Antioxidant and genoprotective effects of spent coffee extracts in human cells

Abstract

Abbreviations:8-oxoguanine, 8-oxoGua; DAPI, 4,6-diamidino-2-phenylindole; FPG, formamidopyrimidine DNA glycosylase; H 2 DCF-DA, 2´, 7´-dichlorfluoresceindiacetate; H 2 O 2 , hydrogen peroxide; MTT, (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide); ROS, reactive oxygen species; SBs, strand breaks. 1 AbstractSpent coffee has been shown as a good source of hydrophilic antioxidant compounds.The ability of two spent coffee extracts rich in caffeoylquinic acids, mainly dicaffeoylquinic acids, and caffeine (Arabica filter and Robusta espresso) to protect against oxidation and DNA damage in human cells (HeLa) was evaluated at short (2 h) and long (24 h

Phytochemical Composition, Antioxidant Activity, and the Effect of the Aqueous Extract of Coffee (Coffea arabica L.) Bean Residual Press Cake on the Skin Wound Healing

“…Previous study by Bagdas et al [25] reported that systematical CGA application in skin flap surgeries can accelerate healing and flap survival in animal of wound healing delay by diabetic condition. It has been also suggested that topically CGA ointment has a potent wound healing effect on nondiabetics rats [26]. Thus, our results are consistent with previous reports in literature.…”

In vitrostudies on the stability in the proximal gastrointestinal tract and bioaccessibility in Caco-2 cells of chlorogenic acids from spent coffee grounds

“…Previous studies have highlighted a higher content of antioxidant and bioactive compounds in Arabica and Robusta spent coffee extracts compared with their respective coffee brews (Bravo et al, 2012(Bravo et al, , 2013. Our study demonstrated the high levels of unextracted CGAs in spent Arabica and Robusta coffee compared with the brews, and showed that the aqueous extracts of spent coffee were stable under gastrointestinal conditions and as bioaccessible as the compounds found in coffee beverage.…”

“…Arabica and Robusta coffees are the most consumed varieties. Previously it was shown that the spent coffees obtained after the preparation of a filter (Arabica coffee) and an espresso (Robusta coffee) coffee brew had the highest antioxidant activity in chemical-based assays and in cell cultures (Bravo et al, 2012(Bravo et al, , 2013. Thus, the two spent coffees were used to prepare extracts which were lyophilized, stored, and reconstituted prior to analysis and testing, along with their respective coffee brews.…”

Aflatoxin B1 and aflatoxin M1 induced cytotoxicity and DNA damage in differentiated and undifferentiated Caco-2 cells

“…Cell viability was determined by assessing the reduction of MTT to formazan by the mitochondrial enzyme as described (Bravo et al, 2013). Stock solutions of mycotoxins were prepared in methanol.…”