Antioxidant and immunomodulatory activities of polysaccharides from the roots of Sanguisorba officinalis

Zhang, Lin; Koyyalamudi, Sundar Rao; Jeong, Sang-Chul; Reddy, N. Venkata; Smith, Paul T.; Ananthan, R.; Longvah, T.

doi:10.1016/j.ijbiomac.2012.08.019

Cited by 70 publications

(43 citation statements)

References 37 publications

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“…Sanguisorbae Radix has been reported that it possesses some beneficial effects such as anti-cancer [13], anti-inflammation [5, 14], anti-oxidation [15], neuroprotection [16, 17] and renal protection [18]. Currently, we have reported the inhibitory action of its ethanol extract on 2, 4-dinitrochlorobenzene (DNCB)-induced AD model [19].…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Yang

Yoo

Cho

et al. 2016

BMC Complement Altern Med

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BackgroundSanguisorbae Radix (SR) is a well-known herbal medicine used to treat inflammatory disease and skin burns in Asia. In addition, it is used to treat many types of allergic skin diseases, including urticaria, eczema, and allergic dermatitis. SR has been reported to exhibit anti-wrinkle, anti-oxidant, and anti-contact dermatitis bioactivities.MethodsIn this study, we investigated the mechanism underlying the anti-inflammatory effects of SR water extract (WSR) using human keratinocyte (HaCaT) cells and BALB/c mouse bone marrow-derived mast cells (BMMCs). Viability assays were used to evaluate non-cytotoxic concentrations of WSR in both BMMCs and HaCaT cells. To investigate the effect of WSR treatment on the degranulation of IgE/Ag-activated BMMCs, we measured the release of β-hexosaminidase (β-HEX). We determined the production of pro-inflammatory chemokines including thymus and activation regulated chemokine (TARC; CCL17), regulated on activation, normal T-cell expressed and secreted (RANTES; CCL5), macrophage-derived chemokine (MDC; CCL22), and interleukin 8 (IL-8; CXCL8) in stimulated human keratinocytes. The ability of WSR to reduce the expression of pro-inflammatory marker proteins was evaluated by Western blotting in HaCaT cells stimulated with tumor necrosis factor (TNF)-α/interferon (IFN)-γ.ResultWSR inhibited IgE/Ag-activated mast cell degranulation in BMMCs. Treatment with various concentrations of WSR decreased β-HEX release in a dose-dependent manner with an IC50 of 27.5 μg/mL. In keratinocytes, WSR suppressed TNF-α/IFN-γ-induced chemokine production and pro-inflammatory molecules via a blockade STAT-1, Jak-2, p38, and JNK activation.ConclusionsThis results demonstrate that WSR inhibits degranulation of IgE/Ag-activated mast cells and inhibits the production of pro-inflammatory chemokines by suppressing the phosphorylation of p38 and JNK in HaCaT cells.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Yang

Yoo

Cho

et al. 2016

BMC Complement Altern Med

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“…DPPH scavenging assay is widely used to examine antioxidant activity since it is sensitive, simple, and rapid [15]. Hydroxyl radical is one of the most powerful oxidative species [16]. The last line of defense barrier in animals is the immune system, which resists inflammation, infection, and cancer cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

Optimized Extraction, Preliminary Characterization, and In Vitro Antioxidant Activity of Polysaccharides from Glycyrrhiza Uralensis Fisch

Chen

2017

Med Sci Monit

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BackgroundThis study performed optimized extraction, preliminary characterization, and in vitro antioxidant activities of polysaccharides from Glycyrrhiza uralensis Fisch.Material/MethodsThree parameters (extraction temperature, ratio of water to raw material, and extraction time) were optimized for yields of G. uralensis polysaccharides (GUP) using response surface methodology with Box-Behnken design (BBD). The GUP was purified using DEAE cellulose 32-column chromatography. The main fraction obtained from G. uralensis Fisch was GUP-II, which was composed of rhamnose, arabinose, galactose, and glucose monosaccharide, was screened for antioxidant properties using DP Hand hydroxyl radical scavenging assays. In addition, immunological activity of GUP-II was determined by nitric oxide and lymphocyte proliferation assays.ResultsOptimization revealed maximum GUP yields with an extraction temperature of 99°C, water: raw material ratio of 15: 1, and extraction duration of 2 h. GUP-II purified from G. uralensis Fisch had good in vitro DPPH and hydroxyl radical scavenging abilities. Immunologically, GUP-II significantly stimulated NO production in RAW 264.7 macrophages, and significantly enhanced LPS-induced lymphocyte proliferation.ConclusionsExtraction of GUP from G. uralensis Fisch can be optimized with respect to temperature, extraction period, and ratio of water to material, using response surface methodology. The purified product (GUP-II) possesses excellent antioxidant and immunological activities.

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“…Statistical analyses were performed to compare the treated groups with the respective control group using a one-way analysis of variance (ANOVA) complemented with the Tukey-Kramer multiple comparison test with equal sample size. Computations were performed by employing the statistical software (SPSS, version 20.0) (18).…”

Section: Rt-qpcr Detection Hela Apoptotic Gene Expressionmentioning

confidence: 99%

Induction and mechanism of HeLa cell apoptosis by 9-oxo-10, 11-dehydroageraphorone from Eupatorium adenophorum

Liao

et al. 2015

Oncology Reports

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Abstract. 9-Oxo-10, 11-dehydroageraphorone (euptox A), a cadenine sesquiterpene, is the main toxin from Eupatorium adenophorum. The aim of the present study was to examine the induction and mechanism of HeLa cell apoptosis by euptox A. The apoptosis-inducing effect of the euptox A on HeLa cells was examined by MTT assay. The underlying mechanism was analyzed by flow cytometry and quantitative PCR. Flow cytometry results suggested that euptox A effectively inhibited the proliferation of HeLa cells, arrested the cell cycle transition from S to G2/M phase, did not continue to complete the cell cycle activity (mainly from 4 times and mitosis), and induced cell proliferation. The RT-qPCR detection results showed that euptox A induced apoptosis by improving the gene expression level of apoptotic proteases such as caspase-10 in HeLa cells. Its mechanism of action was associated with the upregulation of apoptotic gene expression and arresting of the cell cycle. IntroductionEupatorium adenophorum (E. adenophorum), native to Mexico and Costa Rica of Central America, is a worldwide noxious invasive weed (1). After its introduction as an ornamental plant to the USA in the 1960s, it has spread worldwide (2), as a non-native species to India, New Zealand, and Australia. In China, it first invaded southern regions of Yunnan Province from Burma in the 1940s (3). At present, E. adenophorum can be found in Chongqing, Yunnan, Sichuan, Guizhou, Tibet, Guangxi, Taiwan and Hubei provinces. The annual spreading rate of E. adenophorum is estimated to be 10-60 km from south to north and from west to east in China (4). It is considered a threat to the local economy and biodiversity. However, as has been reported, several compounds have been separated and characterized from the E. adenophorum stem, flowers and leaves, including hemiterpenes, sterides, triterpenes, flavonoid and phenylpropanoids phenol, which have extensive biological activity, such as anti-inflammatory potential (5), acaricidal (6,7) and antioxidant activity (8). E. adenophorum can be used as a food (9), medical (10), and chemical material resource (11).9-Oxo-10, 11-dehydroageraphorone (euptox A), a cadenine sesquiterpene, is the main toxin from E. adenophorum. Results of previous studies have shown that the euptox A from E. adenophorum exhibited hepatotoxicity (12) and allelopathy (13). Our laboratory has proved that euptox A exhibited highly acaricidal activity for S. scabiei and P. cuniculi in vitro (14) and euptox A presented significantly antitumor activity against the human lung cancer A549, HeLa and Hep-2 cell lines in vitro in a dose-dependent manner (15).In the present study, MTT, flow cytometry and RT-PCR were used to detect the change of indices prior to and following drug action, followed by a comparison of the availability of different methods. Materials and methodsMaterials. Euptox A was provided by the laboratory of biotoxin and molecular toxicology of Sichun Agriculture University, China. The purity of the toxin we extracted was >96% (16).Cell cultures. ...

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Antioxidant and immunomodulatory activities of polysaccharides from the roots of Sanguisorba officinalis

Cited by 70 publications

References 37 publications

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Anti-inflammatory effects of Sanguisorbae Radix water extract on the suppression of mast cell degranulation and STAT-1/Jak-2 activation in BMMCs and HaCaT keratinocytes

Optimized Extraction, Preliminary Characterization, and In Vitro Antioxidant Activity of Polysaccharides from Glycyrrhiza Uralensis Fisch

Induction and mechanism of HeLa cell apoptosis by 9-oxo-10, 11-dehydroageraphorone from Eupatorium adenophorum

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