Antioxidant, anti-inflammatory, analgesic and anti-proliferating activities of Grewia heterotricha Mast.

Abstract

Introduction and Aim: Plants are considered to be novel source of active compounds having pharmacological properties and help in the development of therapeutic agents. Hence, this study was undertaken to evaluate the antioxidant, anti-inflammatory, analgesic and anti-proliferating activity of aqueous and methanolic leaf extracts of Grewia heterotricha Mast.
 Materials and Methods: The aqueous and methanolic leaf extracts of the plant were assessed for their in-vitro antioxidant activity by DPPH radical scavenging activity, in-vivo anti-inflammatory activity by carrageenan induced rat paw edema method, in-vivo analgesic activity by acetic acid-induced writhing test and in-vitro anti- proliferating activity by MTT assay.
 Results: The methanolic extract had shown very significant DPPH radical scavenging activity with IC50 value 98.95?g/ml than aqueous extract and showed a significant reduction in the paw volume of rats at the concentration of 100 mg/kg body weight indicating potent anti-inflammatory activity compared with the reference standard Diclofenac sodium. Both the extracts showed significant analgesic effect (p<0.001) in acetic acid-induced pain models in a dose dependent manner. The methanolic extract showed higher analgesic activity compared to aqueous extract by inhibiting the pain indicated by a decrease in the number of writhes. In addition, both the extracts showed a decrease in MCF-7 cell viability at the concentration of 550µg/ml. Compared to the aqueous extract, MEGH has shown more cytotoxic effect on the cancer cell lines.
 Conclusion: The results suggest that both aqueous and methanolic extracts of Grewia heterotricha Mast. leaves possess potent antioxidant, analgesic, anti-inflammatory and anti-proliferating properties, which supports the use of the plant in traditional medicine. Further investigation is required to illuminate on its active compounds.
 
 Keywords: Analgesic; anti-inflammatory; DPPH; cytotoxic.

Preliminary phytochemical screening, physicochemical analysis and in-vitro antioxidant activity of selected Holigarna species- Endemic plant species of Western Ghats

“…The potential DPPH radical scavenging activity observed in this study may be due to the presence of these secondary metabolites in the extracts. These findings may mediate through any of the above signalling pathways (33). Our observation is in agreement with the studies of Izuegbuna et al, (34).…”

Synthesis and characterization of 2-thiophen flavonoid analogue for free radical scavenging antioxidant analysis

“…KBr disc method was employed to get IR spectra through FTIR spectrometer-8300(Shimadzu, Japan) and FTIR 4100. 1 H-NMR and 13 C-NMR spectra were recorded on High resolution Ft-MR Multinuclear SpectrometerBrukar) in deuterated chloroform with internal standard tetramethylsilane(TMS), working at 400MHz and 75MHz frequencies, respectively.…”

“…n recent years, there is an increase attention towards antioxidants usages in oxidative stress induced diseases, because of their protection over various cells, their organelles and even over metabolic pathways from the harmful oxidative radicals (1,2). Free radicals are electrically charged unpaired electrons.…”