This study evaluates the antioxidant, immune-enhancing, and anti-diabetic activities of Allium hookeri leaves (AHL) and roots (AHR) grown in an outfield (S) and in a plant factory system using fluorescent lamp (F) and light-emitting diode (blue+red+white LED; L) lights. The AHL and AHR extracts were prepared using 50% ethanol, and the cycloalliin concentrations of all extracts were measured by LC/MS. Antioxidant effects were determined by evaluating the total phenolic content (TPC) and DPPH-and ABTS-radical scavenging activities. The immune-enhancing activity was determined by measuring the nitric oxide (NO) content produced from RAW264.7 cells. The α-amylase and α-glucosidase inhibitory activities were examined to investigate the anti-diabetic effect of the extracts. Our results revealed higher amounts of TPC in AHL-S and AHR-L, whereas AHL-F and AHR-F, and AHL-F and AHR-L exhibited higher DPPH and ABTS radical scavenging activity, respectively. No cytotoxicity was observed for all the samples evaluated. The highest NO concentration was determined in AHL-S and AHR-L extracts. Furthermore, higher α-amylase and α-glucosidase inhibitory activities were observed in AHL-F and AHR-L than in other AHL and AHR extracts. These results suggest that artificial lights potentially improve the functionality of AHL and AHR grown in the plant factory, and the resultant extracts can be used as functional food sources.