Antioxidant, Antimicrobial, Cytotoxic, Larvicidal and Anthelmintic Activities and Phenolic Contents of &amp;lt;i&amp;gt;Cyclamen alpinum&amp;lt;/i&amp;gt;

Turan, Metin; Mammadov, Ramazan

doi:10.4236/pp.2018.94008

Cited by 23 publications

(18 citation statements)

References 25 publications

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“…Different C. species exhibited antimicrobial properties in varying degrees, such as C. hederifolium growing in Syria [31,35,36] . However, we may not compare our results to it, since extracts used in that research were made by different solvents that have no application in traditional medicine (methanol, petroleum ether, ethyl acetate and chloroform).…”

Section: Antioxidant Activitymentioning

confidence: 99%

Chemical and pharmacological characterization of aqueous and ethanolic extracts of Cyclamen hederifolium Ait. (Primulaceae) tuber

et al. 2021

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Background / Aim. Cyclamen hederifolium Ait. belongs to the family Primulaceae, which comprehends 23 species of cyclamen, naturally distributed in the Central and Southern Europe, Western Asia and some parts of North Africa. This plant is considered highly poisonous and not suitable for human use. However, tuber extracts have been used in traditional medicine and homeopathy. We have evaluated C. hederifolium growing naturally in Serbia for its metal content and biological activities (antioxidant, antibacterial, antifungal and cytotoxic activity). Methods. Content of metals was determined by atomic absorption spectrophotometric method. We used several different assays for assessment of antioxidant activity of both aqueous and ethanol extracts of C. hederifolium: DPPH radical scavenging assay, ABTS radical scavenging assay, Ferric-reducing antioxidant power (FRAP) assay, Total reducing power assay (TRP) and Cupric reducing antioxidant capacity (CUPRAC) assay. The disk diffusion assay was used to investigate sensitivity of laboratory bacterial and fungal control strains against investigated extracts. Aqueous and ethanol extracts of C. hederifolium were examined on 4 different tumoral cell lines by in vitro MTT bioassay. Results. The presence of Mn, Ca, Mg, Fe, Zn, K, Cu was confirmed in aqueous and ethanol extract, as well as in whole tubers and soil, while Cr, Ni, Pb and Cd were not detected. Both aqueous and ethanol extract of C. hederifolium tubers exhibited antioxidant activity, that positively correlates to content of phenols and flavonoids in it. Aqueous extract was slightly superior in these terms than ethanol extract. None of the tested extracts showed antimicrobial activity. Both investigated extracts showed cytotoxicity against four cancer cell lines 4T1, HCT116, CT26, LLC1, in the concentration range 15.625-2000 μg/ml. Ethanol extract showed stronger cytotoxicity than aqueous extract. Conclusion. Seven metals have been identified in the C.hederifolium tubers, extracts and soil. Both extracts exhibited antioxidant and cytotoxic activity.

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Section: Antioxidant Activitymentioning

confidence: 99%

Chemical and pharmacological characterization of aqueous and ethanolic extracts of Cyclamen hederifolium Ait. (Primulaceae) tuber

et al. 2021

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“…On the basis of their chemical composition, they are classified as flavonoids and non-flavonoids [ 90 ]. Flavonoids can be classified into several sub-classes such as flavones, flavonols, flavanones, and isoflavonoids [ 91 ], whereas non-flavonoids generally comprise phenolic and ellagic acids, chalcones, anthraquinones, stilbenes, and ellagitannins [ 92 ].…”

Section: Resultsmentioning

confidence: 99%

Anthocyanins and Carotenoids Characterization in Flowers and Leaves of Cyclamen Genotypes Linked with Bioactivities Using Multivariate Analysis Techniques

et al. 2022

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The present study was carried out to evaluate and compare in vitro antioxidant (2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox equivalent antioxidant capacity (TEAC), and ferric reducing antioxidant power (FRAP)), antimicrobial, anticancer activities, and the individual carotenoids and anthocyanins content of methanol extracts of the Cyclamen genotypes: Persian cyclamen accessions (Cyclamen persicum Mill.), sowbread (C. mirabile Hildebr.), and ivy-leaved cyclamen (C. hederifolium Mill.) aerial parts. The HPLC-PDA analysis revealed the presence of five individual carotenoids (i.e., neoxanthin, violaxanthin, lutein, β-carotene, and cis-β-carotene) as the main compounds in Cyclamen leaves, and the presence of seven individual anthocycanins (i.e., cyanidin 3,5-di-O-glucoside, peonidin-rutinoside, peonidin 3,5-di-O-glucoside, peonidin 3-O-glucoside, malvidin 3-O-glucoside, malvidin 3,5-di-O-glucoside, and malvidin-rutinoside) in Cyclamen flowers reported, hereby, for the first time. The highest phenolic content was found in the leaves of LC6, C. mirabile (46.32 ± 0.14 mg/g gallic acid equivalents [GAE]), and in the flowers of C. persicum Merengue Magenta (FC15) (58.63 ± 0.17 mg/g GAE), whereas the highest flavonoid content was reported in C. persicum Halios Falbala leaves, namely LC9 (54.90 ± 0.27 mg/g quercetin equivalents [QE]) and in flowers of C. persicum Victora (FC2) (77.87 ± 0.25 mg/g QE). The highest antioxidant activity in DPPH and FRAP assays was reported in C. persicum Dark Violet (LC1) and Victoria (LC2), whereas C. mirabile (LC6) had the highest activity in the TEAC assay. In flowers, high antioxidant activities in DPPH and TEAC were noticed in C. persicum Superserie Red (FC7) and Dark Violet (FC1), respectively, and Halios Falbala (FC9) exhibited the highest activity in the TEAC assay. Additionally, FC9 exhibited the highest antibacterial activity in almost all tested bacteria compared with the leaves extracts. Furthermore, the highest in vitro citotoxicity in MDA-MB-231 cells was noticed in C. hederifolium LC18 (56.71–69.35%) and FC18 (40.07–41.43%), with a lower effect against BJ cells demonstrating selective toxicity. The above findings, highlight the potential use of the Cyclamen flower and leaf extracts as significant anticancer agents along with their antioxidant and antimicrobial properties.

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“…In a study conducted with methanol, ethanol, acetone and petroleum ether extracts of tuber and leaves of C. graecum, the highest antioxidant activity was found in the ethanol extract of the leaf part with 97.3 ± 0.55 % [26]. In the study conducted by Turan and Mammadov [27] with Cyclamen alpinum Dammann ex Spreng, ethanol, methanol and acetone extracts of tuber and leaf parts at 1 mg/mL concentration were used and found the best antioxidant activity in acetone extract of the tuber part with 86.73 ± 0.16 %. In a study conducted with methanol extract of tuber, root, flower and leaves of C. cilicium species collected from Konya province in Turkey, the highest antioxidant activity was found in the root part with 94.28 ± 1.15 mg TE/g value [28].…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and anti-proliferative activities of different parts of Cyclamen cilicium

TURAN

Seçme

Mammadov

2022

Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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This study was designed for investigate of antioxidant, and anti-proliferative activities of acetone, methanol and water extracts of different parts (fresh and underground parts) of Cyclamen cilicium extracts. The antioxidant activities were determined using DPPH, ABTS free radical scavenging activities. Anti-proliferative activities of extracts in Caco-2 colon cancer cells were detected by XTT assay. The determination of contents was evaluated using total phenolics, flavonoid and tannin contents and found maximum values 3.69 ± 0.13 (mg GAE/g-extract), 18.48 ± 0.12 (mg QE/g-extract), 41.17 ± 0.44 (mg CE/g-extract) respectively. C. cilicium extracts exhibited anti-proliferative activity in Caco-2 colon cancer cells. IC50 values of methanol and water extracts of fresh part and water extract of underground part were determined as 35.3276 μg/mL, 42.1831 μg/mL and 64.6144 μg/mL at 24h, respectively. The results showed that C. cilicium is the source of natural antioxidants and may have potential use in pharmaceutical, health, biotechnology, agricultural industry.

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Antioxidant, Antimicrobial, Cytotoxic, Larvicidal and Anthelmintic Activities and Phenolic Contents of <i>Cyclamen alpinum</i>

Cited by 23 publications

References 25 publications

Chemical and pharmacological characterization of aqueous and ethanolic extracts of Cyclamen hederifolium Ait. (Primulaceae) tuber

Chemical and pharmacological characterization of aqueous and ethanolic extracts of Cyclamen hederifolium Ait. (Primulaceae) tuber

Anthocyanins and Carotenoids Characterization in Flowers and Leaves of Cyclamen Genotypes Linked with Bioactivities Using Multivariate Analysis Techniques

Antioxidant and anti-proliferative activities of different parts of Cyclamen cilicium

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