Antioxidant Assay Guided Separation of the Methanol Stem Fraction of Binahong (Anredera Cordifolia) using Cyclic Voltammetry Method

Abstract

The aim of this study was to identify and characterize groups of antioxidant active naturally from methanol extract of the stem of Anredera cordifolia through antioxidant assay-guided separation. The methanol extract (ME) displayed the highest antioxidant activity, compared to ethyl acetate extract (EE) and hexane extract (HE) using cyclic voltammetry method. Ascorbic acid was used as a positive control in this research. The repeated fractionation of active ME by MPLC and HPLC analyses yielded a constituent with strong antioxidant property. The active constituent (RNV-1) was predicted as a flavonoid type based on the UV spectrum data. The antioxidant activity results exhibited that RNV-1 had a coefficient of antioxidant activity (K) sligtly higher than K of ascorbic acid with the values of 0.055 and 0.052 respectively. It was found that RNV-1 demonstrated an excellent antioxidant activity in comparison with the standard antioxidant activity through antioxidant assays-guided separation. The results obtained suggested that the stem of A. cordifolia can be used potentially as a source of natural antioxidants by contributing beneficial health effects.