Antioxidant and α-glucosidase inhibitory activities of constituents from Euonymus alatus twigs

Choi, Chang‐Ik; Lee, Seoung Rak; Kim, Ki‐Hyun

doi:10.1016/j.indcrop.2015.08.031

Cited by 39 publications

(26 citation statements)

References 34 publications

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“…Intestinal α‐Gly and pancreatic α‐amylase are important enzymes in catalyzing the first stage in the digestion of starch and in the digestive system, hydrolyzing the α‐1,4‐glucoside linkages . The inhibition of these key enzymes significantly reduces the uptake of carbohydrates and digestion, thereby reducing the postprandial blood glucose amount in the non‐insulin‐dependent diabetes type mellitus patients .…”

Section: Discussionmentioning

confidence: 99%

The impact of some natural phenolic compounds on carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase, and α‐glycosidase enzymes: An antidiabetic, anticholinergic, and antiepileptic study

Taslimi

Çağlayan

Gülçın

2017

J Biochem & Molecular Tox

144

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Natural products from food and plant sources have been used for medicinal usage for ages. Also, natural products with therapeutic significance are compounds derived from animals, plants, or any microorganism. In this study, chrysin, carvacrol, hesperidin, zingerone, and naringin as natural phenols showed excellent inhibitory effects against human (h) carbonic anhydrase (CA) isoforms I and II (hCA I and II), α-glucosidase (α-Gly), acetylcholinesterase (AChE), and butyrylcholinesterase (BChE). These phenolic compounds were tested for the inhibition of α-glycosidase, hCA I, hCA II, AChE, and BChE enzymes and demonstrated efficient inhibition profiles with K values in the range of 3.70 ± 0.92-79.66 ± 20.81 nM against hCA I, 2.98 ± 0.33-84.88 ± 40.32 nM against hCA II, 4.93 ± 2.01-593.60 ± 134.74 nM against α-Gly, 0.52 ± 0.18-46.80 ± 17.15 nM against AChE, and 1.25 ± 0.22-32.08 ± 2.68 against BChE.

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Section: Discussionmentioning

confidence: 99%

The impact of some natural phenolic compounds on carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase, and α‐glycosidase enzymes: An antidiabetic, anticholinergic, and antiepileptic study

Taslimi

Çağlayan

Gülçın

2017

J Biochem & Molecular Tox

144

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“…The amount of p-NP released was measured in an EPOCH 2 (BIOTEK ® , Biotek Instruments Inc., Winooski, VT, USA) microplate reader at 405 nm for 60 min, recording the absorbance every 5 min. The results were expressed as inhibition percentage by means of the formula described by [ 50 ] as follows:

where Ao is the absorbance recorded for the enzymatic activity without inhibitor (control), and As is the absorbance recorded for the enzymatic activity in presence of the inhibitor (sample). IC 50 value was calculated by curve fitting of data (GraphPad Prism 5.0, GraphPad Software, Inc., La Jolla, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

α-Glucosidase Inhibition and Antibacterial Activity of Secondary Metabolites from the Ecuadorian Species Clinopodium taxifolium (Kunth) Govaerts

et al. 2018

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The phytochemical investigation of both volatile and fixed metabolites of Clinopodium taxifolium (Kunth) Govaerts (Lamiaceae) was performed for the first time. It allowed the isolation and characterization of the essential oil and six known compounds: carvacrol (1), squalane (2), uvaol (3), erythrodiol (4), ursolic acid (5), and salvigenin (6). Their structures were identified and characterized by Nuclear Magnetic Resonance (NMR) and Gas Chromatography coupled to Mass Spectroscopy (GC-MS), and corroborated by literature. The essential oil of the leaves was obtained by hydrodistillation in two different periods and analyzed by GC-MS and GC coupled to Flame Ionization Detector (GC-FID). A total of 54 compounds were detected, of which 42 were identified (including trace constituents). The major constituents were carvacrol methyl ether (18.9–23.2%), carvacrol (13.8–16.3%) and, carvacryl acetate (11.4–4.8%). The antibacterial activities were determined as Minimum Inhibition Concentration (MIC) against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa and Micrococcus luteus. The hexane and methanol extracts exhibited activity only against Klebsiella pneumoniae (250 and 500 μg/mL respectively), while the ethyl acetate extract was inactive. The hypoglycemic activity was evaluated by the in vitro inhibition of α-glucosidase. The ethyl acetate (EtOAc) extract showed strong inhibitory activity with IC50 = 24.88 µg/mL, however methanolic and hexanic extracts showed weak activity. As a pure compound, only ursolic acid showed a strong inhibitory activity, with IC50 = 72.71 μM.

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“…Type 2 diabetes mellitus (T2DM) disease is an intense endocrine metabolic dysfunction creating postprandial hyperglycemia, which causes difficult long‐term disorders, such as diabetic retinopathy, diabetic neuropathy, cardiovascular disease, and diabetic nephropathy . α‐Glycosidase and α‐amylase, as the carbohydrate‐hydrolyzing enzyme, are accountable for the ultimate stage in the digestion of dietary glycogen and starch, also α‐amylase and α‐glycosidase inhibitors are drug candidates, as well as potential functional food factors for deferring postprandial absorbency of glucose …”

Section: Discussionmentioning

confidence: 99%

Synephrine and phenylephrine act as α‐amylase, α‐glycosidase, acetylcholinesterase, butyrylcholinesterase, and carbonic anhydrase enzymes inhibitors

Taslimi

Akıncıoğlu

Gülçın

2017

J Biochem & Molecular Tox

125

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In this paper, synephrine and phenylephrine compounds showed excellent inhibitory effects against human carbonic anhydrase (hCA) isoforms I and II, α-amylase, α-glycosidase, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE). Synephrine and phenylephrine had K values of 199.02 ± 16.01 and 65.01 ± 5.00 μM against hCA I and 336.02 ± 74.01 and 92.04 ± 18.03 μM against hCA II, respectively. On the other hand, their K values were found to be 169.10 ± 80.03 and 88.03 ± 5.01 nM against AChE and 177.06 ± 6.01 and 78.03 ± 3.05 nM against BChE, respectively. α-Amylase and α-glycosidase enzymes were easily inhibited by these compounds. α-Glycosidase inhibitors, generally defined to as starch blockers, are anti-diabetic drugs that help to decrease post comestible blood glucose levels.

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Antioxidant and α-glucosidase inhibitory activities of constituents from Euonymus alatus twigs

Cited by 39 publications

References 34 publications

The impact of some natural phenolic compounds on carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase, and α‐glycosidase enzymes: An antidiabetic, anticholinergic, and antiepileptic study

The impact of some natural phenolic compounds on carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase, and α‐glycosidase enzymes: An antidiabetic, anticholinergic, and antiepileptic study

α-Glucosidase Inhibition and Antibacterial Activity of Secondary Metabolites from the Ecuadorian Species Clinopodium taxifolium (Kunth) Govaerts

Synephrine and phenylephrine act as α‐amylase, α‐glycosidase, acetylcholinesterase, butyrylcholinesterase, and carbonic anhydrase enzymes inhibitors

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