Antioxidant and Immunomodulatory Activity of the Alkaloidal Fraction of Cissampelos pareira Linn.

Abstract

The alkaloidal fraction (AFCP) of roots of Cissampelos pareira Linn. was screened for in-vitro antioxidant activity and immunomodulatory activity in mice. The HPTLC finger print profile was also established for the identification of AFCP which was found to contain 0.176 % of berberine. AFCP possess strong antioxidant activity which was revealed by its ability to scavenge the stable free radical DPPH, superoxide ion and to inhibit lipid peroxidation in rat liver homogenate induced by iron/ADP/Ascorbate complex. AFCP was found to have significant immunosuppressive activity at lower doses (25 and 50 mg/kg) while no activity was observed at higher doses (75 and 100 mg/kg). Humoral antibody titre was significantly (p<0.01) lowered by AFCP at the doses of 25 and 50 mg/kg. Delayed type hypersensitivity response was also significantly (p<0.01) suppressed by the AFCP at the dose of 75 mg/kg. Thus the present study revealed the immunosuppressive and antioxidant activities of the alkaloidal fraction of C. pareira roots.

Immunostimolatory activities ofVigna mungoL. extract in male Sprague–Dawley rats

“…The settled SRBC were then suspended in normal saline. The SRBC in this suspension were adjusted to a concentration of 5 × 10 9 SRBC/mL for immunization and challenge (Bafna and Mishra, 2005).…”

Diuretic activity of aqueous extract of roots of &lt;i&gt;Cissampelos pareira&lt;/i&gt; in albino rats

“…The plant C. pareira was also reported with berberine. 13 When tested for diuretic activity, berberine 22 increased urine excretion in the rats. Increase in the urinary volume was also accompanied by an increase in the Na + , K + excretion similar to the standard diuretic hydrochlorthiazide, suggesting that berberine 22 induced diuresis is caused by its saluretic effect.…”

“…Some scientific studies revealed its antinociceptive, 5 antiarthritic, 5 cardiotonic, 6 anticancer, 7 anti-inflammatory, 8 antidiarrheal, 9 anti-hemorrhagic, antifertility, 10 antioxidant, neuroprotective, 11 hepatoprotective, 12 antioxidant, 13 immunomodulatory, 13 anti trypanosomal activities.The major constituents of roots of C. pareira include 14 Pelosin, O-methylcurine, l-curine Cissamine, Cissampareine, Hyatin, Bebeerine, Cycleanine, Tetrandine and Beriberine, Cissampeline, Cissampoline, Dicentrine, Insularine, Pareirine, Hyatinine, Pareirubrine A, Pareirubrine B, Pareitropone, Norimeluteine, Cissampeloflavone, D-Quercitol and Grandirubrine. 14 The roots of C. pareira traditionally used as a diuretic but scientifically not evaluated as a diuretic agent.…”

A Review on Medicinal Plants Used as Diuretics From Karnataka State

“…Anti-diarrhoeal activity [86] , Gastroprotective activity [87] , Chemo-preventive effects [88] , Anti-hyperglycemic activity [89] , Antioxidant and immunomodulatory activity [90] , Anti-inflammatory activity [91,92] Anti-fertility activity [93,94] , Memory enhancing activity [95] , Anti-nociceptive and Antiarthriritic activity [96] , Cardioprotective activity [97] , Anti-oxidant activity [98] Anti-haemorrhagic effects [99] , Hepato-protective activity [100] 3.…”