Antioxidant and immune-enhancing effects of probiotic Lactobacillus plantarum 200655 isolated from kimchi

Abstract

Probiotic properties including antioxidant and immune-enhancing effects of Lactobacillus plantarum 200655 isolated from kimchi were evaluated. The tolerance of three strains (L. plantarum 200655, L. plantarum KCTC 3108, and L. rhamnosus GG to bile salts (0.3% oxgall, 24 h) was similar, and L. plantarum 200655 showed the highest tolerance to gastric juice (0.3% pepsin, 3 h). All strains presented similar autoaggregation ability. L. plantarum 200655 showed higher cell surface hydrophobicity and adhesion ability on HT-29 cells. L. plantarum 200655 did not produce b-glucuronidase and was sensitive to ampicillin, tetracycline, chloramphenicol, and doxycycline. Additionally, L. plantarum 200655 showed the highest antioxidant effects in DPPH and ABTS radical scavenging, and b-carotene bleaching assays. RAW 264.7 cells treated with L. plantarum 200655 produced more nitric oxide, induced nitric oxide synthase, and cytokine related to immune-enhancing effects such as interleukin-1b and interleukin-6. Therefore, L. plantarum 200655 could be useful as a probiotic strain for older people.

Probiotic Properties and Neuroprotective Effects of Lactobacillus buchneri KU200793 Isolated from Korean Fermented Foods

“…All tested LAB strains were resistant to gentamycin, kanamycin, streptomycin, and ciprofloxacin; however, they were susceptible to ampicillin, tetracycline, chloramphenicol, and doxycycline (Table 3). These results are acceptable based on the Clinical and Laboratory Standards Institute guideline [2,27].…”

“…To measure the tolerance of the strains for the gastric conditions, the experiment was performed according to a previous method [2]. The strains were incubated in MRS broth at 37 • C for overnight.…”

“…The radical scavenging activity of 2,2 -azinobis (2-ethylbenzothiazoline-6-sulfonate) (ABTS) was measured as described by a previous method with some modifications [2]. To prepare ABTS solution, 7 mM ABTS and 5 mM potassium persulfate was mixed with 20 mM sodium phosphate buffer (pH 7.4), and they were allowed to react at room temperature in the dark for 16 h. Using 20 mM sodium phosphate buffer, ABTS + solution was diluted until the final absorbance changed to 0.7 ± 0.02 at 734 nm.…”

“…Probiotics are defined as living microorganisms administered in appropriate amounts that confer a beneficial effect on the host [1]. To be used as probiotics, microorganisms should have the capacity to withstand physical and chemical conditions in the human body and to colonize and adhere to the intestinal epithelial cells [2]. Some probiotics, known as brain probiotics, produce gamma-aminobutyric acid and serotonin [3,4].…”

Probiotic Properties of Lactobacillus brevis KU200019 and Synergistic Activity with Fructooligosaccharides in Antagonistic Activity against Foodborne Pathogens

“…(v/v) fetal bovine serum (Hyclone), and 1% (v/v) penicillin-streptomycin (Hyclone) at 37℃ in a humidified atmosphere containing 5% CO 2 . The adherence ability of L. brevis strains to HT-29 cell lines was performed as described previously (Yang et al, 2019). Aliquot of bacterial suspension 1×10 8 CFU/mL was added to confluent HT-29 monolayers and subsequently, supplemented with prebiotics (20 mg/mL) and incubated at 37℃ for 2 h. Samples either added with L. brevis strains or prebiotics were considered as control samples.…”