Antioxidant and hepatoprotective potential of Lawsonia inermis L. leaves against 2-acetylaminofluorene induced hepatic damage in male Wistar rats

Abstract

Background Lawsonia inermis (Lythraceae) is an ethnomedicinal plant, traditionally known for curing several ailments such as skin diseases, bacterial infections, jaundice, renal lithiases and inflammation etc. The present work deals with assessment of in vitro antioxidant and in vivo hepatoprotective potential of butanolic fraction (But-LI) of Lawsonia inermis L. leaves.MethodsAntioxidant activity was evaluated using deoxyribose degradation, lipid peroxidation inhibition and ferric reducing antioxidant power (FRAP) assay. In vivo protective potential of But-LI was assessed at 3 doses [100, 200 & 400 mg/kg body weight (bw)] against 2-acetylaminofluorene (2-AAF) induced hepatic damage in male Wistar rats.ResultsBut-LI effectively scavenged hydroxyl radicals in deoxyribose degradation assay (IC50 149.12 μg/ml). Fraction also inhibited lipid peroxidation and demonstrated appreciable reducing potential in FRAP assay. Treatment of animals with 2-AAF resulted in increased hepatic parameters such as SGOT (2.22 fold), SGPT (1.72 fold), ALP (5.68 fold) and lipid peroxidation (2.94 fold). Different concentration of But-LI demonstrated pronounced protective effects via decreasing levels of SGOT, SGPT, ALP and lipid peroxidation altered by 2-AAF treatment. But-LI administration also restored the normal liver architecture as evident from histopathological studies.ConclusionsThe present experimental findings revealed that phytoconstituents of Lawsonia inermis L. possess potential to effectively protect rats from the 2-AAF induced hepatic damage in vivo possibly by inhibition of reactive oxygen species and lipid peroxidation.

Effect of Pretreatment with Aqueous Leaf Extract of Vitex doniana on Cadmium-Induced Toxicity to Rats

“…The results of this study showed that exposure to cadmium causes a significant increase in membrane lipid peroxidation in the liver and kidneys of rats but pretreatment with aqueous leaves extract of Vitex doniana was effective in the prevention of oxidative damage induced by cadmium, which resulted in significantly lower degree of lipid peroxidation in the liver and kidneys. This is consistent with the previous studies in which natural plant products effectively reduced lipid peroxidation induced in response to various toxicants [36,37,38]. This can be explained by the important role of polyphenols in preventing lipid peroxidation and in the protection of the integrity and functioning of tissues and cells.…”

Antioxidant, Anti-Inflammatory and Antidiabetic Proprieties of LC-MS/MS Identified Polyphenols from Coriander Seeds

“…The liver enzymes, aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), are routinely used to evaluate liver function; they are considered markers of hepatocellular health [ 34 ]. The elevated level of these enzymes is a marker of liver pathology and might be attributed to Alloxan’s action [ 35 ].…”

Lawsonia inermis essential oil: extraction optimization by RSM, antioxidant activity, lipid peroxydation and antiproliferative effects

“…Recently, Kumar et al [40] have shown following a thiobarbituric lipid peroxidation inhibition test (TBARS) of the butanolic fraction of Lawsonia inermis leaves (But-LI), a moderate inhibition of 58.90% at the maximum concentration (1000 μg/mL) and 39.33% at the lowest concentration (100 μg/mL). The But-LI fraction had a higher IC50 (375.73 μg/mL) than the standard Trolox (IC50 = 136.47 μg/mL).…”