Antioxidant constituents from Lawsonia inermis leaves: Isolation, structure elucidation and antioxidative capacity

Hsouna, Anis Ben; Trigui, Mohamed; Culioli, Gérald; Blache, Yves; Jaoua, Samir

doi:10.1016/j.foodchem.2010.08.060

Cited by 97 publications

(57 citation statements)

References 25 publications

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“…The AHE contained about 580 mg GAE/g of extract, which was higher than that reported by Hsouna et al [26] (444 mg GAE/g of extract). In addition, a total 48 volatile components (aliphatic compounds, terpenoids, aromatics, sesquiterpenes, aldehydes, carboxylic acids, monoterpenes, etc.)…”

Section: Analysis Of Volatile Compoundscontrasting

confidence: 65%

“…Therefore, it could be concluded that free radical species scavenging is an important mechanism, by which antioxidant compounds improve cell viability against oxidation-induced cell death. In addition, as previously demonstrated by Hsouna et al [26], some of the constituents of henna could act as free radical scavengers, preventing phospholipid membrane from peroxidation and protecting immuno-compromised cells from free radical damage.…”

Section: Tissue Antioxidant Enzyme Activities and Mda Contentsupporting

confidence: 52%

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Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract

Jridi

Sellimi

Lassoued

et al. 2017

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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The in vivo wound-healing potential of gels and films based on cuttlefish skin gelatin (CSG) incorporated with aqueous henna extract (AHE) (at 50 and 500 µg/ml) was studied.Since wound healing is related to reactive oxygen species (ROS), this study was first conducted to evaluate the antioxidant activity of AHE by DPPH radical-scavenging, β-carotene-linoleate bleaching, and reducing power assays. The major volatile compounds were lawsone, dimethyl sulfoxide, dimethyl sulfide, eugenol and maltol. The finding of the study showed that the incorporation of AHE into gelatin gels and films enhanced the antioxidant activity in a dose-dependent manner. In the wound-healing study, topical application of gelatin hydrogels or films enriched by AHE, on the wound site in a rat model, enhanced significantly wound healing activity and helped to prevent from inflammation damage, when compared to the control and CICAFLORA®-treated groups. Further, all preparations were found to improve the antioxidant status of treated rats as was evidenced by increased antioxidant enzymatic activities and reduced MDA content. The increase in the activities of catalase (CAT), superoxide dismutase (SOD) and gluthatione peroxydase (GPx) in wound tissues revealed that bioactive compounds in AHE, film and gel are able to enhance the healing of wounds by reducing the damage caused by ROS. Wound-healing activity was confirmed by histopathology studies. The obtained results showed that the addition of AHE to the gelatin matrix could improve the wound healing activity.

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Section: Analysis Of Volatile Compoundscontrasting

confidence: 65%

Section: Tissue Antioxidant Enzyme Activities and Mda Contentsupporting

confidence: 52%

Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract

Jridi

Sellimi

Lassoued

et al. 2017

Colloids and Surfaces A: Physicochemical and Engineering Aspect

View full text Add to dashboard Cite

show abstract

“…The herb has been reported to exhibit anti-oxidant, anti-microbial, antiviral, anti-cancer, anti-coagulant, wound-healing, abortifacient, analgesic and anti-inflammatory activities. There have been research evidences to ascertain the antioxidant effects of Lawsonia inermis due to the presence of various phytonutrients including flavonoids, tannins, terpenoids and coumarins [8]. On top of its antioxidants potential, Lawsonia inermis has been reported to exhibit anti-inflammatory, antibacterial, antifertility, wound healing and anticancer activities [7,9].…”

Section: Introductionmentioning

confidence: 99%

In-vitro Assays to Show the Antioxidant Potential of ?-sitosterol from Lawsonia inermis Leaves

2016

IJSR

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Apprehensions about the therapeutic efficacy and safety of synthetic drugs fuel the research to discover holistic remedies. The antioxidant effect of methanolic extract of Lawsonia inermis leaves (MELIL) -sitosterol have been investigated against the in-vitro oxidant systems by using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical, superoxide anion, FRAP (ferric reducing antioxidant power) and nitric oxide assays. The results obtained clearly shows that -sitosterol exerts significant antioxidant effect across all the studied oxidant systems.

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“…phase I and II enzymes (Dasgupta et al 2003). Extracts as well as various pure constituents from this plant have been reported with antioxidant (Hsouna et al 2011;Guha et al 2011;Kumar et al 2014), antimutagenic, antigenotoxic, anticlastogenic (Raja et al 2008;Basirian et al 2013;Kumar et al 2014) and anitumor activity (Dasgupta et al 2003;Priya et al 2011). From the last 25 years, our laboratory have been studying medicinal plants for their bioactive potential.…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative and Apoptosis Inducing Effects of Non-Polar Fractions from Lawsonia inermis L. in Cervical (HeLa) Cancer Cells

Kumar

Kaur

Kumar

et al. 2015

Physiol Mol Biol Plants

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Two non-polar fractions viz. hexane (Hex-LI) and chloroform fraction (CHCl 3 -LI) of Lawsonia inermis were studied for their antiproliferative potential in various cancer cell lines viz. HeLa, MCF-7, A549 and C6 glioma cells. Both the fractions showed more than 60 % of growth inhibition in all the tested cell lines at highest tested concentration. In clonogenic assay, different concentrations of Hex-LI and CHCl 3 -LI decreased the number and size of colonies as compared to control in HeLa cells. The apoptotic effects as nuclear condensation, fragmentation were visualized with Hoechst-33342 staining of HeLa cells using confocal microscope. Both fractions induced apoptotic cell death in human cervical carcinoma (HeLa) cells as evident from flow cytometric analysis carried out using Annexin V-FITC and propidium iodide dyes. CHCl 3 -LI treated cells significantly induced apoptosis (25.43 %) in comparison to control. Results from Neutral Comet assay demonstrated that both fractions induced double stranded breaks (DSB's) in HeLa cells. Our data indicated that Hex-LI and CHCl 3 -LI treated cells showed significant increase of 32.2 and 18.56 % reactive oxygen species (ROS) levels in DCFH-DA assay respectively. Further, experimental studies to decipher exact pathway via which these fractions induce cell death are in progress.

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Antioxidant constituents from Lawsonia inermis leaves: Isolation, structure elucidation and antioxidative capacity

Cited by 97 publications

References 25 publications

Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract

Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract

In-vitro Assays to Show the Antioxidant Potential of ?-sitosterol from Lawsonia inermis Leaves

Antiproliferative and Apoptosis Inducing Effects of Non-Polar Fractions from Lawsonia inermis L. in Cervical (HeLa) Cancer Cells

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