Antioxidant and oxidant status, DPPH activity, total phenolic and flavonoid contents of mountain tea (Sideritis libanotica subsp. kurdica (Bornm.) Hub.-Mor)

Abstract

Throughout history, people have found several uses for plants. Numerous research have demonstrated the widespread application of plants as a kind of supplementary therapy. Samples of Sideritis libanotica subsp. kurdica (Bornm.) Hub.-Mor, often known as mountain tea, were gathered in Iraq and analysed for their antioxidant and oxidant status, DPPH activity, total phenolic and total flavonoid contents. The plant's aerial parts were utilised for this purpose. A soxhlet was used to get the plant's ethanol extract. The levels of antioxidants and free radicals were measured by Rel assay kits. The ability to scavenge free radicals was evaluated using the DPPH technique. The total phenolic content was calculated using the Folin-Ciocalteu reagent. Aluminum chloride analysis was used to calculate the total flavonoid content. The results showed that the aerial parts of the plant had TAS values of 7.934±0.179 mmol/L, TOS values of 10.626±0.275 µmol/L, and OSI values of 0.134±0.001. The total phenolic and flavonoid contents were found to be 129.75±2.37 and 111.47±3.15 mg/g, respectively. Furthermore, DPPH activity at 2 mg/mL was calculated to be 75.15±1.45. S. libanotica subsp. kurdica has been found to have antioxidant activity and is thus a viable natural antioxidant source in this context.