Antioxidant Constituents of Cotoneaster melanocarpus Lodd.

Abstract

The aim of this study was the evaluation of the antioxidant capacity of Cotoneaster melanocarpus Lodd. and the identification of antioxidant active constituents of this plant. C. melanocarpus Lodd. is a shrub indigenous to Mongolia and used in Traditional Mongolian Medicine as a styptic. Before extraction, the plant material was separated into three parts: young sterile shoots, older stems and leaves. All these parts were extracted with water, methanol, ethyl acetate, dichloromethane and hexane, successively. The methanolic extract of the sterile shoots showed the highest antioxidant activity in the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay (IC50 30.91 ± 2.97 µg/mL). This active extract was further analyzed with chromatographic methods. TLC fingerprinting and HPLC indicated the presence of the flavonol glycosides quercetin-3-O-rutinoside (rutin), quercetin-3-O-galactoside (hyperoside) and quercetin-3-O-glucoside (isoquercetin), ursolic acid as well as chlorogenic acid, neochlorogenic acid and cryptochlorogenic acid. The findings were substantiated with LC-MS. All identified compounds have antioxidant properties and therefore contribute to the radical scavenging activity of the whole plant.

Contribution of Individual Polyphenols to Antioxidant Activity of Cotoneaster bullatus and Cotoneaster zabelii Leaves—Structural Relationships, Synergy Effects and Application for Quality Control

“…The genus Cotoneaster Medikus (Rosaceae) comprises about 500 species native to central and southern China and naturalized in Europe, where they are extensively cultivated as ornamental plants. Various Cotoneaster representatives are used in traditional Asian medicine for their cardiotonic, hypotensive, diuretic, antispasmodic, expectorant, astringent and antiviral properties, and are indicated, e.g., for cardiac complaints, diabetes mellitus, nosebleeds, hematemesis and excessive menstruation [1][2][3]. Based on the previous studies, phenolics from the group of mono-and oligomeric flavan-3-ols, quinic acid pseudodepsides and flavonol glycosides may be responsible for these activities [2,[4][5][6][7].…”

Hyperin protects against cisplatin-induced liver injury in mice

“…Hyperin, a flavonoid compound occurring in natural plants, has been demonstrated to exert a variety of biological activities, including antioxidant 11,12 , anti-inflammatory 13 , anticancer 14 , and cardiovascular protective effects 15 . Besides, hyperin is reported to have hepato-protective activity 16,17 .…”

Phytochemicals in leaves of Cotoneaster mongolica, their antioxidative, and acetylcholinesterase inhibitory activity

“…varied between EC 50 , 18.5-34.5 μg/mL [13]. The leaf methanol extract of C.melanocarpus showed activity with IC 50 , 106.41 μg/mL [4]. The twig ethanol extract of C.horizontalis possessed high scavenging activity of IC 50 ,19.3 μg/mL [14], compared to the same crude drug methanol extracts of C.nummularia (IC 50 , 104.0 mg/mL) [15] and C.integerrimus (IC 50 , 1.06 mg/mL) [16].…”

“…Thus, the biological activity of extracts and fractions depends on the complex nature of phytochemicals and their synergistic and antagonistic effects [15]. In this study, 12 compounds have been isolated from the n-butanol fraction, including quercetin (1) [18,19] [13,4]. In addition, quercetin (1) was identified in the twig of C.integerrimus [16], and ursolic acid (5), corosolic acid (6), euscaphic acid (7) and arbutin (12), respectively were identified in the leaves of C.simonsii by HPLC analysis [31].…”

“…Sepra TM C18-E (50 μm, 65Å) were used for column chromatography (CC). NMR spectra were recorded on Bruker AM-300 Spectrometer in MeOH-d 4 1 H (300 MHz) and 13 C (75 MHz). UV spectroscopic analysis was carried on a spectrophotometer UV-160 (Shimadzu, Japan).…”