Antioxidant, antimicrobial, and antiproliferative activity-based comparative study of peel and flesh polyphenols from Actinidia chinensis

Abstract

Background Kiwifruit ( Actinidia chinensis ) peel has been always considered as useless because of the harsh taste. To promote the full utilization of kiwifruit resources it is essential to explore the nutritional benefits of kiwifruit peel. Objective Our studies explored the difference in polyphenolic composition and biological activity including antioxidant, antimicrobial, and antiproliferative activity of the flesh and peel of kiwifruit. Design Antioxidant activity of the extracted polyphenols of the peel and flesh of A. chinensis was checked by 2,2-diphenyl-1-picrylhydrazyl, 2,2’-azino-bis3-ethylbenzothiazoline-6-sulphonic acid (ABTS), hydroxyl ion reduction, and ion chelating ability. Antibacterial activity against Escherichia coli , Listeria monocytogenes , and Staphylococcus aureus and antiproliferative activity against HepG2 was tested in a dose- and time-dependent manner. Liquid chromatography/mass spectrometry (LC/MS) chromatogram of the peel and flesh further differentiated the phenolic acid profile. Results The pericarp of kiwifruit was found to be more abundant in polyphenols and flavonoids than the flesh, with contents of 12.8 mg/g and 2.7 mg/g, respectively. LC/MS analysis revealed that the catachin, quercetin and epigallocatechin content (the main polyphenols in kiwifruit) in the peel was significantly higher than in the flesh ( P < 0.05). The antioxidant and antibacterial activity of the peel was significantly higher when compared to the flesh. Moreover, the proliferation of HepG2 cells was time- and dose-dependently inhibited by kiwifruit polyphenols, with IC 50 values of 170 μg/mL and 291 μg/mL for peel and flesh polyphenols after 72 h of treatment time, respectively. Conclusion Kiwifruit peel, with higher content of phenolics and flavonoids, exerts more potent antioxidant, antibacterial, and anticancer activity than the flesh. Our study provides scientific evidence for the development of kiwifruit, especially peel-based, novel natural products with excellent bioactivity.

Comparative assessment of phytochemical profiles and antioxidant and antiproliferative activities of kiwifruit ( Actinidia deliciosa ) cultivars

“…Catechin as one main content in our study is consistent with previous report. Alim et al () found that the main compounds also included quercetin and epigallocatechin. Several factors can influence the phytochemicals contents such as cultivars, the degree of fruit ripening, environmental conditions, and the extraction methods (Zhu et al, ).…”

“…Catechin as one main content in our study is consistent with previous report. Alim et al (2019) found that the main compounds also included quercetin and epigallocatechin.…”

Antioxidant Activity of Kiwi Fruit (Actindia Chinensis)

“…Various studies have examined the antioxidant activity of both peel and lesh of kiwi fruit in different in-vitro assay systems (Zuo et al, 2012). In most cases, the kiwi fruit extract showed moderate antioxidant activity, but the peel extract seemed to have more polyphenols and lavonoids which in turn have high antioxidant activity than the lesh of the fruit (Alim et al, 2019). The study illustrated that Actinidia chinensis peel was found to have a greater number of speci ic polyphenols and lavonoids, which are responsible for the higher antioxidant, antimicrobial, and antiproliferative activity when compared to the lesh of the fruit.…”

“…Kiwi fruit is distinct for its soft texture and its unique lavours. Comparative studies on kiwi fruit peel and its lesh have been conducted (Alim et al, 2019). The peel of kiwifruit was identi ied to have a high content of phenolics and lavonoids; and exerts more potent antioxidant, antibacterial and anticancer activity than its lesh.…”

Efek Buah Kiwi (Actinidia Deliciosa) Sebagai Pengobatan Luka Bakar Derajat II

“…Berdasarkan peranan yang cukup besar dari buah kiwi yang diantaranya membantu proses debridement dan pelepasan jaringan parut tanpa merusak proses penyembuhan, perbaikan angiogenesis, epitelisasi dan granulasi, serta efeknya terhadap penurunan ukuran luka menunjukkan bahwa buah kiwi dapat menjadi pilihan pengobatan yang menjanjikan bagi upaya terapi luka bakar (Pisoschi et al, 2015). Penelitian lain yang dilakukan oleh Alim et al (2019) aktivitas antibakterial yang lebih signifikan ketika menggunakan balut buah kiwi dibandingkan dengan pemberian sulfadiazine silver pada hewan yang dicobakan. Mohajeri dkk mengindikasikan bahwa pembalutan dengan buah kiwi mempercepat penyembuhan luka pada luka bakar dan mereka menyatakan efek ini berasal dari antibakterial yang dramatis.…”