Of the 21 associations, 15 were antagonistic on the ESBL-producing strains. The indifference effect was observed with the combination of the extract of Securidaca longepedunculata and the following antibiotics imipenem, amikacin, tetracyclin, ciprofloxacin, Cefotaxim; and Sulfametoxazol+Trimethoprim. The in vitro anti-inflammatory with Lipoxygenase inhibition activity was best with C. sanguinolenta extract while the in vivo paw edema model revealed that S. longepedunculata was the highest reducer of paw edema. In addition white blood cells count and biochemical parameters such as total proteins and immunoglobulins were significantly affected by the administration of plant extracts. Conclusion: This study revealed that the three plants although they may inhibit the bacterial growth by themselves, but there is also a possible synergistic action with the commercial antibiotics. Further investigations are needed to identify the active compounds and their mechanism of action.

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“…Increased free radical production may induce a continuous level of oxidative damage, which leads to the development of many diseases, such as atherosclerosis, cancer, stroke, asthma, arthritis, diabetes mellitus (DM), aging, cardiovascular, inflammatory and neurodegenerative diseases, such as Alzheimer 's disease (AD) . Hence, certain amounts of antioxidant supplements are required to maintain an adequate level of antioxidants in order to balance the reactive oxygen species (ROS) and the reactive nitrogen species (RNS) in human body …”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antioxidant, Anticholinesterase, and Antidiabetic Potential of Dry Leaves and Stems in Tamarix aphylla Growing Wild in Tunisia

Mahfoudhi

Grosso

Gonçalves

et al. 2016

Chemistry & Biodiversity

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Tamarix aphylla (L.) Karst. has a wide geographic distribution and was employed in traditional medicine as astringent, anti-rheumatic and to treat fever. T. aphylla leaves and stems extracts were studied from both chemical and biological points of view to assess the antidiabetic, anticholinesterase and antioxidant potential of this species. The HPLC/Diode Array Detector (DAD) analysis showed the presence of 14 phenolic compounds (gallic, caffeic, p-coumaric, ferulic and ellagic acids, kaempferol, quercetin, quercetin 3-O-galactoside and six flavonol derivatives). This is the first study reporting a comparative study of the biological activities of different extracts from T. aphylla. High activities were obtained against DPPH radical, superoxide anion radical (O2∙-) and nitric oxide radical ( NO) in a concentration-dependent manner, the most active extracts being the polar ones. T. aphylla also showed moderate protective effects against acetylcholinesterase, but no effects were observed against butyrylcholinesterase. Against α-glucosidase the MeOH extracts displayed IC values from 8.41 to 24.81 μg/ml.

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Antioxidant and free radical scavenging activity of Pithecellobium dulce (Roxb.) Benth wood bark and leaves

Cited by 27 publications

References 35 publications

Genome wide identification of the trihelix transcription factors and overexpression of Gh_A05G2067 (GT‐2), a novel gene contributing to increased drought and salt stresses tolerance in cotton

Genome wide identification of the trihelix transcription factors and overexpression of Gh_A05G2067 (GT‐2), a novel gene contributing to increased drought and salt stresses tolerance in cotton

Antibacterial and Anti-Inflammatory Activities of Crude Extracts of Three Togolese Medicinal Plants Against Esbl Klebsiella Pneumoniae Strains

Evaluation of Antioxidant, Anticholinesterase, and Antidiabetic Potential of Dry Leaves and Stems in Tamarix aphylla Growing Wild in Tunisia

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