Antioxidant, Anticancer, Antibacterial, Antibiofilm Properties and Gas Chromatography and Mass Spectrometry Analysis of Manuka Honey: A Nature’s Bioactive Honey

Abstract

Honey has a history of medical use and is known as bio-alternative therapy. This research assessed the phytochemical and biological activity of the medical grade manuka honey (MH). Gas chromatography–mass spectrometry (GC–MS) was chosen to investigate bioactive compounds of the MH. The DPPH and ABTS free radical scavenging and beta-carotene antioxidant activities as well as the antibacterial and antibiofilm effects against S. aureus, B. subtilis, E. coli and P. aeruginosa were all determined. Furthermore, to gauge anticancer properties of MH, a MTT assay was opted towards three cell lines, including HCT-116 (colon), A549 (lung) and MCF-7 (breast) cancer cells. The GC–MS analysis of the tested MH revealed the identification of various chemical constituents belonging to the fatty acids, phenols, and esters. The MH was found to have higher reducing power activity (DPPH IC50: 7.36; ABTS IC50: 4.49 mg/mL) than the beta-carotene bleaching power (IC50: 37.51 mg/mL). Similarly, the MH was noted to be more active against the planktonic and biofilm of Gram-positive bacteria. Furthermore, a dose-dependent anticancer potential was observed, although a significant anticancer potential was pointed out against the MCF-7 and A549 cell conforming to the IC50 values of 9.05 and 9.37 mg/mL, respectively. This study’s results have highlighted the MH’s chemical composition with significant bioactivities.

Phytochemical Characterization and Efficacy of Artemisia judaica Extract Loaded Chitosan Nanoparticles as Inhibitors of Cancer Proliferation and Microbial Growth

“…Plates were incubated at 37 °C, 5% CO 2 , overnight to investigate any possible cytopathic effects of treated cells. A solution of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) was prepared using phosphate buffered saline (PBS) as a solvent (5 mg/mL) [ 24 ]; then 20 µL of the prepared solution was added into each well, followed by well-mixing of prepared solution with the used medium through a shaking table at 150 rpm for 5 min [ 25 ]. The well plates were incubated at 37 °C for 4 h under 5% CO 2 to initiate/enhance the metabolizing of MTT.…”

Evaluation of the effects of a honey‑based gel on blood redox biomarkers and the physiological profile of healthy adults: A pilot study

“…Regarding the results, the honey-based gel used in the present study, exhibited the lowest IC 50 value, determined using an ABTS assay, compared with both the 21 different Greek honey types and Manuka ( 66 ). Bazaid et al investigated the scavenging activity of Manuka honey using ABTS RSC assay among other assays ( 67 ). Concerning the results of the present study, the honey-based gel exhibited a lower IC 50 value (0.88 mg/ml) than the assessed Manuka honey (4.49 mg/ml) ( 67 ).…”

“…Bazaid et al investigated the scavenging activity of Manuka honey using ABTS RSC assay among other assays ( 67 ). Concerning the results of the present study, the honey-based gel exhibited a lower IC 50 value (0.88 mg/ml) than the assessed Manuka honey (4.49 mg/ml) ( 67 ). The present study is part of a larger project investigating Greek honey bioactivity.…”

Juglans regia Linn.: A Natural Repository of Vital Phytochemical and Pharmacological Compounds

“…Consuming nuts on a regular basis has been linked to a lower risk of both fatal and non-fatal myocardial infarction. According to epidemiological studies, compared to people who never consumed nuts, those who consumed nuts five or more times per week had a 50% lower risk of coronary heart disease [ 105 , 106 ]. Green walnut hull extract suppressed protein secretion and thrombin-induced platelet aggregation by 50% in an in vitro investigation, with no deleterious effects on platelets at a dosage of 50 mg/mL.…”