Antioxidant, anti-diabetic, acetylcholinesterase inhibitory potential and estimation of alkaloids (lycorine and galanthamine) from Crinum species: An important source of anticancer and anti-Alzheimer drug

Ghane, Savaliram G.; Attar, U. A.; Yadav, Pradnya B.; Lekhak, Manoj M.

doi:10.1016/j.indcrop.2018.08.087

Cited by 77 publications

(45 citation statements)

References 52 publications

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“…Alpha-amylase inhibitors (AAI) are playing a significant role in the treatment of diabetes 28 . The α-amylase enzyme is responsible for breakdown of oligo and/or disaccharide into monosaccharides.…”

Section: α-Amylase Inhibition Activity Diabetes Is One Of the Main Smentioning

confidence: 99%

“…In contrast, aqueous extract of Kedrostis africana tuber represented 31.64 ± 1.11% α-amylase inhibitory activity at 200 mg/mL extract concentration 31 . α-glucosidase inhibitory activity. The deficiency of insulin secretion causes type 2 diabeties which results in hyperglycemia, physical and mental burden and subsequnt increase in the rate of mortality and disability 28 . α-Glucosidase enzyme is responsible for release of glucose by hydrolyzing linear and branched iso-maltose oligosaccharides which cause postprandial hyperglycemia.…”

Section: α-Amylase Inhibition Activity Diabetes Is One Of the Main Smentioning

confidence: 99%

“…DPPH radical scavenging assay. Extracts were analyzed to evaluate DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity as per our earlier protocols 2,3,17,22,28 . The working solution DPPH reagent (1 mL) was mixed with 500 μL of plant extract (1 mg/mL).…”

Section: Antioxidant Activitiesmentioning

confidence: 99%

“…ABTS radical scavenging assay. ABTS [2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)] radical cation decolorization assay was used with slight modifications 2,3,17,22,28 . ABTS solution (1 mL) was treated with 100 µL of plant extract and incubated at 30 °C.…”

Section: Antioxidant Activitiesmentioning

confidence: 99%

“…Phosphomolybdate assay (PMA). PMA assay was performed as per earlier protocols 3,17,22,28 , in which fruit extract (300 μL) was treated with 1 mL phosphomolybdate reagent and incubated at 95 °C for 90 min, cooled and absorbance was recorded at 765 nm. Ascorbic acid was used to obtain a calibration curve.…”

Section: Antioxidant Activitiesmentioning

confidence: 99%

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Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Patel

Attar

Sakate

et al. 2020

Sci Rep

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Diplocyclos palmatus (L.) C. Jeffrey is an important medicinal plant used in several reproductive medicines. It serves as a wide source of tetracyclic triterpens called cucurbitacins. Response surface methodology (RSM) with Box-Behnken design (BBD) was studied to optimize the production of cucurbitacins. RSM put forth the ideal conditions such as 1:30 SS ratio (g/mL), 80 rpm (mixing extraction speed), 150 µm mean particle size, 30 min extraction time and 50 °C using chloroform in continuous shaking extraction (CSE) and showed the highest cucurbitacin I (CUI) content (2.345 ± 0.1686 mg/g DW). Similarly, the highest yield of cucurbitacin B (CUB) (1.584 ± 0.15 mg/g DW) was recorded at ideal conditions (1:40 g/mL SS ratio and 60 min time and others similar to CUI). Among the tested extraction methods, the highest CUI, CUB, and CUI + B yield (1.437 ± 0.03, 0.782 ± 0.10, 2.17 ± 0.35 mg/g DW, respectively) as well as promising DPPH radical scavenging activity (25.06 ± 0.1 µgAAE/g DW) were recorded from the SBAE (steam bath assisted extraction). In addition, MAE and UAE revealed the highest inhibition of α-amylase (68.68%) and α-glucosidase (56.27%) enzymes, respectively. Fruit extracts showed potent anticancer activity against breast (MCF-7) and colon (HT-29) cancer cell lines (LC 50 -44.27 and 46.88 µg/mL, respectively). Our study proved that SS ratio, particle size and temperature were the most positively influencing variables and served to be the most efficient for the highest recovery of CUI and CUB. Based on the present study, the fruits of D. palmatus were revealed as a potent antioxidant, anti-diabetic and anticancer bio-resource that could be explored further to develop novel drug to manage diabetes, cancer and oxidative stress related disorders.The plants from the family Cucurbitaceae are commonly called as melons, squashes, and gourds which are traditionally used in the human diet. It comprises 122 genera and 940 species of which 31 genera and 94 species are found in India 1 . Diplocyclos palmatus (L.) C. Jeffrey is a slender-stemmed tendril climber commonly called as Shivlingi. Traditionally, this plant has been used in the folk medicine and possesses several activities such as gynaecological, anti-asthmatic, anti-convulsant, anti-venom, anti-inflammatory, androgenic and antioxidant 2-4 . The family Cucurbitaceae has been recognized as a rich source of bitter compounds, called cucurbitacins 5 . The cucurbitacins are highly unsaturated triterpenes containing many keto-, hydroxy-and acetoxy-groups. They are the major active components in traditional medicine, herbal remedies and Pharmacopoeia to treat various health issues such as hepatoprotective, anti-fertility, diabroticites cardiovascular and anti-inflammatory activities 6 . In a recent study, cucurbitacin I (CUI) has been identified as a strong inhibitor of the JAK2/STAT3 signaling pathway (a common oncogenic signaling pathway), which is constitutively activated in many types of cancer; hence, it is considered as a milestone in cancer therapy 7 . Similarly...

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Section: α-Amylase Inhibition Activity Diabetes Is One Of the Main Smentioning

confidence: 99%

Section: α-Amylase Inhibition Activity Diabetes Is One Of the Main Smentioning

confidence: 99%

Section: Antioxidant Activitiesmentioning

confidence: 99%

Section: Antioxidant Activitiesmentioning

confidence: 99%

Section: Antioxidant Activitiesmentioning

confidence: 99%

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Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Patel

Attar

Sakate

et al. 2020

Sci Rep

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Importance of Underground Storage Organs in Plants (for Their Survival and Perpetuation and for Human Welfare)

Murthy

Yadav

Paek

et al. 2023

Reference Series in Phytochemistry

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Importance of Underground Storage Organs in Plants (for Their Survival and Perpetuation and for Human Welfare)

Murthy,

Yadav,

Paek

et al. 2024

Reference Series in Phytochemistry

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Antioxidant, anti-diabetic, acetylcholinesterase inhibitory potential and estimation of alkaloids (lycorine and galanthamine) from Crinum species: An important source of anticancer and anti-Alzheimer drug

Cited by 77 publications

References 52 publications

Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Efficient extraction of cucurbitacins from Diplocyclos palmatus (L.) C. Jeffrey: Optimization using response surface methodology, extraction methods and study of some important bioactivities

Importance of Underground Storage Organs in Plants (for Their Survival and Perpetuation and for Human Welfare)

Importance of Underground Storage Organs in Plants (for Their Survival and Perpetuation and for Human Welfare)

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