Antioxidant–Anti-Inflammatory Evaluation of a Polyherbal Formula

Abstract

Most disease—both acute and chronic—results from inflammation, and reactive oxygen species (ROS) are considered some of the strongest stimuli of inflammation. Many studies reported the traditional use of herbal species for treating inflammation, especially when ROS are involved. The present study aims to demonstrate the antioxidant–anti-inflammatory effects of a patented preparation based on Populus nigra and Rosmarinus officinalis extracts and to highlight its applicative potential; the formula was characterized by HPTLC and HPLC and in-vitro studies were conducted on TNF-α-stimulated HUVECs. The antioxidant activity of the formula was determined by DPPH assay and the phosphomolybdenum method; to assess in-vivo anti-inflammatory activity, a rat paw edema model was used; the formula contains high amounts of polyphenols. It exhibited scavenging activity of 50–85% at 1–10 mg/mL, it inhibited nitrite production and ICAM-1 expression in TNF-α-stimulated endothelial cell cultures dose-dependently, at a maximum of 58.7% at the maximum dose administered and exerted an obvious anti-inflammatory effect in vivo, settling early and decreasing at 180 min; a new herbal bioactive product was presented with promising therapeutic potential that can be an adjunct to conventional therapies for diseases based on oxidative stress and inflammation.

Novel Antidiabetic Polyherbal Formulation for Synergistic Therapeutic Effects in Streptozotocin (STZ)-Induced Diabetic Rats

“…The findings of present study are similar with the findings of others that demonstrated the substantial antihyperglycemic properties of polyherbal formulation in STZ induced diabetic rats. [7][8][9][10][11][12][13][14][15][16][17][18][19][20][21] Our findings are similar with the findings of other authors. It has been demonstrated that our discoveries can decrease blood sugar levels in diabetic animals.…”

“…Compared to individual natural products, each benefit has contributed to the increased popularity of polyherbal formulation on the trade. 7,8 Azadirachta indica, Tinospora cordifolia and Oscimum sanctum are medicinal plants used for centuries to heal hyperglycemia. Thus, it was intended to explore the anti-diabetic properties of a polyherbal formulation containing different ratios of A. indica leaves, T. cordifolia stem and O. sanctum leaves.…”

Phytochemical Constituents and Biological Activity of Wild and Cultivated Rosmarinus officinalis Hydroalcoholic Extracts

“…For instance, it has been demonstrated that rosemary extracts are effective in the treatment of orchitis by exerting an antioxidant effect on murine sperm [ 62 ]. Likewise, a polyherbal formula based on Populus nigra L. and R. officinalis extracts demonstrated a therapeutic potential for diseases based on oxidative stress and inflammation [ 63 ]. Differently from data on extracts, many reports are available regarding the anti-inflammatory effect of carnosic acid and carnosol using different in vitro and in vivo models of inflammation [ 64 ].…”

In Vitro and In Vivo Anti-Inflammatory Potential of Cannabichromene Isolated from Hemp

“…Infectious and noninfectious substances and cellular damage activate inflammatory cells, usually through the nuclear factor kappa B (NF-kB) and mitogenactivated protein kinase (MAPK) pathways [6]. In addition, the carrageenan-induced mouse model is one of the animal experimental models that can be used for evaluating the efficacy of anti-inflammatory agents, and it has been determined that an increase or decrease in inflammation-related cytokines can be confirmed using this experimental model [11,12].…”

“…To confirm the reduction in inflammatory factors following oral administration of CBC, we inoculated 0.5% carrageenan into the paws of mice [11,12] (Figure 7A). The edematous tissues were removed, and proteins were extracted to analyze the inflammatory factors using enzyme-linked immunosorbent assay (ELISA).…”