Antioxidant, antiproliferative and antimicrobial activity of freeze-dried raspberry

Abstract

The main chemical composition, i.e. the total content of bioactive compounds (phenolics 2209.86 ? 70.32 mg GAE/100g FDR, flavonoids 831.87 ? 12.61 mg R/100g FDR and anthocyanins 144.55 ? 0.39 mg CGE/100g FDR), in freeze-dried raspberry (FDR) was evaluated spectrophotometrically. Vitamin C content was determined by HPLC analysis (88.81 ? 4.38 mg vit C/100g FDR). Antioxidant activities of FDR extract were evaluated spectrophotometrically on stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and by electron spin resonance spectroscopy (ESR) method on hydroxyl radicals (?OH). EC50 values were evaluated. EC50 DPPH? was 0.127 ? 0.013 mg/ml, while EC50 ?OH was 1.366 ? 0.026 mg/ml. Antiproliferative activity of the FDR extract was evaluated in vitro in three human cell lines by colorimetric sulphorhodamine B (SRB) assay. The most pronounced effects were obtained in the breast adenocarcinoma cell line (MCF7). EC50 value was 395.07 ? 96.38 ?g/ml. Antimicrobial activity was determined by disk diffusion method. The FDR extract produced a clear inhibition zone (without visible colonies) only toward Staphylococcus aureus. The minimal inhibitory (MIC) and minimal bactericidal (MBC) concentrations of FDR extract were evaluated. The values MIC were in the range of 4.7 - 100 mg/ml, and of MBC in the range of 6.3 - > 100 mg/ml.[ Projekat Ministarstva nauke Republike Srbije, br. TR 31044]

Fruit snacks from raspberries: influence of drying parameters on colour degradation and bioactive potential

“…Berry fruits are a rich source of bioactive compounds such as anthocyanins, flavonols and catechins. Numerous studies demonstrated that these phytochemicals exhibit a wide range of biological effects (Paredes‐Lopez et al ., ; Vulić et al ., ). Raspberry is one of the most popular berries in the world, and its exploitation in diverse areas of food and health products is increasing in the last years (Vesna et al ., ).…”

Spray-dried powders from berries extracts obtained upon several processing steps to improve the bioactive components content

“…Berries are a rich source of bioactive compounds such as anthocyanins, flavonols and polyphenols, which contribute to their antioxidant activity and different biological functions [1][2][3]. Anthocyanins have demonstrated promising health-promoting properties like anti-inflammatory, immunomodulatory, antimicrobial, improvement of vision and neuroprotective effects [4][5][6][7][8].…”

Biowaste as a Potential Source of Bioactive Compounds—A Case Study of Raspberry Fruit Pomace

“…The obtained results are in good agreement with the previously published data from studies that investigated RFP and lyophilized fruit extracts (which ranged from 2.34 to 43.43 mg/g GAE for TPC and 5.19 to 16.35 mg/g QE for TFC). However, the TAC values in the analyzed RFP using both extraction methods showed better results than those of raspberry pomace extracted from lyophilized fresh (29.69–81.13 mg/100 g C3G EQ), as well as fresh-frozen raspberry fruit (144.55 mg/100 g C3G) extracted by maceration with 80% methanol [ 36 , 38 ]. The TPC values determined for RFP extracts are similar to those reported by Brodowska [ 19 ] (591.69 mg/100 g fresh pomace) and are in good agreement with blackberry ( Rubus fruticosus L.) pomace TPC results [ 37 , 39 ].…”