Antioxidant, anti-inflammatory and healing potential of ethyl acetate fraction of Bauhinia ungulata L. (Fabaceae) on in vitro and in vivo wound model

Rodrigues, Rodrigo Rodrigues; Yaochite, Juliana Navarro Ueda; Sasahara, Greyce Luri; Albuquerque, Andressa Almeida; Fonseca, Said Gonçalves da Cruz; Araújo, Thiago Dias de Vasconcelos; Santiago, Gilvandete Maria Pinheiro; Sousa, Leôncio M. de; Carvalho, Jarbas Lima de; Alves, Ana Paula Negreiros Nunes; Carvalho, Paulo Germano de; Nagão-Dias, Aparecida Tiemi

doi:10.1007/s11033-020-05332-7

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…Importantly, this function of Pldo/ZSWIM8 is conserved from Drosophila to mammals. In a well-established wound healing cell migration assay in the human A549 cell line ( Piao et al, 2014 ; Han et al, 2016 ; Wang et al, 2017 ; de Oliveira Rodrigues et al, 2020 ; Kim et al, 2020 ; Liao & Peng, 2020 ; Zhao et al, 2020 ), we observed that pldo/zswim8 knockout cells migrate significantly faster than control cells, suggesting that these cells have an increase in branched actin polymerization, allowing a faster migration. Strikingly, this effect is rescued by providing the Drosophila Pldo/ZSWIM8 protein to these knockout human A549 cells, which would increase the competition for actin monomers, reducing the branched polymerization.…”

Section: Discussionmentioning

confidence: 84%

The conserved Pelado/ZSWIM8 protein regulates actin dynamics by promoting linear actin filament polymerization

et al. 2022

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Actin filament polymerization can be branched or linear, which depends on the associated regulatory proteins. Competition for actin monomers occurs between proteins that induce branched or linear actin polymerization. Cell specialization requires the regulation of actin filaments to allow the formation of cell type–specific structures, like cuticular hairs in Drosophila, formed by linear actin filaments. Here, we report the functional analysis of CG34401/pelado, a gene encoding a SWIM domain–containing protein, conserved throughout the animal kingdom, called ZSWIM8 in mammals. Mutant pelado epithelial cells display actin hair elongation defects. This phenotype is reversed by increasing actin monomer levels or by either pushing linear actin polymerization or reducing branched actin polymerization. Similarly, in hemocytes, Pelado is essential to induce filopodia, a linear actin-based structure. We further show that this function of Pelado/ZSWIM8 is conserved in human cells, where Pelado inhibits branched actin polymerization in a cell migration context. In summary, our data indicate that the function of Pelado/ZSWIM8 in regulating actin cytoskeletal dynamics is conserved, favoring linear actin polymerization at the expense of branched filaments.

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Section: Discussionmentioning

confidence: 84%

The conserved Pelado/ZSWIM8 protein regulates actin dynamics by promoting linear actin filament polymerization

et al. 2022

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“…Importantly, this function of Pldo/ZSWIM8 is conserved from Drosophila to mammals. In a well-established wound healing cell migration assay in the human A549 cell line (Han et al, 2016; Kim et al, 2020; Liao and Peng, 2020; de Oliveira Rodrigues et al, 2020; Piao et al, 2014; Wang et al, 2017; Zhao et al, 2020), we observed that pldo/zswim8 knockout cells migrate significantly faster than control cells, suggesting that these cells have an increase in branched actin polymerization, allowing a faster migration. Strikingly, this effect is rescued by providing Drosophila Pldo/ZSWIM8 protein to these knockout human A549 cells.…”

Section: Discussionmentioning

confidence: 84%

The conserved Pelado/ZSWIM8 protein regulates actin dynamics by promoting linear actin filament polymerization

Pelayo

Olguı́n

Mlodzik

et al. 2022

Preprint

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Actin filament polymerization can be branched or linear, which depends on the associated regulatory proteins. Competition for Actin monomers occurs between proteins that induce branched or linear actin polymerization. Cell specialization requires the regulation of actin filaments to allow the formation of cell-type specific structures, like cuticular hairs in Drosophila, formed by linear actin filaments. Here, we report the functional analysis of CG34401/pelado, a gene encoding a SWIM domain containing protein, conserved throughout the animal kingdom. Mutant pelado epithelial cells display actin hair elongation defects. This phenotype is reversed by increasing Actin monomer levels or by either pushing linear actin polymerization or reducing branched actin polymerization. The same behavior occurs in hemocytes, where Pelado is essential to induce filopodia, a linear actin-based structure. We further show that this function of Pelado, ZSWIM8 in mammals, is conserved in human cells, where Pelado inhibits branched actin polymerization in a cell migration context. In summary, our data indicate that the function of Pelado/ZSWIM8 in regulating actin cytoskeletal dynamics is conserved, favoring linear actin polymerization at the expense of branched filaments.

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“…In this study, we intended to prospect the effect of menthol in skin wound healing. Skin wounds were developed in rats and treated with menthol-based creams for 3, 7, or 14 days, according to previously described stages of wound healing: inflammatory, proliferative, and tissue remodeling phase [9]. This is the first study evaluating the in vivo wound healing potential of menthol, and we have demonstrated that, topically applied, menthol at the concentration of 0.5% accelerated the wound closure in 3, 7, and 14 days of treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Such enzymes and protein scavengers free radicals and prevent oxidative damage. The effectiveness of the wound healing process is directly related to the antioxidant ability of the therapeutic agent, since removing free radicals significantly accelerates the healing process, reducing tissue damage and wound infection [9,37]. The activity of MPO was also measured as an indicator of pro-oxidant and pro-inflammatory activity.…”

Section: Mpo and Antioxidant Activitiesmentioning

confidence: 99%

“…In modern medical sciences, searching for drugs that accelerate wound healing with minimal pain, discomfort, and scarring is a promising area of research [6]. Several studies have successfully tested the effects of medicinal plants in skin wound healing, in which the extracts or their isolated compounds accelerated skin wound closure, inhibited local inflammation, and increased cellular antioxidant defense [8,9]. Plant-based therapy is known to accelerate wound healing as well as maintaining the aesthetics in a natural way [10]; this is the reason why more than 70% of wound healing pharmaceutical products are derived from plants [11].…”

Section: Introductionmentioning

confidence: 99%

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The Use of Menthol in Skin Wound Healing—Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization

et al. 2021

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Wound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-α and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization.

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Antioxidant, anti-inflammatory and healing potential of ethyl acetate fraction of Bauhinia ungulata L. (Fabaceae) on in vitro and in vivo wound model

Cited by 11 publications

References 33 publications

The conserved Pelado/ZSWIM8 protein regulates actin dynamics by promoting linear actin filament polymerization

The conserved Pelado/ZSWIM8 protein regulates actin dynamics by promoting linear actin filament polymerization

The conserved Pelado/ZSWIM8 protein regulates actin dynamics by promoting linear actin filament polymerization

The Use of Menthol in Skin Wound Healing—Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization

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