Antioxidant, Antimicrobial and Cytotoxic Activities of <i>Desmodium Motorium</i> Merr.

Abstract

The n-hexane, carbon tetrachloride, dichloromethane soluble fractions of methanol extract of the whole plant of Desmodium motorium were subjected to antioxidant activity by indicated method, inhibition of microbial growth by disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The methanol i.e., crude extract showed highest antioxidant activity having IC 50 9.5 μg/ml as compared to 9.0 μg/ml for standard agent BHT. The n-hexane, carbon tetrachloride and dichloromethane soluble fraction showed moderate inhibitory activity to microbial growth, while all the extractive exhibited strong cytotoxic property with the carbon tetrachloride soluble materials revealed the strongest cytotoxicity with LC 50 1.54 μg/ml.

Studies on desmodium velutinum (Willd.) DC. leaf extract's polyphenol profile, in-vitro antioxidant capacity, and anti-proliferative activity of A549 cell lines

“…The more effective the plant material is at scavenging DPPH and hence, the lower the IC50 value, the greater the antioxidant activity of the plant material(Olugbami et al, 2014). Conversely, lower IC50 values in DPPH assay have been reported byChowdhury et al, (2013) in methanol extract and fractions of D. motorium. Comparing the antioxidant potency of the crude extract, and its fractions, it was observed that ethyl-acetate fraction (E.F) of D. vulutinum demonstrated a better antioxidant activity than the crude extract, M.E and the n-butanol fraction, B.F in DPPH, CUPRAC and reducing power assays.…”

Evaluation of Antiradical Activity Grape Seeds and Olive (<i>Olea europaea</i>) Pits Extracts

“…Totally, plants and animals protect themselves by a complex system from multiple antioxidants, such as glutathione, tocopherols, carotenoids, ascorbic acid, flavonoids and tannins, and vitamin E along with some enzymes like catalase, superoxide dismutase and various peroxidases [2,5]. Therefore, pharmacognosy is an important therapeutic method for various diseases, as herbalism and folk medicine, both ancient and modern, were already existed in this fold [10]. On the other hand, there are some synthetic antioxidants such as butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), tertiary butylated hydroquinone and gallic acid esters which are used in food industries and other materials that prompt negative health effects [2,5,6].…”

“…In recent years, many epidemiological data have shown that consumption of vegetables and fruit as natural antioxidants may delay protect the human body from free radicals, prevent oxidative stress and or even prevent the onset of cardiovascular disorders, certain types of cancer, and other chronic dysfunctions, amoebic dysentery, chest pain, constipation, diarrhea, leucoderma and strangury, cough, fever, asthma, neurodegenerative diseases, aging, inflammation, atherosclerosis, diabetes [1][2][3][7][8][9], because there are phenolics that exist in all of plants, flavonoids, anthocyanins, tannins and carotenoids which have the ability to protect the body from oxidative stress caused by free radical [1,2,10]. Totally, plants and animals protect themselves by a complex system from multiple antioxidants, such as glutathione, tocopherols, carotenoids, ascorbic acid, flavonoids and tannins, and vitamin E along with some enzymes like catalase, superoxide dismutase and various peroxidases [2,5].…”