Antioxidant effect of a novel class of telluroacetilene compounds: Studies in vitro and in vivo

Souza, Ana Cristina Guerra; Luchese, Cristiane; Neto, José S. S.; Nogueira, Cristina W.

doi:10.1016/j.lfs.2008.12.021

Cited by 30 publications

(32 citation statements)

References 54 publications

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“…As such, the crucial role of this enzyme in neural transmission makes it a primary target for a large number of cholinesterase-inhibiting drugs and toxins (Pohanka, 2011), and a valuable diagnostic tool for verifying exposure to chemical agents (Shenouda, Green, Sultatos, 2009). The heme pathway enzyme deltaaminolevulinate dehydratase (δ-ALA-D, EC 4.2.1.24) presents high sensitivity to pro-oxidant situations, and to impairment of metabolic processes, thus being used to evaluate toxicity as well (Nogueira et al, 2003;Souza et al, 2009). Previous studies by our group have already demonstrated the inhibitory in vitro effects of S. guaraniticum extract on erythrocyte and brain δ-ALA-D, suggesting possible extract caused hematological and central nervous systems impairments (Bonfanti et al, 2013(Bonfanti et al, , 2014.…”

Section: Introductionmentioning

confidence: 99%

Safety assessment and behavioral effects of Solanum guaraniticum leaf extract in rats

Bonfanti

Bitencourt

Bona

et al. 2016

Braz. J. Pharm. Sci.

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Solanum guaraniticum is a medicinal plant traditionally used to treat gastric and liver diseases. However, there is no documented evidence corroborating its safety. The present study evaluated the potential toxicity of S. guaraniticum leaf extract after acute administration in rats. Single doses of the extract (1.250, 2.500, and 5.000 mg/kg) were administered by gavage, and the rats were then monitored for 48 h and/or 14 days. Mortality, acute signs of toxicity, and general activity in the open field test were assessed as well as hematological and biochemical parameters, enzymatic activity (δ-aminolevulinate dehydratase and acetylcholinesterase), and oxidative stress parameters (lipid peroxidation level, nonprotein thiol content, tissue catalase activity, and serum ferrous reducing power). Phytochemical analysis was also performed by HPLC. The results showed that extract administration produced no deaths (LD 50 > 5,000 mg/kg), and no significant adverse effects regarding food consumption, body weight gain, gross pathology, or other parameters. However, the open field tests showed a decrease in spontaneous activity (crossing and rearing) mainly at 48 h after treatment. The results suggest that S. guaraniticum extract is not acutely toxic, but causes alterations in central nervous system activity.Uniterms: Solanum guaraniticum/leaf extract/toxicity. Solanum guaraniticum/phytochemistry. Medicinal plants. Solanaceae.Solanum guaraniticum é uma planta medicinal tradicionalmente usada para tratar doenças gástricas e hepáticas. Porém, não há evidências documentadas sobre sua segurança. O presente estudo avaliou a toxicidade do extrato das folhas de S. guaraniticum após administração aguda em ratos. Doses únicas do extrato (1.250, 2.500 and 5.000 mg/kg) foram administradas por gavagem e os animais foram monitorados por 48 h ou 14 dias. Mortalidade, sinais de toxicidade aguda e atividade geral, através do teste de campo aberto, foram analisados, assim como parâmetros hematológicos e bioquímicos, atividades enzimáticas (δ-aminolevulinato desidratase e acetilcolinesterase) e parâmetros de estresse oxidativo (nível de peroxidação lipídica, conteúdo de tióis não protéicos, atividade da catalase em tecidos e poder redutor em soro). A análise fitoquímica também foi realizada por HPLC. Os resultados mostraram que a administração do extrato não provoca mortes (LD 50 >5.000 mg/kg) ou efeitos adversos significativos com relação ao consumo de comida, ganho de peso corporal, análise patológica, entre outros. Entretanto, o teste de campo aberto mostrou uma diminuição na atividade espontânea geral (cruzamentos e levantadas), principalmente em 48 h após o tratamento. Portanto, nossos resultados sugerem que o extrato de S. guaraniticum não é agudamente tóxico, mas causa alterações na atividade do sistema nervoso central. Unitermos:Solanum guaraniticum/extrato de folhas/toxicidade. Solanum guaraniticum/fitoquímica. Plantas medicinais. Solanaceae.

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Section: Introductionmentioning

confidence: 99%

Safety assessment and behavioral effects of Solanum guaraniticum leaf extract in rats

Bonfanti

Bitencourt

Bona

et al. 2016

Braz. J. Pharm. Sci.

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“…Moreover, it is difficult to clearly explain why these compounds did not show enzyme-like activity. Accordingly, other studies have revealed that some organochalcogen compounds with antioxidant activity did not exhibit thiol peroxidase-like activity (Souza et al 2009, Gay et al 2010.…”

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confidence: 97%

“…WILHELM et al cancer, atherosclerosis, rheumatoid arthritis, hypertension, ischemia and diabetes mellitus (Souza et al 2009, Rajendran et al 2014. In this sense it is important to use antioxidant molecules that decrease oxidative stress, reducing the incidence of various diseases (Gay et al 2010, Maes et al 2011, Gupta et al 2014.…”

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confidence: 99%

Antioxidant effect of quinoline derivatives containing or not selenium: Relationship with antinociceptive action quinolines are antioxidant and antinociceptive

Wilhelm

FERREIRA

Pinz

et al. 2017

An. Acad. Bras. Ciênc.

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The present study investigated the antioxidant effect of a new class of quinoline derivatives (a-d) on assays in vitro. Lipid peroxidation, thiol peroxidase-like and free radical scavenging activities were determined to evaluate antioxidant activity of compounds. Thiol oxidase-like and δ-aminolevulinate dehydratase activities were performed as a toxicological parameter. A second objective of this study was to evaluate the in vivo antinociceptive effect of the compound with better antioxidant effect and without toxic effects in a model of nociception induced by formalin in mice. In liver, at 100 µM, compound a reduced the lipid peroxidation to the control levels, while compounds c and d partially reduced it. In brain, only compound d partially reduced the lipid peroxidation at 50 and 100 µM. Compound b did not have an effect on the lipid peroxidation. Thiol peroxidase-like and free radical scavenging activities are not involved in the antioxidant mechanisms of these compounds. Compounds did not present thiol oxidase-like activity and effect on the δ-aminolevulinate dehydratase. In vivo experiments showed that compound a caused an inhibition of licking time in the first and second phases, and edema formation induced by formalin. In conclusion, quinoline derivative without selenium presented better in vitro antioxidant effect and in vivo antinociceptive activity.

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“…In fact, -ALA-D is a group of sulfhydrylcontaining enzymes 50 , being extremely sensitive to the presence of pro-oxidant elements, and numerous compounds that oxidize sulfhydryl groups, such as selenium and tellurium compounds 39,51,52 , modify its activity. Particularly, organotellurium compounds may interact with thiol groups of this enzyme, oxidizing sulfhydryl groups of the active site, changing the configuration of the enzyme and impairing its activity.…”

Section: Discussionmentioning

confidence: 99%

“…Organotellurides A-D showed thiol oxidase activity only at the concentration of 100 μM, indicating an advantage regarding the possible toxicological properties of these compounds. Recently, our research group reported that the mechanism involved in -ALA-D inhibition caused by telluroacetylenes in vitro is related to their ability to oxidize sulfhydryl groups from the enzyme structure 52 . In conformity, our results demonstrated an inverse correlation between thiol oxidase and -ALA-D activities, meaning that -ALA-D activity decreases as the compound's ability to oxidize sulfhydryl groups increases.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant effect of functionalized alkyl-organotellurides: a studyin vitro

Luchese

Nogueira

Wendler

et al. 2010

Journal of Enzyme Inhibition and Medicinal Chemistry

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Antioxidant effect of a novel class of telluroacetilene compounds: Studies in vitro and in vivo

Cited by 30 publications

References 54 publications

Safety assessment and behavioral effects of Solanum guaraniticum leaf extract in rats

Safety assessment and behavioral effects of Solanum guaraniticum leaf extract in rats

Antioxidant effect of quinoline derivatives containing or not selenium: Relationship with antinociceptive action quinolines are antioxidant and antinociceptive

Antioxidant effect of functionalized alkyl-organotellurides: a studyin vitro

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