Antioxidant, Cytotoxic, and Antiproliferative Activities and Total Polyphenol Contents of the Extracts of Geissospermum reticulatum Bark

Abstract

Geissospermum species are medically important plants due to their health-promoting effects. The objective of this study was to determine the antioxidant ability and antiproliferative and cytotoxic effects of infusions, tinctures, and ethanolic extracts of Geissospermum reticulatum barks in relation to the contents of total phenolics and flavonoids. Seven samples of barks were collected in various regions of Peruvian Amazonia. We found that the amount of total phenolics in the studied products varied from 212.40 ± 0.69 to 1253.92 ± 11.20 mg GAE/kg. In our study there is a correlation (R 2 = 0.7947) between the results of antioxidants assays: FRAP and ORAC for tinctures, infusions, and ethanolic extracts of G. reticulatum barks. We have also observed antiproliferative activities of the ethanolic extracts on normal T-cells. These extracts have caused death on malignant cell lines (THP-1 and HL-60) and this data correlates well with their antioxidant capacity measured by ORAC method. Interestingly, the highest concentration of the ethanolic extract was not toxic in the zebrafish embryo developmental assay. Our results indicate that G. reticulatum is rich in antioxidants and have cytotoxic and antiproliferative properties. The data suggests potential immunosuppressive role of the extracts. This is the first study presenting the results of chemical and biological analysis of multiple preparations from G. reticulatum.

Role of Plant Derived Alkaloids and Their Mechanism in Neurodegenerative Disorders

“…Aqueous infusions of its bark are frequently used by native societies for countless medicinal determinations. It is medically significant plant and exerts a round figure of medicinal potentials as anti-oxidant 109 as well as antibacterial and antimalarial 110 , 111 .…”

Ethnopharmacological and botanical evaluation of medicinal plants used by Brazilian Amazon Indian community

“…Thus, Vatairea macrocarpa has been shown to have compounds capable of suppressing the synthesis of pro-inflammatory mediators (ALENCAR et al, 2006). Studies on the phytochemical and pharmacological properties of Geissospermum reticulatum have shown that extracts of this plant are provided with anti-tumor and antioxidant activity (SAJKOWSKA-KOZIELEWICZ et al, 2016). Regarding the genus Ampelozizyphus, it has been shown that the aqueous extract of A. amazonicus is immunomodulatory and anti-inflammatory (PEÇANCHA et al, 2013).…”

Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark

“…Geissospermum species (Apocynaceae) grow in the Amazonian rainforest [ 1 , 2 ]. For many years they have proven popular as therapeutic plants used in traditional medicines as remedies for malarial, tumors, bacterial infections, and pain relief, and as anti-inflammatory agents [ 1 , 3 , 4 ]. First investigations of the chemical composition of these trees date back to the end of the 19th century [ 1 ].…”