Antioxidant and skin pores contraction activity of Malus domestica Borkh

Abstract

Green apple is important not only for its high antioxidant contents, but also for its acceptance among the general consumer. The aim of this study is to confirm the effect of green apple extracts on skin pores contraction through antioxidant activity, quantification of tannin contents, protein aggregation assay and inhibition of 5‐alpha‐reductase type 1 mRNA expression. First, Green apple was separated to whole apple, apple rind, pulp, seed and each group was extracted with 70% ethanol. Apple rind exhibited significantly higher antioxidant activity, tannin contents. So, apple rind was fractionated with hexane, CHCl3, EtOAc, BuOH and water to get active fraction. Among these solvent fractions, green apple rind ethyl acetate (AR‐E) has a greatest antioxidant activity and tannin content. Also, protein aggregation of green apple fraction has an effect as compared with positive control tannic acid. The major factor contributing to excess sebum is 5‐alpha reductase type1 which related to enlarge skin pores. We confirmed that green apple fraction reduced 5‐alpha reductase type1 expression in HaCaT using RT‐PCR method. In conclusion, we expected the beneficial effect of green apple on skin pores contractionGrant Funding Source : Ipeeres Ltd.