Antioxidant and reactive oxygen species scavenging properties of cellular albumin in HepG2 cells is mediated by the glutathione redox system

Abstract

This study was carried out to examine the role of intracellular albumin in the modulation of oxidative damage induced by glutathione modifiers in HepG2 cells. Also, the relationship of albumin synthesis with oxidative stress factors including antioxidants was studied. HepG2 cell culture was supplemented with glutathione modifiers; L-Buthionine-sulfoximine (BSO; 0.1 and 1.0 mM) or N-acetyl cysteine (NAC; 1 and 10 mM) and the cell viability and changes in reduced glutathione (GSH), oxidized glutathione (GSSG), reactive oxygen species (ROS), catalase, and superoxide dismutase were measured. Besides, albumin expression at protein and mRNA levels was determined in cells pretreated with BSO or NAC. Kinetic studies showed that albumin expression in HepG2 cells is correlated with GSH and GSSG levels. Changes in albumin expression at protein and mRNA levels reached their maximum (19% and 55%, respectively) in the cells 6 H after NAC treatments. A substantial decrease in intracellular albumin due to BSO (27%) was associated with a significant increase in the generation of cellular ROS (17%). In contrast, increased albumin synthesis (intracellular and secretory) was associated with inhibition in cellular ROS. Overall data may suggest that albumin expression in coordination with the glutathione redox system is part of the antioxidant defense mechanism in liver cells. C 2018

Drp1 regulates mitochondrial dysfunction and dysregulated metabolism in ischemic injury via Clec16a-, BAX-, and GSH- pathways

“…7I). The results showed the increase in ROS and decrease in △Ψm could be inhibited by supplementing with GSH (10 mM) after hypoxia 35 . However, the effect of Drp1 shRNA treatment on mitochondrial function was more pronounced after hypoxia.…”

“…However, the effect of Drp1 shRNA treatment on mitochondrial function was more pronounced after hypoxia. We also used GSH inhibitor buthionine sulfoxamine (BSO) (1 mM) 35,36 on the basis of Drp1 shRNA treatment and found that the ROS level increased by 28% and △Ψm decreased by 20% ( Fig. 7I-J).…”

Extracellular Matrix Optimization for Enhanced Physiological Relevance in Hepatic Tissue-Chips

“…It can be inferred that ECM composition does not directly affect the albumin and urea release of hepatocytes in a dynamic cell culture environment. It has been found that intracellular albumin has superior diagnostic value, and its concentration may differ for extracellular albumin [ 34 ]. To determine the impact of ECM composition on intracellular albumin expression, hepatocytes were stained for intracellular localization of albumin via immunofluorescence staining.…”

Neural stem cells neuroprotection by simvastatin via autophagy induction and apoptosis inhibition

“…Oxidative stress damages the nerve cells in many neurodegenerative and other diseases (17)(18)(19)(20). Attenuation of oxidative stress increases the survival of NSCs after transplantation into the affected area of the central nervous system.…”