Antioxidant Defenses and DNA Damage Induced by UV‐A and UV‐B Radiation in the Crab <i>Chasmagnathus granulata</i> (Decapoda, Brachyura)<sup>¶</sup>

Gouveia, Glauce Ribeiro; Marques, Daiane S.; Cruz, Bruno Pinto; Geracitano, Laura A.; Nery, Luiz Eduardo Maia; Trindade, Gilma Santos

doi:10.1111/j.1751-1097.2005.tb00200.x

Cited by 8 publications

(8 citation statements)

References 31 publications

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“…Marine arthropods (i.e., crabs, lobsters, and shrimp) vary in their antioxidant defenses according to their level of aerobic metabolism, exposure to chronically cold environments, or exposure to UVR (111)(112)(113). Surprisingly, marine arthropods lack Cu/Zn SOD (114,115).…”

Section: Oxidative Stress In Marine Organismsmentioning

confidence: 99%

OXIDATIVE STRESS IN MARINE ENVIRONMENTS: Biochemistry and Physiological Ecology

Lesser

2006

Annu. Rev. Physiol.

1,501

1,061

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Oxidative stress-the production and accumulation of reduced oxygen intermediates such as superoxide radicals, singlet oxygen, hydrogen peroxide, and hydroxyl radicals-can damage lipids, proteins, and DNA. Many disease processes of clinical interest and the aging process involve oxidative stress in their underlying etiology. The production of reactive oxygen species is also prevalent in the world's oceans, and oxidative stress is an important component of the stress response in marine organisms exposed to a variety of insults as a result of changes in environmental conditions such as thermal stress, exposure to ultraviolet radiation, or exposure to pollution. As in the clinical setting, reactive oxygen species are also important signal transduction molecules and mediators of damage in cellular processes, such as apoptosis and cell necrosis, for marine organisms. This review brings together the voluminous literature on the biochemistry and physiology of oxidative stress from the clinical and plant physiology disciplines with the fast-increasing interest in oxidative stress in marine environments.

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Section: Oxidative Stress In Marine Organismsmentioning

confidence: 99%

OXIDATIVE STRESS IN MARINE ENVIRONMENTS: Biochemistry and Physiological Ecology

Lesser

2006

Annu. Rev. Physiol.

1,501

1,061

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“…Therefore, the purpose of this study has been, first, to confirm apoptosis under the same conditions used previously with the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) technique, and then to test, in the visual system of this same species, whether catalase, Bax, and p53 expression (by using immunohistochemistry) and levels (through immunoblotting) are affected by the same high dose of UVB and UVC radiation used previously. A remark should be added here regarding the focus of this study: even though the effects caused by UVA and UVB have received more attention than those produced by UVC (Sanford et al 1996;Duncan et al 1997;Gouveia et al 2005), the latter should not be neglected, because, among other reasons, the study of UVC radiation may help us to understand some of the underlying mechanisms of UV-induced cell damage.…”

Section: Introductionmentioning

confidence: 96%

Catalase, Bax and p53 expression in the visual system of the crab Ucides cordatus following exposure to ultraviolet radiation

et al. 2007

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In invertebrates, a few studies have suggested apoptosis as the mechanism of choice to protect the retina after exposure to ultraviolet (UV) radiation. We demonstrated previously, by electron microscopy, that the retina and lamina ganglionaris (or lamina) cells of the crab Ucides cordatus displayed subcellular signs of apoptosis after exposure to UVB and UVC. Here, we first ascertained, by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) technique, that UV irradiation indeed produced the previously reported results. We next tested, in the visual system of U. cordatus, whether the expression (as analyzed by immunohistochemistry and observed with laser scanning microscopy) and levels (as examined by Western blotting) of catalase, Bax, and p53 were affected by the same dose of UV radiation as that used previously. Our data revealed that the intensity of catalase, Bax, and p53 labeling was stronger in irradiated retina and lamina cells than in non-irradiated retina and lamina. However, no significant difference was observed in the concentrations of these proteins isolated from the whole optic lobe. The results thus suggest that UVB and UVC induce apoptosis in the crustacean retina and lamina by increasing catalase expression and activating the Bax- and p53-mediated apoptosis pathways.

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“…Patton et al (1999) also showed higher DNA damage in bovine retinal pigment epithelium cells (RPE) irradiated with UVB (0.012 J cm −2 ). At the highest dose of UVB (0.075 J cm −2 ), nuclei in the slides of the comet assay were not visible, suggesting that there may be a somatic effect of UVB radiation and the comet assay protocol, which may lead to cell destruction (Gouveia et al 2005). However, Patton et al (1999), utilizing higher doses of UVB (0.045 and 0.09 J cm −2 ), achieved to visualize nuclei and DNA damage in RPE cells.…”

Section: Highmentioning

confidence: 89%

“…While UVC is essentially completely absorbed by oxygen and is thus ecologically irrelevant, the longer UVB and UVA wave lengths ate relevant and can induce a variety of effects on many organisms. The deleterious effects of UVR range from molecular and tissue damage to population level Communicated by Wayne S. Gardner * José Henrique Muelbert docjhm@furg.br effects (Wake 1991;Kerr and McElroy 1993;Blaustein et al 1994;Bothwell et al 1994;Hofer and Mokri 2000;Ravanat et al 2001;Armstrong et al 2002) including damage to relevant biological molecules such as proteins, lipids, and DNA (Sinha and Hader 2002;Gouveia et al 2005).…”

Section: Introductionmentioning

confidence: 99%

UVA and UVB Penetration in the Water Column of a South West Atlantic Warm Temperate Estuary and its Effects on Cells and Fish Larvae

Gouveia

Trindade

Nery

et al. 2015

Estuaries and Coasts

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The objective of this study was to determine the seasonal incidence of ultraviolet A (UVA) and ultraviolet B (UVB) in the water column of the Patos Lagoon estuary and the effects of UV radiation (UVR) at different organizational levels. Field work was conducted to collect UVA and UVB radiance, transparency, salinity, seston and chlorophyll a (Chl a) concentration, and fish larvae at an inner and at an outer sampling site in the Patos Lagoon estuary (32°S). Cell culture techniques combined with tools for fluorimetric detection were used to assess possible damage caused by UVR. GEM-81 erythrophoroma cells and larvae of silverside Odontesthes argentinensis were used as biological indicators. Field results revealed that at the inner station, UVA penetration was deeper in autumn and shallower in spring when water-column was less transparent, fresher, and had the highest concentration of seston and Chl a. At the outer station, UVA penetration in the water-column in spring was significantly different than in summer, when transparency and water salinity were high and Chl a was low. UVB did not present significant variation between seasons at both sampling stations. Experiments with GEM-81 cell lineage revealed that UVA doses similar to the environment (1.35 J cm −2 ) inhibited cell proliferation, increased the concentration of reactive oxygen species (ROS) and the lipid peroxide content (LPO), and lowered the activity of the antioxidant defense system (total antioxidant capacity against peroxyl radicals (TOSC)). For UVB, 0.075 J cm −2 caused cytotoxicity and 0.015 J cm −2 induced DNA damage in cells. Silverside larvae showed low growth rate when exposed to UVA and low antioxidant capacity when exposed to UVB. These results suggest that planktonic organisms that live close to surface water column in the Patos Lagoon estuary are subject to the effects of continued exposure to UVR. Knowledge of this impact is relevant to understand future changes in the populations dynamics of planktonic organisms and its ecological and economic implications to southern Brazil.

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Antioxidant Defenses and DNA Damage Induced by UV‐A and UV‐B Radiation in the Crab Chasmagnathus granulata (Decapoda, Brachyura)^¶

Cited by 8 publications

References 31 publications

OXIDATIVE STRESS IN MARINE ENVIRONMENTS: Biochemistry and Physiological Ecology

OXIDATIVE STRESS IN MARINE ENVIRONMENTS: Biochemistry and Physiological Ecology

Catalase, Bax and p53 expression in the visual system of the crab Ucides cordatus following exposure to ultraviolet radiation

UVA and UVB Penetration in the Water Column of a South West Atlantic Warm Temperate Estuary and its Effects on Cells and Fish Larvae

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Antioxidant Defenses and DNA Damage Induced by UV‐A and UV‐B Radiation in the Crab Chasmagnathus granulata (Decapoda, Brachyura)¶

Cited by 8 publications

References 31 publications

OXIDATIVE STRESS IN MARINE ENVIRONMENTS: Biochemistry and Physiological Ecology

OXIDATIVE STRESS IN MARINE ENVIRONMENTS: Biochemistry and Physiological Ecology

Catalase, Bax and p53 expression in the visual system of the crab Ucides cordatus following exposure to ultraviolet radiation

UVA and UVB Penetration in the Water Column of a South West Atlantic Warm Temperate Estuary and its Effects on Cells and Fish Larvae

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Antioxidant Defenses and DNA Damage Induced by UV‐A and UV‐B Radiation in the Crab Chasmagnathus granulata (Decapoda, Brachyura)^¶