Antioxidant, antibacterial and  in vivo  dermal wound healing effects of  Opuntia  flower extracts

Ammar, Imen Ben; Bardaa, Sana; Mzid, Massara; Sahnoun, Zouheir; Rebaii, Tarak; Attia, Hamadi; Ennouri, Monia

doi:10.1016/j.ijbiomac.2015.08.039

Cited by 68 publications

(43 citation statements)

References 37 publications

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“…CMLA possesses antioxidant activity as it increased the activities of antioxidant enzymes in tissue homogenates. The reactive intermediates from oxidation could delay wound healing15. Moreover, wound healing may be accelerated by the anti-inflammatory properties of CMLA.…”

Section: Discussionmentioning

confidence: 99%

“…1) was dissolved as described by Shetty et al . with some alterations using the vehicle (gum acacia) in normal saline15.…”

Section: Methodology and Materialsmentioning

confidence: 99%

“…The tracing paper was placed on a sheet of graph paper (2 mm 2 ) and the number of squares within the wound area was counted19. The percentage of wound contraction was measured, using the initial area and the areas determined on days 1, 5, 10 and 15 using the following formula15:…”

Section: Methodology and Materialsmentioning

confidence: 99%

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In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

El-Ferjani

Ahmad

Dhiyaaldeen

et al. 2016

Sci Rep

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Co (II) complex (CMLA) was investigated to evaluate the rate of wound healing in rats. Animals were placed into four groups: gum acacia, Intrasite gel, 10 and 20 mg/ml of CMLA. Wounds were made on the dorsal neck area, then treated with Intrasite gel or CMLA; both of these treatments led to faster healing than with gum acacia. Histology of the wounds dressed with CMLA or Intrasite gel displayed a smaller scar width, required less time to heal and showed more collagen staining and fewer inflammatory cells in comparison to wounds dressed with the vehicle. Immunohistochemistry for Hsp70 and TGF-β showed greater staining intensity in the treated groups compared to the vehicle group. Bax staining was less intense in treated groups compared to the vehicle group, suggesting that CMLA and Intrasite gel provoked apoptosis, responsible for the development of granulation tissue into a scar. CD31 protein analysis showed that the treated groups enhanced angiogenesis and increased vascularization compared to the control group. Furthermore, a significant increase in the levels of GPx and SOD and a decrease in MDA were also observed in the treated groups. This results suggest that CMLA is a potentially promising agent for the wounds treatment.

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Section: Discussionmentioning

confidence: 99%

“…1) was dissolved as described by Shetty et al . with some alterations using the vehicle (gum acacia) in normal saline15.…”

Section: Methodology and Materialsmentioning

confidence: 99%

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In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

El-Ferjani

Ahmad

Dhiyaaldeen

et al. 2016

Sci Rep

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“…Beyond these attractive characteristics, several edible flowers are also rich in different nutrients and bioactive compounds such as proteins, carbohydrates, sugars, organic acids, terpenoids, carotenoids, flavonoids and vitamins (Mlcek & Rop, 2011). Due to the presence of bioactive compounds, especially flavonoids, edible flowers are also described as having different bioactivities such as antioxidant (Kumar, Bhandari, Singh, & Bari, 2009), antihypertensive (Xie & Zhang, 2012), antibacterial (Ammar et al, 2015), or antitumor (Sagdic et al, 2013), among others.…”

Section: Introductionmentioning

confidence: 99%

The influence of electron beam radiation in the nutritional value, chemical composition and bioactivities of edible flowers of Bauhinia variegata L. var. candida alba Buch.-Ham from Brazil

et al. 2018

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A B S T R A C TAs edible flowers are highly perishable, irradiation technology can be applied to increase their shelf life, as also for phytosanitary purposes. Herein, flowers of Bauhinia variegata L. var. candida alba Buch.-Ham were submitted to electron beam irradiation at the doses of 0.5, 0.8 and 1 kGy, to study the effects in the nutritional and chemical profiles, and also in antioxidant, cytotoxic and anti-inflammatory activities. The petals of white flowers revealed interesting bioactive properties being kaempferol derivatives the most abundant compounds, especially kaempferol-3-O-rutinoside. The applied irradiation doses did not highly affect the nutritional profile. No changes were produced in cytotoxicity, but the anti-inflammatory activity slightly decreased. However, the antioxidant activity was increased, especially in the dose of 0.5 kGy, in agreement with the higher content in phenolic compounds found at this dose.

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“…Estudos realizados com o gênero Opuntia comprovam a atividade cicatrizante (Park & Chun, 2001;Galati et al, 2003;Trombetta et al, 2006;Ammar et al, 2015), antioxidante (Butera et al, 2002;Semedo, 2012;Madrigal-Santillán et al, 2013), antinflamatória (Arauza, 2009;Galati et al, 2003) e antitumoral (Zou et al, 2005;Hahm et al, 2010;Poejo, 2011).…”

Section: Introductionunclassified

Avaliação da atividade antibacteriana, citotóxica e antioxidante da espécie vegetal Opuntia cochenillifera (L.) Mill

Silva

Araújo

Bastos

et al. 2016

Rev. bras. plantas med.

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RESUMO:O presente estudo teve como objetivo avaliar a atividade antibacteriana, antioxidante e citotóxica da espécie Opuntia cochenillifera (L.) Mill. Foi realizada a prospecção fitoquímica e espectroscopia de absorção de infravermelho (IV) dos extratos etanólicos brutos e frações dos cladódios grande e pequeno. A atividade antioxidante foi avaliada pelo método da capacidade sequestradora de radicais livres utilizando o radical sintético 2,2-difenil-1-picrilhidrazila (DPPH). A atividade citotóxica foi obtida através do método colorimétrico do Metiltetrazolium (MTT). Já a atividade antibacteriana foi avaliada pelo método de microdiluição em caldo para determinar a concentração inibitória mínima (CIM) frente às estirpes bacterianas Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa e Escherichia coli. A prospecção fitoquímica revelou principalmente a presença de fenóis, esteroides livres, alcaloides, alcanos, além de outras classes químicas. O IV apresentou grupos funcionais como alcanos, carbonilas, grupos de metila, duplas ligações de carbono, grupamentos alquilamina, entre outros. Sobre a citotoxicidade na concentração de 100 μg/mL, os dois extratos brutos, todas as frações do cladódio grande e as frações de clorofórmio e metanol do cladódio pequeno não apresentaram toxicidade. Os extratos brutos e frações do cladódio grande e pequeno, não demonstraram atividade antibacteriana e nem antioxidante. Esses resultados podem fornecer suporte para pesquisas futuras, visando outras atividades biológicas da presente espécie vegetal. Palavras-chave:Opuntia cochenillifera (L.) Mill. Composição química. Atividade antibacteriana. Atividade citotóxica. Antioxidante.ABSTRACT: Evaluation of antibacterial, cytotoxic, and antioxidant activity of the plant species Opuntia cochenillifera (L.) Mill. The purpose of this study was to evaluate the antibacterial, antioxidant, and cytotoxic activity of Opuntia cochenillifera (L.) Mill. A phytochemical screening and infrared (IR) absorption spectroscopy were performed in the crude ethanolic extracts and fractions of large and small cladodes. The antioxidant activity was evaluated through the qualitative method of free-radical scavenging capacity using the synthetic radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The cytotoxic activity was obtained by the cell viability assay using methyl thiazolyl tetrazolium (MTT). Antibacterial activity was evaluated by broth microdilution method to determine the minimum inhibitory concentration (MIC) against the bacterial strains Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli. The phytochemical screening mainly revealed the presence of phenols, flavonoids, free steroids, alkaloids, alkanes, and other chemical classes. The IR spectroscopy presented functional groups such as alkanes, carbonyls, methyl groups, carbon double bonds, and alkylamino groups, among others. Regarding cytotoxicity in the concentration of 100 μg/mL, neither the crude extracts, the fractions of the large cladode...

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Antioxidant, antibacterial and in vivo dermal wound healing effects of Opuntia flower extracts

Cited by 68 publications

References 37 publications

In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats

The influence of electron beam radiation in the nutritional value, chemical composition and bioactivities of edible flowers of Bauhinia variegata L. var. candida alba Buch.-Ham from Brazil

Avaliação da atividade antibacteriana, citotóxica e antioxidante da espécie vegetal Opuntia cochenillifera (L.) Mill

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