Antioxidant, Antidiabetic, and Antiobesity Properties, TC7-Cell Cytotoxicity and Uptake of Achyrocline satureioides (Marcela) Conventional and High Pressure-Assisted Extracts

Abstract

The growing incidence of non-communicable diseases makes the search for natural sources of bioactive compounds a priority for such disease prevention/control. Achyrocline satureioides (‘marcela’), a plant rich in polyphenols and native to Brazil, Uruguay, Paraguay, and Argentina, could be used for this purpose. Data on its antidiabetic/antiobesity properties and cellular uptake of bioactive compounds are lacking. The potentiality of non-thermal technologies such as high-hydrostatic pressure (HP) to enhance polyphenol extraction retains attention. Thus, in the present study aqueous and ethanolic marcela extracts with/without assisted-HP processing were chemically characterized and assessed for their in vitro antioxidant capacity, antidiabetic and antiobesity activities, as well as cellular cytotoxicity and uptake on intestinal cell monolayers (TC7-cells, a clone of Caco-2 cells). Aqueous and ethanolic conventional extracts presented different polyphenolic profiles characterized mainly by phenolic acids or flavonoids, respectively, as stated by reverse phase-high-performance liquid chromatography (RP-HPLC) analyses. In general, ethanolic extracts presented the strongest bioactive properties and HP had none or a negative effect on in vitro bioactivities comparing to conventional extracts. TC7-cell viability and cellular uptake demonstrated in conventional and HP-assisted extracts, highlighted the biological effects of marcela bioactive compounds on TC7-cell monolayers. TC7-cell studies showed no HP-induced cytotoxicity. In sum, marcela extracts have great potential as functional ingredients for the prevention/treatment of chronic diseases such as diabetes.

Effect of Hydrogel Containing Achyrocline satureioides (Asteraceae) Extract–Loaded Nanoemulsions on Wound Healing Activity

“…Thes indicate that these wounds had less lipid damage and, consequently, less ox damage. Researchers have shown the antioxidant activity of extracts of A. saturei different experimental conditions, which has mainly been related to the prese phenolic compounds [18,[28][29][30]. Pereira et al [12] compared Achyrocline satureioides and Achyrocline alata on skin wound healing in mice and found that A. alata was more effective because it induced earlier wound closure than A. satureioides, although the latter improved collagen renewal compared with A. alata [12].…”

“…These data indicate that these wounds had less lipid damage and, consequently, less oxidative damage. Researchers have shown the antioxidant activity of extracts of A. satureioides in different experimental conditions, which has mainly been related to the presence of phenolic compounds [18,[28][29][30]. MPO is an important pro-oxidant enzyme with antimicrobial action.…”

Potential of Onion Byproducts as a Sustainable Source of Dietary Fiber and Antioxidant Compounds for Its Application as a Functional Ingredient

“…The potential antidiabetic effect of the samples (OS and OP) was evaluated, determining the capacity to inhibit α-glucosidase enzyme [11]. This inhibition was determined by the fluorescence (λexcitation = 360 nm, λemission = 460 nm) generated from the hydrolysis of the enzyme substrate (4-MUF-α-D-glucopyranoside) by α-glucosidase over a 30 min duration at 37 • C using a Varioskan TM Lux (SkanIt RE 5.0 software, Thermo Scientific, Waltham, MA, USA) fluorimeter microplate reader.…”

Achyrophenols A–F: Polycyclic Polyphenol Lactone Skeletons and a Nor-Ursane-Type Triterpenoid from Achyrocline Satureioides

“…As a common folk herb, it is mainly used for the treatment of gastrointestinal diseases and bacterial and viral infections as it has anti-inflammatory and analgesic effects. , It has been reported that its methanolic extracts had a certain anti-hepatoma effect against a human hepatocellular carcinoma cell line, Hep G2 . The cytotoxic activity against A375-derived human melanoma cell lines and antioxidant and anti-inflammatory activities were successively reported in recent years, − which suggested strong medicinal potentials. Through the further research of the chemical constituents of A. satureioides , it was found that there were many bioactive chemical constituents, including flavonoids and phenylpropanoids. − We have been devoted to the localization of A. satureioides for several years and were surprised to find two unprecedented derivatives, 1 and 2 , of 2-methyl-1-(2,3,4,6-tetrahydroxyphenyl)­propan-1-one and ( S )-2-methyl-1-(2,3,4,6-tetrahydroxyphenyl)­butan-1-one, respectively.…”