Antioxidant, Antimicrobial, and Protein Kinase Inhibition Profiling of C. ambrosioides Seed Extracts along with RP-HPLC

Abstract

The validation of underexplored traditional plant remedies represents a reservoir of novel leads for drug discovery. In line with this, in vitro total phenolics and flavonoids content, multimode antioxidants, antimicrobial, cytotoxicity, and protein kinase inhibition assays were conducted on C. ambrosioides seed extracts in addition to RP-HPLC. Methanol extract exhibited highest total phenolics ( 64.6 ± 0.6 μ g gallic acid equivalent/mg) and flavonoids ( 50.9 ± 0.5 μ g quercetin equivalent/mg) content. RP-HPLC quantified rutin (1.98 μg/mg) in methanol extract whereas quercetin (0.322 μg/mg) and kaempferol (2.86 μg/mg) in methanol-distilled water extract. Methanol extract exhibited highest ascorbic acid equivalent (AAE) free radical (DPPH) scavenging (IC50 of 110.7 ± 5 μ g / ml ), total antioxidant capacity ( 110.6 ± 2.2 μ g AAE/mg), and total reducing power ( 94.30 ± 0.46 μ g AAE/mg). Highest antibacterial activity against K. pneumonia ( 14 ± 1.61 mm ZOI) and antifungal activity against F. solani ( 17 ± 1.38 mm ZOI) were shown by n-hexane and chloroform extracts, respectively. Ethyl acetate extract exhibited highest brine shrimps cytotoxicity (LC50 of 125 μg/ml). A noteworthy protein kinase inhibitory potential was shown by ethanol extract with a 20 ± 1.27 mm bald zone. Therapeutic potential of medicinal plants can be completely explored by using multiple solvent system. This study makes C. ambrosioides, a resourceful prospect for the bioactivity-guided isolation of lead compounds.

Antioxidant, antidiabetic, neuroprotective, and phytochemical evaluation of Chenopodium ambrosioides ethanol extract

“…[36] In a research, Munir et al [37] determined the quantitative phytochemicals of Epimedium grandiflorum and concluded that total flavonoid (241.8 ± 11.18 mg CE/g dry plants material) and phenolic contents (1010.72 ± 12.49 mg GAE/g dry plants material) were present in E. grandiflorum. In another study, [17] C. ambrosioides methanol extracts from seeds revealed the highest phenolic (64.6±0.6µg gallic acid equivalent/mg) and flavonoid (50.9±0.5µg quercetin equivalent/mg) levels. This extract displayed potent antioxidant properties, showing strong free radical scavenging (IC50 of 110.7±5µg/ml), total antioxidant capacity (110.6±2.2µg AAE/mg), and total reducing power (94.30±0.46µg AAE/mg).…”

“…[14] Chenopodium ambrosioides (C. ambrosioides) has an abundance of phytochemicals with significant pharmacological properties including antioxidant, antiinflammatory, anthelmintic, antileishmanial, antitumoral, respiratory problems, tuberculosis, enzyme inhibition, and aiding wound healing and cancer prevention. [15][16][17] The objective of the current study is to explore the potential of C. ambrosioides extract (CAE) as a natural remedy for diabetes and neurodegenerative disorders. We investigated CAE's antioxidant potential and ability to inhibit enzymes linked to the above conditions.…”